Sample size and retention values in high-performance liquid chromatography of biological and synthetic polymers

Engelhardt, H.; Czok, M.; Schultz, R.; Schweinheim, E.

doi:10.1016/s0021-9673(00)90555-7

Cited by 18 publications

(3 citation statements)

References 14 publications

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“…Earlier literature often reports on the large effect of sample load on peak shapes and retention times for the gradient elution of macromolecules. ,,, For example, a 250-mm × 4.6-mm column packed with 15-nm pore size C18 silica became overloaded when a 50-μg sample of polystyrene was used, and the overload led to a decrease in the retention . In contrast to this packed column, the molded rod column tolerates much higher sample loads.…”

Section: Resultsmentioning

confidence: 99%

Molded Monolithic Rod of Macroporous Poly(styrene-co-divinylbenzene) as a Separation Medium for HPLC of Synthetic Polymers: “On-Column” Precipitation−Redissolution Chromatography as an Alternative to Size Exclusion Chromatography of Styrene Oligomers and Polymers

et al. 1996

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A process for the separation of styrene oligomers and polymers by size and composition using a novel separation medium has been demonstrated. The process involves precipitation of the macromolecules on the molded macroporous rod columns, followed by progressive elution utilizing a simple gradient of the mobile phase. Molded macroporous rod columns are ideally suited for this technique because convection through the large pores of the rod enhances the mass transport of large analyte molecules and accelerates the separation process. Styrene oligomers and polymers are separated in a 50-mm x 8-mm-i.d. column using a solvent gradient composed of a poor solvent such as water, methanol, or acetonitrile and increasing amounts of a good solvent, tetrahydrofuran. Excellent separations are obtained, demonstrating that precipitation-redissolution can be a suitable alternate to size exclusion chromatography (SEC) of some polymers. Compared to SEC, the gradient elution separation can be achieved at higher flow rates in a much shorter time. Precipitation-redissolution with gradient elution can also be used for the separation of copolymers, for which the process is controlled not only by molecular weight but also by the composition of the copolymers.

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Section: Resultsmentioning

confidence: 99%

Molded Monolithic Rod of Macroporous Poly(styrene-co-divinylbenzene) as a Separation Medium for HPLC of Synthetic Polymers: “On-Column” Precipitation−Redissolution Chromatography as an Alternative to Size Exclusion Chromatography of Styrene Oligomers and Polymers

et al. 1996

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“…7,11,18,36,37 Our early work with styrene oligomers demonstrated that this effect was much less evident for separations involving monolithic columns. 33 Figure 8 shows the separations of five polystyrene standards injected in solutions with different overall concentrations ranging from 2.5 to 50 mg/mL.…”

Section: Sample Loadmentioning

confidence: 99%

Rapid determination of molecular parameters of synthetic polymers by precipitation/redissolution high-performance liquid chromatography using ?molded? monolithic column

Skora

Švec

et al. 2000

J. Polym. Sci. A Polym. Chem.

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“…The comparison of the retention behavior of complex macromolecules of special interactions and architecture features with that of linear polymers is often employed. Unless the effects of microscopic parameters are determined, such comparisons and the use of calibration curves of linear chains to derive results for chains of other architectures are not always understandable and accurate …”

Section: Introductionmentioning

confidence: 99%

Effects of Substrate Interactions in Liquid Chromatography of Star Homopolymers and Star Block Copolymers

2001

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A theoretical study of the behavior in liquid chromatography of star polymers of two different kinds of arms is presented, including the special cases of diblock copolymers and regular star polymers. The two different kinds of interactions between the substrate and the monomeric units which can be repulsive attractive or neutral combine in these heteroarm stars. They can act cooperatively or antagonistically determining, in connection with the effects of the architecture and the sizes of the branches, the mode of retention of the macromolecules. The compensation point, where no surface effects remain when the surface−monomer interactions are negligible, can also be reached by cancellation of the opposite but not negligible interactions of the two different kinds of arms with the substrate.

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Sample size and retention values in high-performance liquid chromatography of biological and synthetic polymers

Cited by 18 publications

References 14 publications

Molded Monolithic Rod of Macroporous Poly(styrene-co-divinylbenzene) as a Separation Medium for HPLC of Synthetic Polymers: “On-Column” Precipitation−Redissolution Chromatography as an Alternative to Size Exclusion Chromatography of Styrene Oligomers and Polymers

Molded Monolithic Rod of Macroporous Poly(styrene-co-divinylbenzene) as a Separation Medium for HPLC of Synthetic Polymers: “On-Column” Precipitation−Redissolution Chromatography as an Alternative to Size Exclusion Chromatography of Styrene Oligomers and Polymers

Rapid determination of molecular parameters of synthetic polymers by precipitation/redissolution high-performance liquid chromatography using ?molded? monolithic column

Effects of Substrate Interactions in Liquid Chromatography of Star Homopolymers and Star Block Copolymers

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