SBF assays, direct and indirect cell culture tests to evaluate the biological performance of bioglasses and bioglass-based composites: Three paradigmatic cases

Bellucci, Devis; Salvatori, Roberta; Anesi, Alexandre; Chiarini, Luigi; Cannillo, Valeria

doi:10.1016/j.msec.2018.12.006

Cited by 46 publications

(36 citation statements)

References 54 publications

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“…The reason behind the drop-in performance in the long term (72 h) and in the second use group of assays based on eluate (BrdU and MTT) needs to be argued. Important discrepancies should be expected between the findings of the direct culture tests and those arising from the indirect ones [29]. To this aim, the materials' cytotoxicity has been investigated both through direct contact tests-where the cells are seeded directly onto the material and then incubated in suitable conditions-and indirect contact (eluate), in order to observe the possible negative effects of the samples' eluates.…”

Section: Resultsmentioning

confidence: 99%

“…All samples underwent both direct (NR uptake) and indirect (extract, eluate) contacts for the cytocompatibility assays (MTT and BrdU, see below) [29].…”

Section: Assessment Of Cytocompatibilitymentioning

confidence: 99%

“…Its first clinical use in an implant was aimed to replace small bones in the middle ear to treat conductive hearing loss [27]. Since then, the possible applications in the biomedical field have been widely studied [28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

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Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

et al. 2020

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(1) Background: a cell evaluation focused to verify the self-regenerative antioxidant activity is performed on cerium doped bioactive glasses. (2) Methods: the glasses based on 45S5 Bioglass®, are doped with 1.2 mol%, 3.6 mol% and 5.3 mol% of CeO2 and possess a polyhedral shape (~500 µm2). Glasses with this composition inhibit oxidative stress by mimicking catalase enzyme (CAT) and superoxide dismutase (SOD) activities; moreover, our previous cytocompatibility tests (neutral red (NR), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Bromo-2-deoxyUridine (BrdU)) reveal that the presence of cerium promotes the absorption and vitality of the cells. The same cytocompatibility tests were performed and repeated, in two different periods (named first and second use), separated from each other by four months. (3) Results: in the first and second use, NR tests indicate that the presence of cerium promotes once again cell uptake and viability, especially after 72 h. A decrease in cell proliferation it is observed after MTT and BrdU tests only in the second use. These findings are supported by statistically significant results (4) Conclusions: these glasses show enhanced proliferation, both in the short and in the long term, and for the first time such large dimensions are studied for this kind of study. A future prospective is the implantation of these bioactive glasses as bone substitute in animal models.

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Section: Resultsmentioning

confidence: 99%

“…All samples underwent both direct (NR uptake) and indirect (extract, eluate) contacts for the cytocompatibility assays (MTT and BrdU, see below) [29].…”

Section: Assessment Of Cytocompatibilitymentioning

confidence: 99%

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Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

et al. 2020

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“…Generally, bioactive glasses have shown high bioactivity and high interaction with hard and soft tissues [14,15]. In this work, 45S5 BG (composition 45 wt% SiO 2 , 24.5 wt% CaO, 24.5 wt% Na 2 O, and 6.0 wt% P 2 O 5 ) [16], Sr-and Mg-substituted bioactive glass (BGMS10 [17][18][19][20][21][22][23]), and Zn-substituted bioactive glass (BGMS_2Zn [24]) were incorporated in PCL fibers by electrospinning technique. BGMS10 was designed starting from the composition of 45S5 by adding SrO and MgO; subsequently, ZnO was added to the BGMS10 composition to produce a Zn-substituted bioactive glass labelled as BGMS_2Zn [24].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Bioactive Glasses Containing Mg, Sr, and Zn in Electrospun PCL Fibers by Using Benign Solvents

et al. 2020

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Poly(ε-caprolactone) (PCL) and PCL/bioactive glass composite fiber mats were produced by electrospinning technique. To improve cell adhesion and proliferation (i) 45S5, (ii) a bioactive glass containing strontium and magnesium oxides, and (iii) a bioactive glass containing zinc oxide were separately added to the starting PCL solution before electrospinning. A good incorporation of bioactive glass particles in PCL electrospun mats was confirmed by SEM and FTIR analyses. Bioactivity was evaluated by immersion of PCL mats and PCL/bioactive glass electrospun fiber mats in simulated body fluid (SBF). Bone murine stromal cells (ST-2) were employed in WST-8 assay to assess cell viability, cell morphology, and proliferation. The results showed that the presence of bioactive glass particles in the fibers enhances cell adhesion and proliferation compared to neat PCL mats. Furthermore, PCL/bioactive glass electrospun mats showed higher wound-healing rate (measured as cell migration rate) in vitro compared to neat PCL electrospun mats. Therefore, the characteristics of the PCL matrix combined with biological properties of bioactive glasses make PCL/bioactive glass composite ideal candidate for biomedical application.

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“…Moreover, the addition of therapeutic ions such as strontium and magnesium resulted in an improved biological response [ 12 ] also compared to 45S5 [ 13 ], as reported as well for other bioactive glasses containing Sr and Mg [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. BGMS10 resulted as suitable for the preparation of composites [ 21 ], dental putties [ 22 ], 3D printed scaffolds [ 23 ], wound dressings [ 24 , 25 ], and antibacterial systems [ 26 ], being very promising also compared to other bioactive glasses investigated in literature (e.g., [ 27 , 28 , 29 , 30 , 31 , 32 ]).…”

Section: Introductionmentioning

confidence: 99%

Effects of a Novel Bioactive Glass Composition on Biological Properties of Human Dental Pulp Stem Cells

et al. 2020

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Functional reconstruction of bone defects represents a clinical challenge in the regenerative medicine field, which targets tissue repair following traumatic injuries and disease-related bone deficiencies. In this regard, the optimal biomaterial should be safe, biocompatible and tailored in order to promote the activation of host progenitor cells towards bone repair. Bioactive glasses might be suitable biomaterials due to their composition being able to induce the host healing response and, eventually, anti-bacterial properties. In this study we investigated whether and how an innovative bioactive glass composition, called BGMS10, may affect cell adhesion, morphology, proliferation, immunomodulation and osteogenic differentiation of human dental pulp stem cells (hDPSCs). When cultured on BGMS10, hDPSCs maintained their proliferation rate and typical fibroblast-like morphology, showing the expression of stemness markers STRO-1 and c-Kit. Moreover, the expression of FasL, a key molecule in mediating immunomodulation effects of hDPSCs, was maintained. BGMS10 also proved to trigger osteogenic commitment of hDPSCs, as confirmed by the activation of bone-related transcription factors RUNX2 and Osx and the ongoing deposition of extracellular matrix supported by the expression of OPN and OCN. Our findings suggest that BGMS10 not only maintains the typical biological and immunomodulatory properties of hDPSCs but also favors the osteogenic commitment.

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SBF assays, direct and indirect cell culture tests to evaluate the biological performance of bioglasses and bioglass-based composites: Three paradigmatic cases

Cited by 46 publications

References 54 publications

Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

Incorporation of Bioactive Glasses Containing Mg, Sr, and Zn in Electrospun PCL Fibers by Using Benign Solvents

Effects of a Novel Bioactive Glass Composition on Biological Properties of Human Dental Pulp Stem Cells

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