Scientific Opinion on the safety and efficacy of vitamin B2 (80 %) as riboflavin produced by Bacillus subtilis for all animal species, based on a dossier submitted by VITAC EEIG

,

doi:10.2903/j.efsa.2014.3531

Cited by 13 publications

(29 citation statements)

References 10 publications

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“…This conclusion also applies to riboflavin 5 0 -phosphate. In its previous opinions on vitamin B 2 , the FEEDAP Panel supported the conclusions of the ANS Panel concerning the vitamin 'per se' (EFSA FEEDAP Panel, 2014, 2018b.…”

Section: Toxicological Studiesmentioning

confidence: 88%

“…The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) issued two opinions on the safety and efficacy of vitamin B 2 (80%) as riboflavin produced by Bacillus subtilis KCCM-10445 for all animal species (EFSA FEEDAP Panel, 2014, 2018a, an opinion on the safety and efficacy of vitamin B 2 as riboflavin and riboflavin-5 0 -phosphate ester monosodium salt, produced by either Bacillus subtilis DSM 17339 or Bacillus subtilis DSM 23984 (EFSA FEEDAP Panel, 2016), and another opinion on the safety and efficacy of vitamin B 2 (riboflavin) produced by Ashbya gossypii (EFSA FEEDAP Panel, 2018b).…”

Section: Additional Informationmentioning

confidence: 99%

“…The nutrient requirements and recommendations for the target species, their tolerance limits to riboflavin excess and the toxic effect of riboflavin depending on the administration route were discussed in previous opinions (EFSA FEEDAP Panel, 2014. According to the National Research Council (NRC), requirements for vitamin B 2 are in the range of 1.7-4.0 mg/kg feed for poultry (NRC, 1994), 2-4 mg/kg for pigs (NRC, 2012), 2.7-25 mg/kg for fish (NRC, 2011) and 2.0-4.2 mg/kg for companion animals (NRC, 2006(NRC, , 2007a.…”

Section: Safety For the Target Speciesmentioning

confidence: 99%

“…Riboflavin 5 0 -phosphate sodium is rapidly dephosphorylated to riboflavin in the intestinal mucosa (Christensen, 1969). 34 Riboflavin absorption, its metabolic fate and its potential accumulation in edible tissues and eggs have been described in detail in previous opinions (EFSA FEEDAP Panel, 2014.…”

Section: Metabolic and Residue Studiesmentioning

confidence: 99%

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Safety and efficacy of vitamin B2 (riboflavin 5′‐phosphate ester monosodium salt) for all animal species when used in water for drinking

Additives¹,

Bampidis²,

Azimonti³

et al. 2018

EFS2

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Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on vitamin B 2 in the form of riboflavin 5 0 -phosphate ester monosodium salt as an additive for all animal species for use in water for drinking. The additive under assessment is obtained from a source of riboflavin produced by Ashbya gossypii. No information was provided on the identity and characterisation of the production strain, and on whether or not it is a genetically modified microorganism. Therefore, in the absence of adequate information, it is not possible to perform an assessment of the safety of the production strain. Riboflavin 5 0 -phosphate ester monosodium salt 'per se' is considered safe for the target animals, consumers and the environment. In the absence of data regarding the identity and characterisation of the production strain, the FEEDAP Panel cannot conclude on the safety for the target species, consumers, users and the environment of the riboflavin 5 0 -phosphate ester monosodium salt under assessment. Similarly, in the absence of data, no conclusion can be reached on the safety of the product for the users. Riboflavin 5 0 -phosphate ester monosodium salt is regarded as effective in covering the animal's requirement when administered via water for drinking. and Tarres-Call J, 2018. Safety and efficacy of vitamin B 2 (riboflavin 5 0 -phosphate ester monosodium salt) for all animal species when used in water for drinking. EFSA Journal 2018;16(12):5531, 15 pp. https://doi.org/10.

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Section: Toxicological Studiesmentioning

confidence: 88%

Section: Additional Informationmentioning

confidence: 99%

Section: Safety For the Target Speciesmentioning

confidence: 99%

Section: Metabolic and Residue Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Safety and efficacy of vitamin B2 (riboflavin 5′‐phosphate ester monosodium salt) for all animal species when used in water for drinking

Additives¹,

Bampidis²,

Azimonti³

et al. 2018

EFS2

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“…Substances used in Animal Feed (FEEDAP) issued an opinion on the safety and efficacy of vitamin B 2 (80%) as riboflavin produced by B. subtilis for all animal species(EFSA FEEDAP Panel, 2014a).…”

mentioning

confidence: 99%

Safety and efficacy of vitamin B2 (riboflavin and riboflavin 5’‐phosphate ester monosodium salt) produced by Bacillus subtilis for all animal species based on a dossier submitted by DSM

Aquilina¹,

Bampidis²,

Bastos³

et al. 2016

EFS2

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2Riboflavin is produced by two genetically modified Bacillus subtilis strains. Neither the production strains nor their recombinant DNA were detected in the final riboflavin products. Riboflavin 5'phosphate sodium is prepared by phosphorylation of riboflavin. The additives are safe for target animals with a wide margin of safety provided that the current use levels for riboflavin are not exceeded. Toxicological studies with the additive under assessment show that it has a low toxicity and do not indicate a genotoxic potential. The use of the additives in animal nutrition will not significantly alter the riboflavin content of food of animal origin. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) considers that the use of the additives in animal nutrition is not of safety concern for consumers. The additives containing riboflavin and riboflavin 5'-phosphate sodium are not irritant to skin and eyes. In the absence of data, the FEEDAP Panel cannot conclude on skin sensitisation. Riboflavin is a recognised photosensitiser, which may elicit skin and eye photoallergic reactions. Workers might be exposed to a respirable dust when handling riboflavin and riboflavin 5'-phosphate sodium; in the absence of data on inhalation toxicity, the FEEDAP Panel cannot conclude on a possible risk by inhalation. The use of riboflavin and riboflavin 5'-phosphate sodium in animal nutrition does not pose a risk to the environment. The additives are regarded as an effective source of riboflavin in covering the animal's requirement when administered orally.

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Production of vitamin B2 (riboflavin) by Bacillus subtilis

Chu

Wang

et al. 2022

J of Chemical Tech & Biotech

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Riboflavin is one of the main micronutrients required for the human body for cellular respiration. It is the precursor of flavin mononucleotide and flavin adenine dinucleotide coenzymes crucial for the production of energy. The development of biotechnology has given rise to microbial procedures that strongly compete with the chemical synthesis approach and hence the fermentation process of riboflavin by microorganisms was initiated. Therefore, the main aim of this review is to investigate the production of riboflavin by Bacillus subtilis fermentation. The introduction of the microbial synthesis method has found that regulatory and structural genes involved in the synthesis of vitamin B2 are overexpressed, which has eventually enhanced the strain productivity and production of the vitamin via industrial fermentation. Using B. subtilis to synthesize riboflavin can reduce the production cost and increase the yield of riboflavin. The emergence of microbial synthesis methods has effectively increased the yield of riboflavin, and the study of the process of riboflavin synthesis by B. subtilis is of great significance for improving production, reducing costs and using renewable raw materials. © 2021 Society of Chemical Industry (SCI).

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Scientific Opinion on the safety and efficacy of vitamin B2 (80 %) as riboflavin produced by Bacillus subtilis for all animal species, based on a dossier submitted by VITAC EEIG

Cited by 13 publications

References 10 publications

Safety and efficacy of vitamin B2 (riboflavin 5′‐phosphate ester monosodium salt) for all animal species when used in water for drinking

Safety and efficacy of vitamin B2 (riboflavin 5′‐phosphate ester monosodium salt) for all animal species when used in water for drinking

Safety and efficacy of vitamin B2 (riboflavin and riboflavin 5’‐phosphate ester monosodium salt) produced by Bacillus subtilis for all animal species based on a dossier submitted by DSM

Production of vitamin B2 (riboflavin) by Bacillus subtilis

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