Schistosomiasis mansoni in Bananal (State of São Paulo, Brazil): III. Seroepidemiological studies in the Palha District

Zacharias, Fabiana; Carvalho, Maria Esther de; Gargioni, Cybele; Teles, Horacio Manuel Santana; Ferreira, Cláudio Santos; Lima, Valquíria R. de

doi:10.1590/s0074-02762002000900005

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…In Bananal, from the year 2001 onwards, we started to examine two slides from each fecal sample, in addition to higher volumes of feces per slide, using, as routine techniques, microscopy of pressed fecal samples and serological examinations 21,23 . Such routine was responsible for the maintenance of the efficiency of the active surveillance of new cases during the interval from 2001 to 2003.…”

Section: Discussionmentioning

confidence: 99%

Assessment of control and epidemiologic details of the schistosomiasis mansoni in Bananal, São Paulo, Brazil

Teles

Ferreira

Carvalho

2014

Rev. bras. epidemiol.

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AbstrAct:The purpose of our study in the municipality of Bananal, state of São Paulo, Brazil, was to stop the transmission of schistosomiasis mansoni. Particular emphasis was given to such items as the active surveillance and eventual treatment of hosts, mapping parasite foci, and increasing the extent of basic sanitation in the municipality. Now, our records indicate that the eradication of schistosomiasis in the municipality of Bananal is attainable. However, as the vector Biomphalaria tenagophila can still be found in some water bodies within this municipality, it remains included in the area where schistosomiasis is endemic, which calls for very strict measures to avoid the human cases of schistososomiasis. The expansion of the coverage of the basic sanitation network and treatment of Schistosoma mansoni cases diagnosed during periodic surveys are part of the plans to eradicate schistosomiasis in Bananal.

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Section: Discussionmentioning

confidence: 99%

Assessment of control and epidemiologic details of the schistosomiasis mansoni in Bananal, São Paulo, Brazil

Teles

Ferreira

Carvalho

2014

Rev. bras. epidemiol.

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Evaluation of the sensitivity of IgG and IgM ELISA in detecting Schistosoma mansoni infections in a low endemicity setting

Espírito-Santo

Sanchez

et al. 2014

Eur J Clin Microbiol Infect Dis

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Schistosomiasis is a major public health concern, with 200 million people infected worldwide. In Brazil, this disease has been reported in 19 states, and its prevalence in the city of Barra Mansa in Rio de Janeiro State is 1 %. The parasitological diagnostic methods currently available in these areas lack sensitivity; however, enzyme-linked immunosorbent assays (ELISAs) have been employed successfully for the diagnosis of schistosomiasis by using antibodies against antigens of Schistosoma mansoni adult worms and eggs, and for the detection of circulating antigens. The objective of this study was to determine systematically the prevalence of S. mansoni infection in the peripheral areas of Barra Mansa. A cross-sectional study was conducted from April to December 2011 by using probabilistic sampling that collected 610 fecal samples and 612 serum samples. ELISA-IgG with total extracts and ELISA-IgM with trichloroacetic acid-soluble fractions were employed to detect antibodies against S. mansoni and were compared with the Kato-Katz and Hoffman parasitological techniques. Among the individuals studied, anti-S. mansoni antibodies were detected in 11.16 % (n = 71) by ELISA-IgG and in 20.75 % (n = 132) by ELISA-IgM, while the parasitological techniques showed 0.82 % (n = 5) positivity. The agreement between the two ELISA tests was 85.38 % (n = 543), and 8.65 % (n = 55) of the serum samples showed positive results in both tests. The higher positivity of the ELISA-IgM test corroborates the results of previous reports and indicates that the test may be a useful tool in epidemiological studies, particularly in areas of low endemicity for S. mansoni.

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Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

Espírito-Santo

Alvarado-Mora

Pintó

et al. 2015

BioMed Research International

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Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity (P < 0.05), and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.

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Schistosomiasis mansoni in Bananal (State of São Paulo, Brazil): III. Seroepidemiological studies in the Palha District

Cited by 6 publications

References 11 publications

Assessment of control and epidemiologic details of the schistosomiasis mansoni in Bananal, São Paulo, Brazil

Assessment of control and epidemiologic details of the schistosomiasis mansoni in Bananal, São Paulo, Brazil

Evaluation of the sensitivity of IgG and IgM ELISA in detecting Schistosoma mansoni infections in a low endemicity setting

Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

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