Sample enrichment by using monolithic precolumns in microcolumn liquid chromatography

Lim, Lee Wah; Hirose, Katsuaki; Tatsumi, Shinichi; Uzu, Hideyuki; Mizukami, Megumi; Takeuchi, Toyohide

doi:10.1016/j.chroma.2004.01.045

Cited by 30 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They clearly indicate that the monolith did not loose its sorption capacity even at very high flow rate of 50 L/min, which enabled very fast runs. A significant 50-fold increase in sensitivity of the in-tube [71]. They observed a significant increase in the UV signal intensity in HPLC separation of phthalates after their enrichment from 20 ng/mL solution.…”

Section: Solid-phase Extraction Coupled To Hplcmentioning

confidence: 87%

Less common applications of monoliths: Preconcentration and solid-phase extraction

Švec

2006

Journal of Chromatography B

196

120

View full text Add to dashboard Cite

Section: Solid-phase Extraction Coupled To Hplcmentioning

confidence: 87%

Less common applications of monoliths: Preconcentration and solid-phase extraction

Švec

2006

Journal of Chromatography B

196

120

View full text Add to dashboard Cite

“…Special grade benzene was purchased from Wako Pure Chemical Industry (Osaka, Japan), while laboratory use benzene-1,3,5-d 3 and benzene-d 6 were obtained from Aldrich Chemical Co. (Milwaukee, WI, USA). Tetramethoxysilane (TMOS) and tetrachlorosilane were purchased from Tokyo Chemical Industry (Tokyo, Japan).…”

Section: Reagentsmentioning

confidence: 99%

Separation of benzene and deuterated benzenes by reversed‐phase and recycle liquid chromatography using monolithic capillary columns

Lim¹,

Uzu²,

Takeuchi³

2004

J of Separation Science

Self Cite

View full text Add to dashboard Cite

An alternate pumping-recycle system utilizing a commercially available low dead-volume switching valve was developed for microcolumn LC. The recycle system had two separation columns, and the dead volume of the recycling lines was kept to a minimum by avoiding passage of the sample through the pump chamber, sample injector, and the normal path length of a conventional UV detector. The drawback of the high total back pressure caused by the second column that is placed after the detector was overcome by on-column detection, and this eliminated the need for a high pressure flow cell. The system was used for the separation of an authentic mixture of benzene, benzene-1,3,5-d3, and benzene-d6. Baseline separation was accomplished after six cycles and the calculated theoretical plate number for benzene was 230,000. It was observed that the theoretical plate number (N) increased linearly with increasing number of cycles, and the N per unit time increased with increasing inlet pressure. The separation conditions were optimized and the separation of benzene and benzene-d6 was accomplished within 75 min at 2.5 MPa inlet pressure.

show abstract

“…The on-column method gave a 50-fold improvement in sample injection while the precolumn allowed injection of up to 5000-fold, which was the highest factor of enrichment that has been reported [23]. Besides C18, C30, IC-Anion-SW particles, monolithic C18 stationary as well as quartz fiber coated with C30 were also used as the precolumn enrichment materials.…”

Section: On-line Sample Enrichment Systemmentioning

confidence: 99%

“…However, decreasing the size of the separation column also decreases its concentration sensitivity due to the limit in the sample injection. In order to overcome this drawback, sample enrichment technique has been proven to be useful and is becoming crucial especially in trace analysis of environmental pollutants [21][22][23][24][25].…”

Section: On-line Sample Enrichment Systemmentioning

confidence: 99%

“…Recently, micro unions and valves with low dead volume are commercially available in the market. By taking the advantages of the capillary system, these accessories can be easily applied into the capillary LC systems without affecting the column separation efficiency, and allowing us to conduct simple stepwise gradient elution [20], precolumn enrichment [21][22][23][24][25] and recycle separation [26], etc., with relatively good efficiency and tolerable band broadening.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of Micro-Flow-Controlled Techniques and Novel Stationary Phases in Capillary Liquid Chromatography

Lim

2015

Chromatography

Self Cite

View full text Add to dashboard Cite

This focusing review summarizes our recent works on the dissemination of capillary liquid chromatography through various approaches, i.e. simple stepwise-gradient elution system, on-line sample enrichment system, alternate-pumping recycle chromatography system, on-line immobilized-enzyme reactor, development of novel stationary phases, etc. Capillary liquid chromatography saves energy and waste, and it possesses several advantages over the conventional liquid chromatography; however, it has not been much popularized since its appearance and most users still prefer to use conventional liquid chromatography if there is a choice between them. Our research focuses on, but not limited to, developing versatile capillary liquid chromatography systems that are not only environmental-friendly but also user-friendly, and novel stationary phases that could be operated under mixed modes conditions by manipulating the eluent, which are applicable to real samples analyses and will therefore eventually switch the users from "conventional" to "capillary".

show abstract

Sample enrichment by using monolithic precolumns in microcolumn liquid chromatography

Cited by 30 publications

References 16 publications

Less common applications of monoliths: Preconcentration and solid-phase extraction

Less common applications of monoliths: Preconcentration and solid-phase extraction

Separation of benzene and deuterated benzenes by reversed‐phase and recycle liquid chromatography using monolithic capillary columns

Development of Micro-Flow-Controlled Techniques and Novel Stationary Phases in Capillary Liquid Chromatography

Contact Info

Product

Resources

About