Abstract. We followed the development of a Pseudomonas fluorescens biofilm on quartz sand in an instrumented horizontal flow-through stainless steel container (SSC) in lysimeter-scale, to investigate its impact on physical soil parameters within the aquifer and the capillary fringe (CF). Increasingly concentrated biodegradable substances in a liquid medium were pumped through the sand in the SSC creating an artificial aquifer. Biofilm development and its influence on flow parameters and oxygen concentration profiles, as well as temperature or water suction changes with time were determined. After more than 19 weeks of medium flow, at DOC concentrations of finally about 300 mg/L, P. fluorescens formed a strong biofilm and the highest biomass concentrations with up to 2.8 mg volatile solids per g dry sand were found in the transition zone of the CF. The soil temperature, which was slightly increased, or the water suction was significantly influenced during the experiment. The effects were strongest within the first 0.3 m of flow stretch, where the highest biomass values were detected. During the total experimental duration of 54 weeks the average flow velocity was reduced by up to 15 % due to clogging effects and the oxygen profiles were changed drastically. Results may be representative for real polluted aquifers.
Keywords:Pseudomonas fluorescens, sand aquifer, capillary fringe, soil contamination, biofilm formation, biological clogging.
IntroductionSoil and groundwater are naturally inhabited by a vast variety of prokaryotes. It was estimated that 2.5 x 10 29 microorganisms live in the top 8 m of the terrestrial subsurface and at least 2.5 x 10 30 microorganisms below 8 m depth, either suspended in the water phase or attached onto organic and mineral compounds of top soil and the underground [53]. Anaerobic bacteria in deep soil layers may ferment organic pollutants to mainly fatty acids whereas aerobic bacteria in upper soil layers may respire organic pollutants in the presence of oxygen to carbon dioxide and thus contribute to "clean-up". There seems to be a spatial heterogeneity of microorganisms. Whereas mainly aerobic bacteria, fungi and protozoa colonize soil surface layers, the proportion of mainly anaerobic bacteria (most of them are "fermenters") and of Actinomycetes increases with depth in the underground in the vicinity and below the groundwater table. These population changes are accompanied by a significantly decreasing diversity of microorganisms [17] and by a decrease of physiological functions [45]. Groundwater bacteria tend to form a biofilm on mineral surfaces of soil particles and may cause preferential flow paths or clogging in heterogeneous soil compartments [45]. In contrast aerobic motile bacteria can even move into the top zone of the capillary fringe (CF, spanning from the groundwater table to still visible moisture above it in a quartz sand aquifer) above the water-saturated zone of quartz sand and form a dense biofilm in the still highly saturated interface region of the CF [31]. Biofi...