Sauchinone Attenuates Oxidative Stress-Induced Skeletal Muscle Myoblast Damage through the Down-Regulation of Ceramide

Abstract

We investigated the effects of sauchinone, isolated from the root of Saururus chinensis, on muscle disorders and the underlying mechanism of oxidative stress-induced C 2 C 12 skeletal muscle myoblast damage. To assess the protective effects of sauchinone on oxidative stress-induced C 2 C 12 skeletal muscle myoblasts, we measured the viability of the cells, showing that sauchinone pre-treatment significantly reduced the decreased cell viability after H 2 O 2 treatment. We also investigated the mechanism of this protective effect of sauchinone. In Western blot analysis, the heat shock protein (HSP)-70 level increased significantly in the sauchinone-pretreated myoblasts. We used high performance liquid chromatography (HPLC) to examine the level of endogenous ceramide after pre-treatment with sauchinone followed by exposure to H 2 O 2 . While hydrogen peroxide increased the ceramide content to approximately 166.60؎ ؎38.93% of the control level, pre-treatment with sauchinone inhibited this increase, maintaining the ceramide content at the control level. We demonstrated that sauchinone regulates intracellular HSP70 expression as well as ceramide levels to protect against oxidative stress-induced C 2 C 12 muscle myoblast damage. We suggest the potential benefits of herbal medicines in the treatment of oxidative stressrelated muscle disorders.

Celastrus paniculatus Willd. mitigates t-BHP induced oxidative and apoptotic damage in C2C12 murine muscle cells

“…#P \ 0.01 versus control group, *P \ 0.01 versus control cells and *P \ 0.01 versus 250 lM t-BHP treated group aggregated proteins and induces proteolytic degradation of denaturated proteins (Gupta et al 2010). Increased expression of HSP-70 has been reported in radical induced oxidative stress condition (Jung et al 2011;Kumar and Khanum 2013). The protective effect of C. paniculatus against t-BHP induced HSP-70 down-regulation further confirms the anti-stress effects.…”

Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-κB and AP-1 inactivation in LPS-induced RAW264.7 cells

“…The isolated lignans from S. chinensis and S. cernuus showed potential anti-inflammatory, anti-metastatic, neuroleptic, and cholesterol-lowering activities [15,16,[28][29][30][31][32][33]. Additionally, these compounds exhibited hepatoprotective, antifibrotic, antiseptic, antioxidative, anti-tumor DNA replication, and anti-HIV effects [2,4,[34][35][36]. Therefore, the Saururus species of plants may be useful in human diseases.…”

Modulating exercise-induced hormesis: Does less equal more?

“…HSF-1 and HSPs may assist muscle 352 regeneration by protecting muscle cells against oxidative damage, apoptosis, and ATP 353 depletion (16,(87)(88)(89)118). HSPs may also promote repair of muscle tissue by activating the 354 signaling pathways involved in protein synthesis (e.g., Akt, p70S6 kinase, and ERK) (61) and 355 by regulating the activity of enzymes and transcription factors that can cause degeneration 356 and/or atrophy of muscle fibers (38,49,105,195).…”