Scaffold design for artificial tissue with bone marrow stem cells

Noreikaitė, Aurelija; Antanavičiūtė, Ieva; Mikalayeva, Valeryia; Darinskas, Adas; Tamulevičius, Tomas; Adomavičiūtė, Erika; Šimatonis, Linas; Akramienė, Dalia; Stankevičius, Edgaras

doi:10.1016/j.medici.2017.07.001

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…They can provide irregular and rough surface, which is conducive to cell adhesion and proliferation, and is sought after. 54 , 73 …”

Section: Drugs Delivered In Bone Tissue Engineering Composite Scaffoldsmentioning

confidence: 99%

“…They can provide irregular and rough surface, which is conducive to cell adhesion and proliferation, and is sought after. 54,73 MicroRNAs (miRNA) are a kind of noncoding single-stranded RNA molecules with a length of about 22 nucleotides encoded by endogenous genes, which are involved in the regulation of post-transcriptional gene expression in bone tissues. In recent years, the use of microRNAs to induce osteogenesis and promote neovascularization of osteoblasts is expected to become an alternative approach for bone tissue engineering.…”

Section: Stem Cells and Micrornamentioning

confidence: 99%

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Research Progress of Design Drugs and Composite Biomaterials in Bone Tissue Engineering

Guo¹,

Song²,

Lu³

et al. 2023

IJN

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Bone, like most organs, has the ability to heal naturally and can be repaired slowly when it is slightly injured. However, in the case of bone defects caused by diseases or large shocks, surgical intervention and treatment of bone substitutes are needed, and drugs are actively matched to promote osteogenesis or prevent infection. Oral administration or injection for systemic therapy is a common way of administration in clinic, although it is not suitable for the long treatment cycle of bone tissue, and the drugs cannot exert the greatest effect or even produce toxic and side effects. In order to solve this problem, the structure or carrier simulating natural bone tissue is constructed to control the loading or release of the preparation with osteogenic potential, thus accelerating the repair of bone defect. Bioactive materials provide potential advantages for bone tissue regeneration, such as physical support, cell coverage and growth factors. In this review, we discuss the application of bone scaffolds with different structural characteristics made of polymers, ceramics and other composite materials in bone regeneration engineering and drug release, and look forward to its prospect.

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“…They can provide irregular and rough surface, which is conducive to cell adhesion and proliferation, and is sought after. 54 , 73 …”

Section: Drugs Delivered In Bone Tissue Engineering Composite Scaffoldsmentioning

confidence: 99%

Section: Stem Cells and Micrornamentioning

confidence: 99%

Research Progress of Design Drugs and Composite Biomaterials in Bone Tissue Engineering

Guo¹,

Song²,

Lu³

et al. 2023

IJN

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“…Various studies have successfully applied 3D printed scaffolds in promoting chondrogenesis of bone mesenchymal stem cells (BMSCs) [2,3]. Further studies improved the precise and accurate 3D structures for cartilage tissue engineering [4].…”

Section: Introductionmentioning

confidence: 99%

Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration

Yao

et al. 2019

Med Sci Monit

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Background We synthetized a 3D printed poly-ɛ-caprolactone (PCL) scaffold with polydopamine (PDA) coating and lithium chloride (LiCl) deposition for cartilage tissue engineering and analyzed its effect on promoting rabbit bone marrow mesenchymal stem cells (rBMSC) chondrogenesis in vitro . Material/Methods PCL scaffolds were prepared by 3D printing with a well-designed CAD digital model, then modified by PDA coating to produce PCL-PDA scaffolds. Finally, LiCl was deposited on the PDA coating to produce PCL-PDA-Li scaffolds. The physicochemical properties, bioactivity, and biocompatibility of PCL-PDA-Li scaffolds were accessed by comparing them with PCL scaffolds and PCL-PDA scaffolds. Results 3D PCL scaffolds exhibited excellent mechanical integrity as designed. PDA coating and LiCl deposition improved surface hydrophilicity without sacrificing mechanical strength. Li + release was durable and ion concentration did not reach the cytotoxicity level. This in vitro study showed that, compared to PCL scaffolds, PCL-PDA and PCL-PDA-Li scaffolds significantly increased glycosaminoglycan (GAG) formation and chondrogenic marker gene expression, while PCL-PDA-Li scaffolds showed far higher rBMSC viability and chondrogenesis. Conclusions 3D printed PCL-PDA-Li scaffolds promoted chondrogenesis in vitro and may provide a good method for lithium administration and be a potential candidate for cartilage tissue engineering.

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“…Therefore, 3D printed PLA scaffolds can enrich BCSC niche in a MDA-MB-231 cell line in less time than other types of scaffolds can. Noreikaité et al demonstrated that PLA electrospun scaffolds ensured a suitable environment for cell growth with mesenchymal stem cells which were capable of spreading after three days of cultivation [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion

Polonio‐Alcalá

Rabionet

Guerra

et al. 2018

IJMS

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Breast cancer stem cells (BCSCs) are tumor-initiating cells responsible for metastasis and tumor reappearance, but their research is limited by the impossibility to cultivate them in a monolayer culture. Scaffolds are three-dimensional (3D) cell culture systems which avoid problems related with culturing BCSC. However, a standardized scaffold for enhancing a BCSC population is still an open issue. The main aim of this study is to establish a suitable poly (lactic acid) (PLA) scaffold which will produce BCSC enrichment, thus allowing them to be studied. Different 3D printing parameters were analyzed using Taguchi experimental design methods. Several PLA scaffold architectures were manufactured using a Fused Filament Fabrication (FFF) 3D printer. They were then evaluated by cell proliferation assay and the configurations with the highest growth rates were subjected to BCSC quantification by ALDH activity. The design SS1 (0.2 mm layer height, 70% infill density, Zigzag infill pattern, 45° infill direction, and 100% flow) obtained the highest proliferation rate and was capable of enhancing a ALDH+ cell population compared to 2D cell culture. In conclusion, the data obtained endorse the PLA porous scaffold as useful for culturing breast cancer cells in a microenvironment similar to in vivo and increasing the numbers of BCSCs.

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Scaffold design for artificial tissue with bone marrow stem cells

Cited by 6 publications

References 30 publications

Research Progress of Design Drugs and Composite Biomaterials in Bone Tissue Engineering

Research Progress of Design Drugs and Composite Biomaterials in Bone Tissue Engineering

Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration

Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion

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