<scp>GPR12</scp> inhibits migration and promotes apoptosis in esophageal cancer and hypopharyngeal cancer cells

Zhang, Minfa; Yang, Xiaoqi; Chen, Shuai; Jia, Wenming; Ma, Xiaojie; Wang, Juan; Qian, Yue; Lei, Dapeng; Liu, Heng; Pan, Xinliang

doi:10.1111/1759-7714.13933

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…4 A). Based on the literatures included in PubMed, GPR12, ITM2B, ZNF24, and NSL1 were selected as the candidate targets due to the low expressions in various cancers [ 17 – 20 ]. We examined the mRNA expressions of these four genes in T24 cells, and GPR12 showed the most significant downregulation after miR-105-5p mimic transfection (Fig.…”

Section: Resultsmentioning

confidence: 99%

Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancer

Pan,

Jiang,

et al. 2023

BMC Urol

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Bladder cancer stem cells (BCSCs) are considered as the root cause of BC initiation and recurrence, and exosomes derived from BCSCs (CSCs-exo) are the vital tool for establishing a stable tumor microenvironment. miR-105-5p has been revealed to promote tumor growth in a variety of cancers, but the effects on BC are still not included.Characteristics of CSCs-exo were examined by transmission electron microscope and nanoparticle tracking analysis. PKH67 dye was used to observe the cellular uptake of exosomes. Cell viability, migration and invasion were detected by CCK-8, wound healing and transwell invasion assays, respectively. The interaction between miR-105-5p and GPR12 was verified by luciferase activity assay. Xenografts were induced in the nude mice, and H&E staining method was applied to analyze the histological changes of xenografts. CSCs-exo efficiently promoted BC cell viability, migration and invasion. miR-105-5p was highly expressed in CSCs and CSCs-exo treatment significantly upregulated the expression of miR-105-5p in BC cells.GPR12 was subsequently verified to be the target gene of miR-105-5p, and overexpression of GPR12 abrogated the effects of miR-105-5p on BC cell growth and metastasis. Reversely, the anti-tumor function of miR-105-5p antagomir was observed in the xenograft mice.CSCs aggravated the malignancy of BC partly through transmitting exosomal miR-105-5p to BC cells to inhibit the expression of GPR12, which developed a novel aspect for CSC-targeted therapies.

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Section: Resultsmentioning

confidence: 99%

Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancer

Pan,

Jiang,

et al. 2023

BMC Urol

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“…A recent study showed that GPR12 induces apoptosis by activating caspase-7, and inhibits the migration of hypopharyngeal, laryngeal, and esophageal cancer cells by promoting the expression of EMT-related proteins in hypopharyngeal, laryngeal, and esophageal cancer cells (46). The work of Hala El-Zimaity et al showed that Helicobacter pylori is associated with esophageal cancer, leading to increased gastric acid secretion.…”

Section: Discussionmentioning

confidence: 99%

Is Laryngeal Squamous Cell Carcinoma Related to Helicobacter pylori?

Huang

et al. 2022

Front. Oncol.

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BackgroundLaryngeal carcinoma is a primary malignant tumor originating from the laryngeal mucosa. In recent years, an increasing number of studies have confirmed that Helicobacter pylori may play a role in the occurrence and development of laryngeal cancer. We conducted a systematic review and meta-analysis to identify and emphasize the relationship between laryngeal cancer and Helicobacter pylori infection.MethodsWe actively searched for systematic reviews of PubMed, Embase, Web of Science, and Cochrane libraries to select studies that met the recommended guidelines. A total of 1756 patients with laryngeal cancer were included in this study to assess the association of Helicobacter pylori in the larynx with laryngeal cancer. A subassessment of the risk of bias for each study that met the inclusion criteria was carried out. To illustrate the reasons for heterogeneity, we performed a subgroup analysis to determine the type of study, the quality of the article, the diagnostic method, and the impact of exposure factors.ResultsThe meta-analysis included a total of 17 case-control studies on the association between Helicobacter pylori in the larynx and laryngeal cancer. Our meta-analysis showed that Helicobacter pylori infection in the larynx significantly increased the risk of malignant tumors in the larynx (odds ratio, 2.96; 95% CI, 1.83-4.78; P<0.00001; I2 = 86%). They still existed when we controlled for patients’ exposure to smoking factors (odds ratio, 3.86; 95% CI, 1.89–7.88).ConclusionThis systematic review and meta-analysis showed an association between Helicobacter pylori and laryngeal cancer. These findings are consistent with the understanding that chronic inflammatory tissue insult may lead to the development of malignancy. Controlling risk factors will help us identify patients with an increased risk of laryngeal cancer.

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“…In pancreatic cancer cells (PANC-1) cells, Park et al observed that overexpression of GPR12 induced K8 phosphorylation and perinuclear reorganization, promoting migration and invasion by changing the viscoelasticity (13). However, Zhang et al demonstrated that GPR12 as a potential tumor suppressor-mediated cell migration and apoptosis in esophageal cancer and hypopharyngeal cancer (21), indicating that the characteristics of GPR12 varied in different types of cancer. Here, we found that GPR12 mRNA expression is extremely low in the majority of cancer types and significantly decreased in glioblastoma multiforme (GBM), brain lower grade glioma (LGG), and skin cutaneous melanoma (SKCM) tissues than matched normal tissues through pan-cancer analysis of GEPIA (Supplementary Figure 2A).…”

Section: Discussionmentioning

confidence: 99%

GPR12 Inhibits Apoptosis in Epithelial Ovarian Cancer via the Activation of ERK1/2 Signaling

Wang

Zhang

et al. 2022

Front. Oncol.

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Epithelial ovarian cancer (EOC) is one of the most lethal gynecological malignancies in women worldwide. G protein–coupled receptor 12 (GPR12) is a member of G protein–coupled receptors (GPCRs) and plays an important role in the regulation of cell proliferation and survival. However, its role in EOC is underappreciated. In this study, we found that GPR12 is highly expressed in the EOC tissues and can be an ideal biomarker to predict the prognosis of patients with EOC. GPR12 knockdown obviously inhibits the proliferation of EOC cells by inducing cellular apoptosis in vitro and in vivo. Meanwhile, bioinformatic analysis showed that the inhibitory effect of GPR12 knockdown on the cell viability is closely related with Extracellular signal-regulated kinases 1/2 (ERK1/2) pathway, which has been confirmed by the fact that the activity of ERK1/2 pathway has been significantly blocked in the GPR12 knockdown cells. LM22B-10, ERK1/2 pathway activator, could reverse the inhibited proliferation caused by GPR12 knockdown in the EOC cells. Our findings suggest that GPR12 is involved in the EOC process and is a potential therapeutic target for EOC.

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GPR12 inhibits migration and promotes apoptosis in esophageal cancer and hypopharyngeal cancer cells

Cited by 8 publications

References 30 publications

Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancer

Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancer

Is Laryngeal Squamous Cell Carcinoma Related to Helicobacter pylori?

GPR12 Inhibits Apoptosis in Epithelial Ovarian Cancer via the Activation of ERK1/2 Signaling

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