Sandblasted-acid-etched titanium surface influences in vitro the biologicalbehavior of SaOS-2 human osteoblast-like cells

Abstract

Osseointegrated dental implants have been successfully used over the past several years, allowing functional replacement of missing teeth. Surface properties of titanium dental implants influence bone cell response. Implant topography appears to modulate cell growth and differentiation of osteoblasts thus affecting the bone healing process. Optimal roughness and superficial morphology are still controversial and need to be clearly defined. In the present study we evaluated in vitro the biological behavior of SaOS-2 cells, a human osteoblast-like cell line, cultured on two different titanium surfaces, smooth and sandblasted-acid-etched, by investigating cell morphology, adhesion, proliferation, expression of some bone differentiation markers and extracellular matrix components. Results showed that the surface topography may influence in vitro the phenotypical expression of human osteoblast-like cells. In particular the tested sandblasted-acid-etched titanium surface induced a significantly increased Co I deposition and 2-β1 receptor expression as compared to the relatively smooth surface, promoting a probable tendency of SaOS-2 cells to shift toward a mature osteoblastic phenotype. It is therefore likely that specific surface properties of sandblasted-acid-etched titanium implants may modulate the biological behavior of osteoblasts during bone tissue healing.

The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

“…Saos-2 cells were used for studying cell-material interaction on Ti, Nb, and TiNb alloy with different surface morphology, topography, roughness, and charge distribution. [36][37][38][39] Osteogenic differentiation markers reach maximum level after 7 or 14 days in vitro. 37 For tissue-engineering purposes, it is important to study osteogenic differentiation at shorter time intervals, as early onset of osteogenic differentiation and bone formation on a biomaterial surface is crucial for successful implantation in vivo.…”

Osteoblast Response to Different UVA‐Activated Anatase Implant Coatings

“…Since these surfaces have not been “activated” by UVA, these findings must be related to the influence of microtopography and additional submicrotopography. Other researchers have also found that a sandblasted‐acid‐etched titanium surface can induce a significantly increased CoI deposition and 2‐β1 receptor expression as compared to a relatively smooth surface, promoting a tendency of Saos‐2 cells to shift toward a mature osteoblastic phenotype. In a systematic review on effects of surface topography, Wennerberg and Albrektsson has suggested that titanium implants with an average roughness of S a of 1–2 µm can improve bone‐to‐implant contact.…”

Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells

“…These surface effects were also observed in other studies showing, osteoblasts the etched surface of titanium promoted high Co I production and increased level of α2-β1 receptor. [35][36][37] In this regard, future work should be designed to introduce optimization of surface roughness in hydrogels to create a favorable microenvironment for gingival cells.…”