Scar Tissue Distribution on Palates and its Relation to Maxillary Dental Arch Form

Ishikawa, Hiroyuki; Nakamura, Shinji; Misaki, Kouichi; Kudoh, Motonori; Fukuda, Hiroshi; Yoshida, Shigemitsu

doi:10.1597/1545-1569_1998_035_0313_stdopa_2.3.co_2

Cited by 39 publications

(21 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased amount of collagen type I fibers indicates an increased tensile strength of the submucosa. The increase in tensile strength of the submucosa is one of the factors that impairs maxillary growth after cleft palate surgery (1,3,4).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Myofibroblasts and matrix components in healing palatal wounds in the rat

Cornelissen¹,

Stoop

Hoff³

et al. 2000

J Oral Pathology Medicine

View full text Add to dashboard Cite

In order to identify wound contraction and scar formation during palatal mucoperiosteal wound healing in growing rats, the temporal and spatial distribution of myofibroblasts and matrix components were determined immunohistochemically. Myofibroblasts were found in the mucosal part of the palatal wound tissue between 4 and 22 days, with the highest density at 8 days post-wounding. The number of collagen type I and type III fibers gradually increased until about 8 days postwounding, and thereafter the staining intensity of collagen type III decreased. At 60 days post-wounding there were more transversely oriented collagen type I fibers and less type III fibers and elastin present in the submucosa than in normal tissue. The results suggest that in this model wound contraction mainly takes place in the mucosa between 4 and 22 days postwounding. Furthermore, palatal wounds made in young rats heal with distinct scar tissue formation. Therefore, this model is useful to test the effects of therapies that aim to reduce wound contraction and scarring after cleft palate surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Cleft palate surgery in young patients is considered to be one of the factors causing disturbances in maxillary growth and dento-alveolar development (1). The adverse effects of surgery have been attributed to wound contraction and scarring in the remaining palatal wound (2)(3)(4).…”

mentioning

confidence: 99%

Myofibroblasts and matrix components in healing palatal wounds in the rat

Cornelissen¹,

Stoop

Hoff³

et al. 2000

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…1 However, this surgery always causes the denuded bone surface on the palate, thereafter, the scar tissue formation in the open wounds is the major reason accounted for the maxillary growth retardation. 2 Therefore, primary closure of the wounds would be the reasonable intervention for these iatrogenic effects. 3 However, there is still no autologous tissue grafts used for the coverage of the wounds in palatoplasty.…”

Section: Introductionmentioning

confidence: 99%

In vitro engineering of a palatal mucosa equivalent with acellular porcine dermal matrix

Xiong

Zhao

Zhang

et al. 2007

J Biomedical Materials Res

View full text Add to dashboard Cite

The objective of this study was to develop a palatal mucosa equivalent composed of multilayered oral keratinocytes grown on the acellular porcine dermal matrix. Acellular porcine dermal matrix was prepared through a series of procedures and assessed by histological, immunohistochemical, and scanning electron microscopy examination. The palatal mucosa equivalent was fabricated by seeding oral keratinocytes, which cultured from human palate mucosa, onto the acellular dermal matrix. After 4 days submerged in medium, this composite was raised to the air-liquid interface for another 7 or 14 days of cultivation. The results demonstrated the processed porcine dermal matrix was totally cell-free. The resultant palatal mucosa equivalent showed a multilayered oral epithelium that had been formed, and the number of cell layers was correlated with the culture period at the air-liquid interface. Oral keratinocytes infiltrated into the empty hair follicles of the acellular porcine dermal matrix and formed an anchor-like structure, which exhibited resemblance to the rete ridges of the native palate mucosa. Immunohistochemical staining for CK10/13, CK19, Ki-67 nuclear antigen, and Heparan sulphate indicated the cultured palatal mucosa equivalent shared the same characteristics with that of the native palate mucosa. In conclusion, our fabricated palatal mucosa equivalent exhibited the characteristics of the native counterpart, and this equivalent might be useful for recovery of the wounds in the palate secondary to palatoplasty.

show abstract

“…It has been strongly suggested that this scar tissue is a major factor in the impairment of skeletal growth and development of the dentition in both cleft palate patients and animal models. [1][2][3] Myofibroblasts are probably involved in wound contraction. 4,5 These contractile fibroblasts are numerous in many types of contracting wounds.…”

mentioning

confidence: 99%

Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

Ophof

Maltha

Hoff

et al. 2004

Wound Repair Regeneration

View full text Add to dashboard Cite

Tissue shortage complicates the surgery of cleft lip and palate anomalies and the healing of defects on the palate impairs growth of the dento-alveolar complex due to scar tissue formation. Implantation of substitutes into the wound area might overcome this adverse effect. The aim of this study was to compare the tissue response to three collagen-based (collagen type I substrate alone, or collagen coated with elastin or chondroitin-6-sulfate) and two skin-derived substrates (unprocessed dermis and AlloDerm) after implantation into 12 dogs. Histology was performed at 3, 10, and 20 days postsurgery. We showed that all substrates were well tolerated. However, it is unclear whether AlloDerm was rapidly degraded or if it was sequestrated. There was no elastin or collagen present in these wounds. All collagen-based substrates showed good epithelial regeneration, although heparan sulfate (JM 403) was absent. Wounds treated with the collagen-based substrates contained fewer myofibroblasts at 20 days postsurgery and the type III collagen fibers in the immature scar tissue were more randomly oriented than in an untreated wound. In conclusion, palatal wounds with a dermal substrate heal with fewer indications of scar tissue formation and evoke only a mild inflammatory reaction, which is preferred over the tissue reaction in an untreated wound.

show abstract

Scar Tissue Distribution on Palates and its Relation to Maxillary Dental Arch Form

Cited by 39 publications

References 8 publications

Myofibroblasts and matrix components in healing palatal wounds in the rat

Myofibroblasts and matrix components in healing palatal wounds in the rat

In vitro engineering of a palatal mucosa equivalent with acellular porcine dermal matrix

Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

Contact Info

Product

Resources

About