<scp>L</scp>-Carnitine Pretreatment Protects Slow-Twitch Skeletal Muscles in a Rat Model of Ischemia–Reperfusion Injury

Demirel, Mert; Kaya, Burak; Çerkez, Cem; Ertunç, Mert; Sara, Yıldırım

doi:10.1177/1538574413496481

Cited by 6 publications

(3 citation statements)

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“…Woitaske et al observed an increased mitochondrial swelling and disrupted sarcoplasmic reticulum in the glycolytic EDL as compared to soleus muscle (Woitaske and McCarter, 1998 ). Accordingly, IR- induced contractile dysfunction was recently shown to be reduced in soleus as compared to EDL (Demirel et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…After 4 h ischemia and 2 h reperfusion, the force-frequency curve was more altered in extensor digitorum longus (EDL) than in soleus . Further, resistance to fatigue was lower in EDL than in soleus (Demirel et al, 2013 ). Impaired fatigue resistance and contraction were also observed after IR in gastrocnemius (Zhang et al, 2017 ) and accordingly, proteins implicated in muscle contraction are known to be targets for ROS (Beckendorf and Linke, 2015 ), which are largely implicated in IR pathophysiology (increased lipid peroxidation and oxidized proteins; Avci et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

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Muscles Susceptibility to Ischemia-Reperfusion Injuries Depends on Fiber Type Specific Antioxidant Level

et al. 2017

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Muscle injury resulting from ischemia-reperfusion largely aggravates patient prognosis but whether and how muscle phenotype modulates ischemia-reperfusion-induced mitochondrial dysfunction remains to be investigated. We challenged the hypothesis that glycolytic muscles are more prone to ischemia-reperfusion-induced injury than oxidative skeletal muscles. We therefore determined simultaneously the effect of 3 h of ischemia induced by aortic clamping followed by 2 h of reperfusion (IR, n = 11) on both gastrocnemius and soleus muscles, as compared to control animals (C, n = 11). Further, we investigated whether tempol, an antioxidant mimicking superoxide dismutase, might compensate a reduced defense system, likely characterizing glycolytic muscles (IR-Tempol, n = 7). In the glycolytic gastrocnemius muscle, as compared to control, ischemia-reperfusion significantly decreased mitochondrial respiration (−30.28 ± 6.16%, p = 0.003), increased reactive oxygen species production (+79.15 ± 28.72%, p = 0.04), and decreased reduced glutathione (−28.19 ± 6.80%, p = 0.011). Less deleterious effects were observed in the oxidative soleus muscle (−6.44 ± 6.30%, +4.32 ± 16.84%, and −8.07 ± 10.84%, respectively), characterized by enhanced antioxidant defenses (0.63 ± 0.05 in gastrocnemius vs. 1.24 ± 0.08 μmol L−1 g−1 in soleus). Further, when previously treated with tempol, glycolytic muscle was largely protected against the deleterious effects of ischemia-reperfusion. Thus, oxidative skeletal muscles are more protected than glycolytic ones against ischemia-reperfusion, thanks to their antioxidant pool. Such pivotal data support that susceptibility to ischemia-reperfusion-induced injury differs between organs, depending on their metabolic phenotypes. This suggests a need to adapt therapeutic strategies to the specific antioxidant power of the target organ to be protected.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Muscles Susceptibility to Ischemia-Reperfusion Injuries Depends on Fiber Type Specific Antioxidant Level

et al. 2017

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“…Ischemia-reperfusion (I/R) injury is an occurrence that is observed very widespread in orthopedic traumatology and reconstructive surgery and can have detrimental effects on all tissues and organs involved. This event negatively influences the result of surgical procedures for the management of amputated or severely traumatized extremities in traumatology and patients who had a reconstructive surgery (10).…”

Section: Introductionmentioning

confidence: 99%

Effect of iloprost on contractile impairment and mitochondrial degeneration in ischemia-reperfusion of skeletal muscle

et al. 2018

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Purpose Acute lower extremity ischemia is still a main cause of mortality and morbidity in orthopedic traumatology and reconstructive surgery. In acute lower extremity ischemia, the skeletal muscles are the tissues that are the most vulnerable to ischemia. The aim of this study was to evaluate the effects of iloprost (IL) therapy on skeletal muscle contractile impairment and mitochondrial degeneration in an acute lower extremity ischemia-reperfusion rat model. Main Methods Forty Wistar albino rats were randomly divided into a control group and four experimental groups. Experimental groups were either subjected to 2 h of lower extremity ischemia followed by a 4-h reperfusion period or to 4 h of ischemia followed by an 8-h reperfusion period. Except for the animals in the control group, all animals received IL (1 ng/kg/min) or saline (1 ml/kg) by intraperitoneal infusion for 10 min immediately before reperfusion. At the end of the recording of skeletal muscle electrical activity and contractility, all rats were sacrificed by decapitation and muscle samples of lower extremity were immediately harvested for histopathologic analyses. Results After ischemia-reperfusion, a breakdown in the force-frequency curves of extensor digitorum longus muscle was observed, showing the diminished muscle contractility. However, IL significantly improved muscle contractility following injury induced by 2 h of ischemia followed by a 4-h reperfusion period. In addition, IL partially ameliorated mitochondrial degeneration in the muscle cells of ischemia groups. Conclusion This study indicates that immediate IL therapy repairs muscle damage especially after 2 h of ischemia and 4 h of reperfusion and therefore that IL improves contractile function.

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Potential protective effects of l-carnitine against neuromuscular ischemia-reperfusion injury: From experimental data to potential clinical applications

Moghaddas

Dashti‐Khavidaki

2016

Clinical Nutrition

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L-Carnitine Pretreatment Protects Slow-Twitch Skeletal Muscles in a Rat Model of Ischemia–Reperfusion Injury

Cited by 6 publications

References 20 publications

Muscles Susceptibility to Ischemia-Reperfusion Injuries Depends on Fiber Type Specific Antioxidant Level

Muscles Susceptibility to Ischemia-Reperfusion Injuries Depends on Fiber Type Specific Antioxidant Level

Effect of iloprost on contractile impairment and mitochondrial degeneration in ischemia-reperfusion of skeletal muscle

Potential protective effects of l-carnitine against neuromuscular ischemia-reperfusion injury: From experimental data to potential clinical applications

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