Schisandrin C improves leaky gut conditions in intestinal cell monolayer, organoid, and nematode models by increasing tight junction protein expression

Kim, Mi Ri; Cho, Suyeon; Lee, Hee Ju; Kim, Joo Yeon; Nguyen, Uyen Tran Tu; Ha, Ngoc Minh; Choi, Ki Young; Hyun, Kwang; Kim, Jeong-Ho; Kim, Won Kyu; Kang, Kyungsu

doi:10.1016/j.phymed.2022.154209

Cited by 20 publications

(9 citation statements)

References 53 publications

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“…However, our results cannot rule out that intestinal leakiness contributes to this result. Again, such a phenotype may involve changes in collagen composition of the basement membrane, but may also be a consequence of defective intestinal cell-cell junctions (29,30)).…”

Section: Discussionmentioning

confidence: 99%

A VHL-1;HIF-1/SQRD1/COL-88 axis links extracellular matrix formation with longevity inCaenorhabditis elegans

Salgueiro,

Esmaillie,

Bohl

et al. 2024

Preprint

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The extracellular matrix (ECM) is a pivotal three-dimensional network crucial for tissue organization, cellular communication, and fundamental cellular processes, where collagens are the major chemical entity in amount. ECM deregulation is directly involved with several pathologies, such as tumour growth and invasiveness, atherosclerosis, and diabetic nephropathy. Mutations in the von Hippel-Lindau tumour suppressor (pVHL) cause VHL syndrome, a multi-tumour syndrome commonly associated with clear cell renal carcinoma (ccRCC). Loss of pVHL is associated with the activation of hypoxia-inducible factor (HIF) signaling. Mutation of VHL-1 in the nematode Canorhabditis elegans has been shown to increase lifespan and stress resistance. Interestingly, considering recent findings on the involvement of collagens in the regulation of lifespan, we also observed these animals to show defects in body morphology in a HIF-1 dependent manner. Based on this finding, we established a link between HIF-1 activation upon loss of VHL-1 and ECM defects associated with alterations in collagen expression. An RNAi screen examining genes upregulated in vhl-1 mutant worms revealed the sulfide quinone oxidoreductase sqrd-1 to mediate the change in body morphology. SQRD-1 is essential to the HIF-1 dependent increase in several collagen genes. One of these genes, col-88, partly mediates both the impact of loss of VHL-1 on lifespan extension and body length. The downregulation of the uncharacterised col-88 partially restores lifespan extension and reduces body size of vhl-1/sqrd-1 to vhl-1(ok161) single mutant. This study contributes to the increasing body of evidence linking lifespan extension and the ECM and now implicates this axis in hypoxia-signaling. These findings are of special interest considering the role of ECM integrity in tumour growth and metastasis.

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Section: Discussionmentioning

confidence: 99%

A VHL-1;HIF-1/SQRD1/COL-88 axis links extracellular matrix formation with longevity inCaenorhabditis elegans

Salgueiro,

Esmaillie,

Bohl

et al. 2024

Preprint

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“…This result was similar to the research results on the 2D-cell culture with the human intestinal Caco-2 cells model, which is also shown in this study. It demonstrates the compatibility between 2D-cell culture and 3D-cell culture models in studying the molecular mechanism of biologically active compounds [55]. Another study by Cai et al was also performed using intestinal organoids to determine the effect of some common dietary nutrients, such as caffeic acid, chlorogenic acid, L-glutamic acid, curcumin, vitamin C, and m-hydroxyphenylpropionic acid on the growth of this organoid.…”

Section: D-cell Culturementioning

confidence: 96%

Bioactivity and toxicity evaluation of nutraceuticals using in vitro cell-based models: A review

Hung

2022

VJFC

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Nutraceutical is one of the new concepts appearing in recent years, referring to food products derived from natural sources that benefit human health in preventing and treating diseases, besides providing nutritional value. During the development of these products, it is important to evaluate the toxicity and bioactivity of potential compounds and to study the mechanism of action of these nutraceutical compounds at the cellular and molecular levels. Among the many different experimental models, the in vitro cell-based model has emerged as a model with many advantages for preliminary screening of nutraceuticals activity and toxicity before conducting further studies on in vivo models. This review summarizes some basic techniques commonly used to screen nutraceuticals' toxicity. Recent studies of the bioactivity of nutraceuticals in various areas, such as antioxidant, anti-inflammation, antiobesity, neuroprotection, and gut health improvement, are also reviewed to introduce the application of the cell-based model in nutraceutical bioactivity research. New modern techniques using in vitro cell-based models have been applied in this field, such as highthroughput screening, 3D-cell culture, and organ-on-a-chip are also discussed in this paper.

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“…Consequently, this process facilitates the movement of LPSs from the intestines into the bloodstream [ 13 ]. These occurrences result in a disparity between the host and microbial communities, compromise barrier function, and are characterized by abnormally high intestinal permeability and altered epithelial TJ molecule expression [ 14 ].…”

Section: Hfdmentioning

confidence: 99%

“…Several research studies have demonstrated that HFDs elevate the levels of endotoxin in the body's circulation, leading to a decrease in the expression of tight junction (TJ) proteins such as zonula occludens (ZO)-1, occludin, and claudin as they interact with the barrier [ 25 , 26 ]. Furthermore, hazardous chemicals can enter the intestine due to malfunctions of the intestinal barrier, impairing the immune system and promoting inflammation in the intestine, which can also lead to inflammatory bowel disease (IBD) [ 14 ]. Several studies have highlighted that an HFD has a negative impact on human health based on its association with obesity, type 2 diabetes, hypertension, IBD, leaky gut, and cardiovascular diseases [ 25 , 27 - 29 ].…”

Section: Hfdmentioning

confidence: 99%

Diet-Induced Gut Dysbiosis and Leaky Gut Syndrome

Chae,

Lee,

Kim

et al. 2024

J. Microbiol. Biotechnol.

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Chronic gut inflammation promotes the development of metabolic diseases such as obesity. There is growing evidence which suggests that dysbiosis in gut microbiota and metabolites disrupt the integrity of the intestinal barrier and significantly impact the level of inflammation in various tissues, including the liver and adipose tissues. Moreover, dietary sources are connected to the development of leaky gut syndrome through their interaction with the gut microbiota. This review examines the effects of these factors on intestinal microorganisms and the communication pathways between the gut-liver and gut-brain axis. The consumption of diets rich in fats and carbohydrates has been found to weaken the adherence of tight junction proteins in the gastrointestinal tract. Consequently, this allows endotoxins, such as lipopolysaccharides produced by detrimental bacteria, to permeate through portal veins, leading to metabolic endotoxemia and alterations in the gut microbiome composition with reduced production of metabolites, such as short-chain fatty acids. However, the precise correlation between gut microbiota and alternative sweeteners remains uncertain, necessitating further investigation. This study highlights the significance of exploring the impact of diet on gut microbiota and the underlying mechanisms in the gut-liver and gut-brain axis. Nevertheless, limited research on the gut-liver axis poses challenges in comprehending the intricate connections between diet and the gut-brain axis. This underscores the need for comprehensive studies to elucidate the intricate gut-brain mechanisms underlying intestinal health and microbiota.

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Schisandrin C improves leaky gut conditions in intestinal cell monolayer, organoid, and nematode models by increasing tight junction protein expression

Cited by 20 publications

References 53 publications

A VHL-1;HIF-1/SQRD1/COL-88 axis links extracellular matrix formation with longevity inCaenorhabditis elegans

A VHL-1;HIF-1/SQRD1/COL-88 axis links extracellular matrix formation with longevity inCaenorhabditis elegans

Bioactivity and toxicity evaluation of nutraceuticals using in vitro cell-based models: A review

Diet-Induced Gut Dysbiosis and Leaky Gut Syndrome

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