Scale-up of membrane fixed enzyme reactors: modelling and experiments

Prenosil, J. E.; Hediger, T.

doi:10.1016/0011-9164(85)85066-9

Cited by 10 publications

(2 citation statements)

References 3 publications

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“…In addition to operational (back pressure, aggregation, clogging) and economical (expensive) disadvantages, commonly noted diffusion limitations in these immobilized systems not only reduce the reaction rate in general but also affect the product spectrum and specifically reduce GOS formation. For example, 20-30% decreases in the GOS formation has been reported with immobilized enzymes due to introduction of mass transfer resistance in the system (28)(29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 97%

Immobilization of β‐Galactosidase on Fibrous Matrix by Polyethyleneimine for Production of Galacto‐Oligosaccharides from Lactose

Albayrak

Yang

2002

Biotechnology Progress

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The production of galacto-oligosaccharides (GOS) from lactose by Aspergillus oryzae beta-galactosidase immobilized on cotton cloth was studied. A novel method of enzyme immobilization involving PEI-enzyme aggregate formation and growth of aggregates on individual fibrils of cotton cloth leading to multilayer immobilization of the enzyme was developed. A large amount of enzyme was immobilized (250 mg/g support) with about 90-95% efficiency. A maximum GOS production of 25-26% (w/w) was achieved at near 50% lactose conversion from 400 g/L of lactose at pH 4.5 and 40 degrees C. Tri- and tetrasaccharides were the major types of GOS formed, accounting for about 70% and 25% of the total GOS produced in the reactions, respectively. Temperature and pH affected not only the reaction rate but also GOS yield to some extend. A reaction pH of 6.0 increased GOS yield by as much as 10% compared with that of pH 4.5 while decreased the reaction rate of immobilized enzyme. The cotton cloth as the support matrix for enzyme immobilization did not affect the GOS formation characteristics of the enzyme under the same reaction conditions, suggesting diffusion limitation was negligible in the packed bed reactor and the enzyme carrier. Increase in the thermal stability of PEI-immobilized enzyme was also observed. The half-life for the immobilized enzyme on cotton cloth was close to 1 year at 40 degrees C and 21 days at 50 degrees C. Stable, continuous operation in a plug-flow reactor was demonstrated for about 3 days without any apparent problem. A maximum GOS production of 26% (w/w) of total sugars was attained at 50% lactose conversion with a feed containing 400 g/L of lactose at pH 4.5 and 40 degrees C. The corresponding reactor productivity was 6 kg/L/h, which is several-hundred-fold higher than those previously reported.

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Section: Introductionmentioning

confidence: 97%

Immobilization of β‐Galactosidase on Fibrous Matrix by Polyethyleneimine for Production of Galacto‐Oligosaccharides from Lactose

Albayrak

Yang

2002

Biotechnology Progress

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“…Using this mode, DAVIS loaded hollow fiber dialyzer, manufactured by DOW CHEMICAL COMPANY, with alkaline phosphatase [16]. PRENOSIL and HEDIGER immobilized 8-galactosidase in a backflush mode using laboratory and industrial size cartridges [17]. It turned out that the module size did not have any influence on the final specific activity per the membrane ares unit.…”

Section: Immobilization Of Enzymes and Cellsmentioning

confidence: 99%

Hollow fiber modules as a tool for enzyme and cell immobilization

Rucka

Sroka

1989

Acta Biotechnologica

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Formation of oligosaccharides during enzymatic lactose hydrolysis and their importance in a whey hydrolysis process: Part II: Experimental

Prenosil

Stuker

Bourne

1987

Biotech & Bioengineering

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Enzymatic lactose hydrolysis using two yeast and two fungal lactases that are of current technical interest was studied. The enzymes were compared regarding their oligosaccharide production. Parameters influencing oligosaccharide formation, together with the effect of immobilization were examined and conditions minimizing oligosaccharide content in the hydrolysis product were proposed. Enzymatic whey hydrolysis was also considered. A possibility of enzymatic lactose recombination from its hydrolysis products was shown.

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Scale-up of membrane fixed enzyme reactors: modelling and experiments

Cited by 10 publications

References 3 publications

Immobilization of β‐Galactosidase on Fibrous Matrix by Polyethyleneimine for Production of Galacto‐Oligosaccharides from Lactose

Immobilization of β‐Galactosidase on Fibrous Matrix by Polyethyleneimine for Production of Galacto‐Oligosaccharides from Lactose

Hollow fiber modules as a tool for enzyme and cell immobilization

Formation of oligosaccharides during enzymatic lactose hydrolysis and their importance in a whey hydrolysis process: Part II: Experimental

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