Scanning electron microscopy of bacteria <i>Tetrasphaera duodecadis</i>

Arroyo, Elsa; Enríquez, Lorenzo Jarquín; Sanchez, A. Portera; Ovalle, Marcela; Olivas, A.

doi:10.1002/sca.21154

Cited by 15 publications

(10 citation statements)

References 13 publications

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“…Bacterial endophytes LB‐132 taken from the NA medium were fixed with glutaraldehyde 2.5% (v/v) in a 0.1 M phosphate buffer solution (PBS, pH 7.2), for 24 h at 4 °C; and then dehydrated by 10%, 30%, 50%, 70%, 85%, 95% and 100% of ethanol (Song et al ., 2017). Scanning electron microscopy (SEM, SU8010, Hitachi, Japan) at 15.0 kV was used to observe the morphology of bacterial strains (Arroyo et al ., 2014). Fungal endophytes SF‐85 and RF‐1 taken from the PDA medium were noted, the conidia and conidial head of fungal isolates were observed with microscope (Smart, China) by lactophenol cotton blue stain (Zheng et al ., 2017).…”

Section: Methodsmentioning

confidence: 99%

Endophytes isolated from Panax notoginseng converted ginsenosides

Wei

Chen

Wang

et al. 2021

Microbial Biotechnology

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Endophytes may participate in the conversion of metabolites within medicinal plants, influencing the efficacy of host. However, the distribution of endophytes within medicinal plants P. notoginseng and how it contributes to the conversion of saponins are not well understood. Here, we determined the distribution of saponins and endophytes within P. notoginseng compartments and further confirm the saponin conversion by endophytes. We found metabolites showed compartment specificity within P. notoginseng. Potential saponin biomarkers, such as Rb1, Rg1, Re, Rc and Rd, were obtained. Endophytic diversity, composition and co-occurrence networks also showed compartment specificity, and bacterial alpha diversity values were highest in root compartment, consistently decreased in the stem and leaf compartments, whereas those of fungi showed the opposite trend. Potential bacterial biomarkers, such as Rhizobium, Bacillus, Pseudomonas, Enterobacter, Klebsiella, Pantoea and fungal biomarkers Phoma, Epicoccum, Xylariales, were also obtained. Endophytes related to saponin contents were found by Spearman correlation analysis, and further verification experiments showed that Enterobacter chengduensis could convert ginsenoside Rg1 to F1 at a rate of 13.24%; Trichoderma koningii could convert ginsenoside Rb1 to Rd at a rate of 40.00% and to Rg3 at a rate of 32.31%; Penicillium chermesinum could convert ginsenoside Rb1 to Rd at a rate of 74.24%.

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Section: Methodsmentioning

confidence: 99%

Endophytes isolated from Panax notoginseng converted ginsenosides

Wei

Chen

Wang

et al. 2021

Microbial Biotechnology

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“…Scanning electron microscopy was performed as described by Arroyo et al . 57 with slight modifications using the scanning electron microscope (SEM) (EFI Quanta 200 3D, USA). For biochemical characterization, various tests were conducted including carbohydrate fermentation (glucose, lactose, sucrose, mannitol, maltose, raffinose, sorbitol and starch), esculin and starch hydrolysis, indole, lysine decarboxylase, catalase and oxidase production, and Voges-Proskauer and methyl red tests.…”

Section: Methodsmentioning

confidence: 99%

Partial rpoB Gene Sequencing Identification and Probiotic Potential of Floricoccus penangensis ML061-4 Isolated from Assam Tea (Camellia sinensis var. assamica)

Rungsirivanich

Inta

Tragoolpua

et al. 2019

Sci Rep

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Assam tea or Miang is a local name of Camellia sinensis var. assamica in northern Thailand. By the local wisdom, Assam tea leaves are used as the raw material in tea fermentation to produce “Fermented Miang” consumed by people in northern Thailand and the countries nearby. In this study, twenty-eight bacterial isolates were obtained from Assam tea leaf samples collected from Nan province, Thailand. Bacterial isolates were identified within 6 genera including Bacillus, Floricoccus, Kocuria, Lysinibacillus, Micrococcus and Staphylococcus. Among these, the strain ML061-4 shared 100.0 and 99.4% similarity of 16S rRNA and rpoB gene sequence with F. penangensis JCM 31735T, respectively. This is the first discovery of F. penangensis in Thailand. F. penangensis ML061-4 exhibited probiotic characteristics including lactic acid production (9.19 ± 0.10 mg/ml), antibacterial activities (Escherichia coli ATCC 25922 and E. coli O157:H7 DMST 12743), acid and bile salt tolerance (71.1 and 54.9%, respectively), autoaggregation (97.0%), coaggregation (66.0% with E. coli O157:H7), cell surface hydrophobicity (90.0%), bacterial adhesion (82.9% with Lactobacillus plantarum FM03-1), competitive inhibition (17.8% with E. coli O157:H7) and competitive exclusion (34.9% with E. coli O157:H7). Overall, the data suggested that F. penangensis ML061-4 had a great potential to be a probiotic.

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“…Bacterial isolates were characterized by the observation of colony morphology, Gram reaction, and string and motility tests. Cell morphology was investigated using SEM (JSM-IT300, JEOL Ltd., Tokyo, Japan) according to the protocol of Arroyo et al [ 29 ]. For biochemical identification, an evaluation of various tests was assessed, consisting of catalase, indole, lysine decarboxylase, lysine deaminase and cytochrome oxidase production, methyl red and Voges-Proskauer, nitrate reduction, citrate, triple sugar iron, gelatinase, urease and carbohydrate fermentation (including galactose, glucose, lactose, maltose, mannitol, mannose, rhamnose and sucrose) tests.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Bacterial Cellulose Produced by Komagataeibacter maltaceti P285 Isolated from Contaminated Honey Wine

et al. 2022

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Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family Acetobacteraceae. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with Komagataeibacter mal-taceti LMG 1529 T. K. maltaceti P285 produced the maximum BC production at 20–30 °C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, w/v) and yeast extract (3.2%, w/v) were applied as carbon and nitrogen sources, respectively. When sugarcane (8–16%, w/v) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by K. maltaceti P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2θ = 18.330, 21.390 and 22.640°). The maximum temperature of BC degradation was observed at 314 °C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that K. maltaceti P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories.

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Scanning electron microscopy of bacteria Tetrasphaera duodecadis

Cited by 15 publications

References 13 publications

Endophytes isolated from Panax notoginseng converted ginsenosides

Endophytes isolated from Panax notoginseng converted ginsenosides

Partial rpoB Gene Sequencing Identification and Probiotic Potential of Floricoccus penangensis ML061-4 Isolated from Assam Tea (Camellia sinensis var. assamica)

Characterization of Bacterial Cellulose Produced by Komagataeibacter maltaceti P285 Isolated from Contaminated Honey Wine

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