Scanning electron microscopy and microbiological approaches for the evaluation of salivary microorganisms behaviour on anatase titanium surfaces: In vitro study

Bernardi, Sara; Bianchi, Serena; Botticelli, Gianluca; Rastelli, E.; Tomei, Anna Rita; Palmerini, Maria Grazia; Continenza, Maria Adelaide; Macchiarelli, Guido

doi:10.1016/j.morpho.2017.12.001

Cited by 23 publications

(21 citation statements)

References 20 publications

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“…Based on the initial proposed hypothesis, both implant macrogeometries were prepared with and without (machined only) surface treatment. The surface treatment used was performed by blasting with microparticles (~100 µm) of titanium oxide and followed by application of maleic acid, showing a roughness with Ra = 0.56 ± 0.10 µm [8]. Figure 3 shows the scanning electronic microscopy (SEM) of the two surfaces used for the comparison.…”

Section: Methodsmentioning

confidence: 99%

“…Several investigations to improve or accelerate the process of osseointegration have been studied, as well worked on to elaborate new treatments for the surface of the implants (micro topography) with different physical and chemical characteristics [5][6][7][8][9]. These modifications have shown good results, mainly in pre-clinical studies, as reported in the literature [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

et al. 2019

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A new implant design with healing chambers in the threads was analyzed and compared with a conventional implant macrogeometry, both implants models with and without surface treatment. Eighty conical implants were prepared using commercially pure titanium (grade IV) by the company Implacil De Bortoli (São Paulo, Brazil). Four groups were performed, as described below: Group 1 (G1), traditional conical implants with surface treatment; group 2 (G2), traditional conical implants without surface treatment (machined surface); group 3 (G3), new conical implant design with surface treatment; group 4 (G4), new conical implant design without surface treatment. The implants were placed in the two tibias (n = 2 implants per tibia) of twenty New Zealand rabbits determined by randomization. The animals were euthanized after 15 days (Time 1) and 30 days (Time 2). The parameters evaluated were the implant stability quotient (ISQ), removal torque values (RTv), and histomorphometric evaluation to determine the bone to implant contact (%BIC) and bone area fraction occupancy (BAFO%). The results showed that the implants with the macrogeometry modified with healing chambers in the threads produced a significant enhancement in the osseointegration, accelerating this process. The statistical analyses of ISQ and RTv showed a significative statistical difference between the groups in both time periods of evaluation (p ≤ 0.0001). Moreover, an important increase in the histological parameters were found for groups G3 and G4, with significant statistical differences to the BIC% (in the Time 1 p = 0.0406 and in the Time 2 p < 0.0001) and the BAFO% ((in the Time 1 p = 0.0002 and in the Time 2 p = 0.0045). In conclusion, the result data showed that the implants with the new macrogeometry, presenting the healing chambers in the threads, produced a significant enhancement in the osseointegration, accelerating the process.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

et al. 2019

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“…It is etiologically related to the microbiota of the dorsal tongue biofilm (Roldán, Herrera, & Sanz, ; Yaegaki & Coil, ), and in particular to the presence of anaerobic microorganisms responsible for the production of VSCs, such as Centipeda periodontii, Eikenella corrodens , Fusobacterium nucleatum, F. periodonticum , Porphyromonas gingivalis , Prevotella melaninogenica , P. intermedia , Solobacterium moorei , Tannerella forsythia, and Treponema denticola. Due to its papillary structure that creates an ecological niche for microorganisms, the tongue biofilm represents an oral microenvironment which is well distinguished from the supragingival biofilm, also known as dental plaque, and the subgingival biofilm (Amou, Hinode, Yoshioka, & Grenier, ; Bernardi et al, ; Bernardi, Marzo, & Continenza, ; Bernardi, Zeka, Mummolo, Marzo, & Continenza, ).…”

Section: Introductionmentioning

confidence: 99%

Combining culture and culture‐independent methods reveals new microbial composition of halitosis patients' tongue biofilm

Bernardi

Karygianni

Filippi³

et al. 2019

MicrobiologyOpen

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractBackground: Oral malodor is a very discomforting condition deriving from the presence of volatile sulfur compounds in the expired air. In halitosis of intraoral etiology, the volatile sulfur compounds are metabolic products of the oral microorganisms within the biofilm coating the tongue dorsum as well as other tissues in the oral cavity. The aim of this study was to characterize and compare the microbial composition of tongue biofilm in volunteers suffering from halitosis and healthy volunteers by means of both the culture method and culture-independent cloning technique.Results: A high bacterial variety (more than 80 different species) was detected using the combination of both methods. A distinct bacterial composition was revealed in the halitosis-associated biofilms compared with the health-associated biofilms.Actinomyces graevenitzii was shown to be significantly associated with the halitosis condition. The culture method identified 47 species, included Veillonella rogosae, never isolated from the tongue biofilm of halitosis patients so far. In the healthy condition, the culture-dependent method showed that the most frequent species were Streptococcus parasanguinis among the aerobes and Veillonella spp. among the anaerobes. The culture-independent cloning method detected more than 50 species.Streptococci, in particular S. mitis/oralis, S. pseudopneumoniae, and S. infantis as well as Prevotella spp., were found most frequently in halitosis patients. Streptococcus salivarius and Rothia mucilaginosa were found more frequently in the healthy condition. Conclusions:The combination of the culture-dependent and culture-independent cloning techniques allowed for a widespread analysis of the tongue biofilm in halitosis patients. The results can support further pharmacological research for new antimicrobial agents and halitosis therapy strategies. K E Y W O R D Sculture-independent cloning technique, halitosis, microbial culture, tongue biofilm | 11 of 12

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“…Hair dyes are divided into two categories: synthetic (most used category, with a longer life) and natural (such as those derived from the leaves of Lawsonia inermis) (Guerra-Tapia & Gonzalez-Guerra). A recent study demonstrated that 64 % of French women rely on the use of scalp hair colors and among them only the 2.64 % uses dyes, based on henna powder (Bernard et al, 2016). However, the synthetic dyes affect the hair structure significantly enhancing the oxidation of melanin and any dyes previously applied; this process creates a gap on the hair cortex, allowing the deep penetration of the chromophores (Draelos; Guerra-Tapia & Gonzalez-Guerra, 2014).…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

et al. 2020

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During aging, usually graying of the hair occurs as a result of oxidative stress. Driven by social acceptance and self-perception of the exterior appearance, both men and women rely on hair dyeing products, in order to mask the graying hair. At the same time, a frequent use of synthetic products and treatment can damage the hair shaft; for this reason, this study aimed to evaluate the morphological effect of the herbal dye derived from Lawsonia inermis (henna), on hair. Dyed hairs were evaluated by means of SEM. Subsequently, they were compared, qualitatively and quantitatively, with undyed hairs. Results showed a positive impact on the cuticula pattern and on the diameters of the examined samples, after henna application. Different results, about the degree and the type of morphological changes occurring on pigmented hairs, may depend on the phenotype and on the health condition of hair, before dye treatment.

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Scanning electron microscopy and microbiological approaches for the evaluation of salivary microorganisms behaviour on anatase titanium surfaces: In vitro study

Cited by 23 publications

References 20 publications

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

New Implant Macrogeometry to Improve and Accelerate the Osseointegration: An In Vivo Experimental Study

Combining culture and culture‐independent methods reveals new microbial composition of halitosis patients' tongue biofilm

Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

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