<scp>IL</scp>‐6 regulated stress‐induced <scp>R</scp>ex‐1 expression in stem cells from human exfoliated deciduous teeth

Govitvattana, Nattanan; Osathanon, Thanaphum; Taebunpakul, Suthisa; Pavasant, Prasit

doi:10.1111/odi.12052

Cited by 19 publications

(38 citation statements)

References 60 publications

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“…Thus, our results confirm the stem cell properties of cells isolated from the dental pulp tissue of human exfoliated deciduous teeth. These findings are consistent with those of previous studies [Govitvattana et al, 2013;Osathanon et al, 2013a;Nowwarote et al, 2015a].…”

Section: Discussionsupporting

confidence: 94%

“…Cycloheximide pretreatment resulted in the attenuation of the bFGF‐induced REX1 expression, indicating the involvement of intermediate molecule(s). A previous study reported that IL‐6 treatment increased REX1 expression in SHEDs [Govitvattana et al, ]. Interestingly, the results in the present study demonstrated that bFGF regulated IL‐6 expression in SHEDs via the FGFR and Akt signaling pathways similar to that of the bFGF regulated REX1 expression.…”

Section: Discussionsupporting

confidence: 74%

“…IL‐6 attenuated the chondrogenic differentiation of bone marrow derived mesenchymal stem cells (BM‐MSCs) [Wei et al, ]. In SHEDs, Previous reports demonstrated that IL‐6 induced REX‐1 expression via the JAK and ATP‐P2Y1 signaling pathways [Govitvattana et al, , ]. In the present study, we found that bFGF induced REX‐1 expression partly via IL‐6, suggesting that IL‐6 plays a role in stemness maintenance in SHEDs.…”

Section: Discussionsupporting

confidence: 65%

“…via the JAK and ATP-P2Y1 signaling pathways [Govitvattana et al, 2013[Govitvattana et al, , 2015. In the present study, we found that bFGF induced REX-1 expression partly via IL-6, suggesting that IL-6 plays a role in stemness maintenance in SHEDs.…”

Section: Discussionsupporting

confidence: 56%

“…IL-6 is generally known as a cytokine that regulates inflammatory responses. However, other studies indicate that IL-6 is also an important molecule in stemness maintenance and cell differentiation [Pricola et al, 2009;Gallagher et al, 2013;Govitvattana et al, 2013Govitvattana et al, , 2015Yoon et al, 2014]. It has been shown that IL-6 supplementation together with soluble IL-6 receptor enhanced the osteogenic differentiation of periodontal ligament cells and adipose derived stem cells [Iwasaki et al, 2008;Huh and Lee, 2013].…”

Section: Discussionmentioning

confidence: 99%

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Basic Fibroblast Growth Factor Regulates REX1 Expression Via IL‐6 In Stem Cells Isolated From Human Exfoliated Deciduous Teeth

Nowwarote

Sukarawan²,

Pavasant

et al. 2016

J of Cellular Biochemistry

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Basic fibroblast growth factor (bFGF) regulates pluripotent marker expression and cellular differentiation in various cell types. However, the mechanism by which bFGF regulates REX1 expression in stem cells, isolated from human exfoliated deciduous teeth (SHEDs) remains unclear. The aim of the present study was to investigate the regulation of REX1 expression by bFGF in SHEDs. SHEDs were isolated and characterized. Their mRNA and protein expression levels were determined using real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. In some experiments, chemical inhibitors were added to the culture medium to impede specific signaling pathways. Cells isolated from human exfoliated deciduous tooth dental pulp tissue expressed mesenchymal stem cell surface markers (CD44, CD73, CD90, and CD105). These cells differentiated into osteogenic and adipogenic lineages, when appropriately induced. Treating SHEDs with bFGF induced REX1 mRNA expression and this effect was attenuated by pretreatment with FGFR or Akt inhibitors. Cycloheximide pretreatment also inhibited the bFGF-induced REX1 expression, implying the involvement of intermediate molecule(s). Further, the addition of an IL-6 neutralizing antibody attenuated the bFGF-induced REX1 expression by SHEDs. In conclusion, bFGF enhanced REX1 expression by SHEDs via the FGFR and Akt signaling pathways. Moreover, IL-6 participated in the bFGF-induced REX1 expression in SHEDs. J. Cell. Biochem. 118: 1480-1488, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 74%

Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Basic Fibroblast Growth Factor Regulates REX1 Expression Via IL‐6 In Stem Cells Isolated From Human Exfoliated Deciduous Teeth

Nowwarote

Sukarawan²,

Pavasant

et al. 2016

J of Cellular Biochemistry

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bFGF and JAGGED1 regulate alkaline phosphatase expression and mineralization in dental tissue-derived mesenchymal stem cells

Osathanon¹,

Nowwarote

Manokawinchoke

et al. 2013

J. Cell. Biochem.

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Basic fibroblast growth factor (bFGF) and Notch signaling play critical roles in various cell behaviors. Here, we investigated the influence of bFGF and Notch signaling in alkaline phosphatase (ALP) expression and mineralization process in human periodontal ligament-derived mesenchymal stem cells (PDLSCs) and stem cells isolated from human exfoliated deciduous teeth (SHEDs). PDLSCs and SHEDs were cultured in osteogenic medium supplemented with bFGF or on the immobilized Notch ligands, JAGGED1. The ALP mRNA and protein expression were measured by quantitative reverse transcriptase polymerase chain reaction and enzymatic activity assay, respectively. Mineral deposition was determined using alizarin red S staining. The results showed that the addition of bFGF resulted in the decrease of ALP mRNA expression and enzymatic activity. In addition, the attenuation of mineralization was noted. These phenomenons were blocked by the addition of a fibroblast growth factor receptor inhibitor (SU5402) or a MEK inhibitor (PD98059). Interestingly, bFGF supplementation also decreased the Notch signaling component mRNA levels. Thus, to evaluate effect of Notch signaling in mineralization process, PDLSCs and SHEDs were exposed to JAGGED1 modified surface. The ALP mRNA and protein expression were significantly upregulated and the mineral deposition was markedly increased. These results could be reversed by the addition of a γ-secretase inhibitor. In addition, bFGF could attenuate the Notch-signaling-induced mineralization in both PDLSCs and SHEDs. These results suggest that mineralization was enhanced by Notch signaling but attenuated by bFGF signaling. This knowledge can be further utilized to control PDLSCs and SHEDs mineralization for tissue regeneration purpose.

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High Glucose Condition Suppresses Neurosphere Formation by Human Periodontal Ligament‐Derived Mesenchymal Stem Cells

Sawangmake¹,

Pavasant

Chansiripornchai³

et al. 2014

J of Cellular Biochemistry

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D-Glucose serves many roles in cellular functions, but its role in human periodontal ligament-derived mesenchymal stem cells (hPSLSCs) is yet unknown. Here, the roles of high glucose concentration on neurogenic differentiation by hPDLSCs were investigated. Two-stage neurogenic induction protocol was employed. Cells were maintained in normal neurogenic induction medium, high glucose condition, or high mannose condition. The results showed that high glucose attenuated neurosphere formation efficiency by hPDLSCs in terms of morphology, neurogenic marker expression, without a deleterious effect on cell viability. Contrastingly, neurosphere-derived cells matured in high glucose condition exhibited normal neuronal characteristics compared to the control. During neurosphere formation in high glucose, glucose transporters (GLUTs) mRNA levels were significantly decreased, corresponding with the deprivation of cellular glucose uptake. Further, a glucose uptake inhibitor, cytochalasin B, was used to confirm the deleterious effects of glucose uptake deprivation during neurosphere formation. The results demonstrated that deprivation of glucose uptake attenuated neurosphere formation efficiency by hPDLSCs. Together, the results illustrated that high glucose condition attenuated the efficiency of neurosphere formation but not neuronal maturation, which may occur through the downregulation of GLUTs and the reduction of glucose uptake.

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IL‐6 regulated stress‐induced Rex‐1 expression in stem cells from human exfoliated deciduous teeth

Abstract: These results suggest the role of mechanical stress in the regulation of stemness via IL-6-Rex-1 interaction.

Cited by 19 publications

References 60 publications

Basic Fibroblast Growth Factor Regulates REX1 Expression Via IL‐6 In Stem Cells Isolated From Human Exfoliated Deciduous Teeth

Basic Fibroblast Growth Factor Regulates REX1 Expression Via IL‐6 In Stem Cells Isolated From Human Exfoliated Deciduous Teeth

bFGF and JAGGED1 regulate alkaline phosphatase expression and mineralization in dental tissue-derived mesenchymal stem cells

High Glucose Condition Suppresses Neurosphere Formation by Human Periodontal Ligament‐Derived Mesenchymal Stem Cells

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