Scanning near-field optical/atomic force microscopy for fluorescence imaging and spectroscopy of biomaterials in air and liquid: Observation of recombinantEscherichia coli with gene coding to green fluorescent protein

Muramatsu, Hiroshi; Chiba, Norio; Ataka, Tatsuaki; Iwabuchi, Shinichiro; Nagatani, Naoki; Tamiya, Eiichi; Fujihira, Masamichi

doi:10.1007/bf02932050

Cited by 20 publications

(10 citation statements)

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“…45−49 However, the spatial resolution in a-SNOM is generally worse than in s-SNOM due to the reduction of collection efficiency with decreasing nanoscopic fiber aperture diameter. As a result, while ∼50 nm spatial resolution has been demonstrated at visible wavelengths, 47,49 only ∼1 μm spatial resolution has been achieved at infrared frequencies due to the longer wavelength and reduced fiber transmission. 48 Initial development of graphene-encapsulated liquid cells have allowed nanospectroscopic studies of aqueous samples using s-SNOM while circumventing the numerous issues of inliquid AFM operation.…”

Section: ■ Discussionmentioning

confidence: 99%

“…This work extends previous efforts of near-field imaging of biomolecules in liquid. Prior in-liquid applications of aperture-type scanning near-field optical microscopy ( a -SNOM) have minimized absorption by using tapered metallized optical fibers as local optical probes. − However, the spatial resolution in a -SNOM is generally worse than in s -SNOM due to the reduction of collection efficiency with decreasing nanoscopic fiber aperture diameter. As a result, while ∼50 nm spatial resolution has been demonstrated at visible wavelengths, , only ∼1 μm spatial resolution has been achieved at infrared frequencies due to the longer wavelength and reduced fiber transmission …”

Section: Discussionmentioning

confidence: 99%

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In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging

et al. 2020

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Imaging biological systems with simultaneous intrinsic chemical specificity and nanometer spatial resolution in their typical native liquid environment has remained a long-standing challenge. Here, we demonstrate a general approach of chemical nanoimaging in liquid based on infrared scattering scanning near-field optical microscopy (IR s-SNOM). It is enabled by combining AFM operation in a fluid cell with evanescent IR illumination via total internal reflection, which provides spatially confined excitation for minimized IR water absorption, reduced far-field background, and enhanced directional signal emission and sensitivity. We demonstrate in-liquid IR s-SNOM vibrational nanoimaging and conformational identification of catalase nanocrystals and spatio-spectral analysis of biomimetic peptoid sheets with monolayer sensitivity and chemical specificity at the few zeptomole level. This work establishes the principles of in-liquid and in situ IR s-SNOM spectroscopic chemical nanoimaging and its general applicability to biomolecular, cellular, catalytic, electrochemical, or other interfaces and nanosystems in liquids or solutions.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging

et al. 2020

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“…It is safely applicable for observation of soft samples with varying greatly in height. SNOAM may be also superior in liquid to other cyclic contact AFMs which use flat type cantilevers [181][182][183][184] because the optical-fiber cantilever of SNOAM is round, which helps to reduce the viscous resistance of the liquid. SNOAM is an excellent tool to observe biological materials because it simultaneously provides topographic and optical images with high resolution.…”

Section: Principle Of the Nano-diagnosis Based On The Snoammentioning

confidence: 98%

The principles and applications of nano-diagnosis system for a nano-biosensor

Kim

Chang

Muramatsu

et al. 2011

Korean J. Chem. Eng.

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In this review, the basic principles and research trends of biosensors are briefly described and a nano-sensing system applying QCM (quartz crystal micro-balance), nano-diagnosis methods by AFM (atomic force microscopy) and SNOAM (scanning near-field/atomic force microscopy) is discussed intensively. The principle, construction, and applications of piezoelectric crystal sensors as a universal sensor are reviewed. This review is focused mainly on liquid phase applications, such as immune-sensors, gelation detecting sensors, and cultured cell monitoring sensors. The principle of nano-diagnosis based on the AFM or SNOAM techniques is described in detail. Finally, the binding affinity of peptide probes to proteins using AFM and the visualizing of a hybridized PNA probe on a DNA molecule using SNOAM are evaluated and discussed.

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“…The feedback mechanism is based on the strong distance-dependence of evanescent waves, which decay exponentially above the surface. 207 There are a multitude of NSOM operation modes, including transmission/ illumination 208 and apertureless mode, 209 and also many styles of probes (e.g. an etched, metallic coated optical ber 210 ).…”

Section: Near-eld Scanning Optical Microscopy-atomic Force Microscop...mentioning

confidence: 99%

Atomic force microscopy-based bioanalysis for the study of disease

Morton

Baker

2014

Anal. Methods

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Scanning near-field optical/atomic force microscopy for fluorescence imaging and spectroscopy of biomaterials in air and liquid: Observation of recombinantEscherichia coli with gene coding to green fluorescent protein

Cited by 20 publications

References 14 publications

In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging

In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging

The principles and applications of nano-diagnosis system for a nano-biosensor

Atomic force microscopy-based bioanalysis for the study of disease

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