<scp>RKIP</scp>
            mediates autoimmune inflammation by positively regulating
            <scp>IL</scp>
            ‐17R signaling

Lin, Wenlong; Wang, Ning; Zhou, Kangxing; Su, Fasheng; Yu, Junsheng; Shou, Jianan; Liu, Huan; Ma, Chao; Qian, Youchun; Wang, Kai; Wang, Xiaojian

doi:10.15252/embr.201847502

Cited by 5 publications

(4 citation statements)

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“…Consistent with these findings, the KEGG enrichment analysis showed that the α-PDPN treatment inhibited the IL-17 signaling pathway. The binding of IL-17 to the IL-17R activates the IL-17R signaling pathway and ultimately triggers the expression of proinflammatory chemokines (CCL2, CCL7, and CCL20) (23). Similarly, the effect of α-PDPN on IL-17 signaling pathway was reported in an in-vitro experimental study, which showed that the preincubation of isolated peripheral blood mononuclear cells with α-PDPN reduced the secretion of IL-17 and IL-1ꞵ (24).…”

Section: Discussionmentioning

confidence: 80%

Transcriptome sequencing analysis revealed the molecular mechanism of podoplanin neutralization inhibiting ischemia/reperfusion-induced microglial activation

Qian¹,

Qian

Yang³

et al. 2022

Ann Transl Med

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Background: Anti-podoplanin antibody (α-PDPN, clone 8.1.1) reduces microglia-mediated inflammation and decreases cerebral infarct volume in mice with stroke. However, the molecular mechanism by which this occurs is unknown. This study sought to systematically analyze the molecular mechanism of α-PDPN treatment on ischemia/reperfusion (I/R)-injured microglia.Methods: Microglia BV2 cells were pre-cultured with α-PDPN and then exposed to oxygen-glucose deprivation and reoxygenation (OGD-R) insult. The differentially expressed genes (DEGs) underwent a transcriptome sequencing technology analysis, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Quantitative real-time polymerase chain reaction (PCR) was performed to confirm the transcriptional expression of some DEGs. Results:The results showed that α-PDPN downregulated 338 genes and upregulated 340 genes in the BV2 cells. The GO items of the downregulated DEGs mainly involved biological processes, such as the response to the interferon (IFN), lipopolysaccharide-mediated signaling pathway, and the regulation of cell chemotaxis and migration. The upregulated molecular function mainly involved glucocorticoid-receptor binding. Further, the KEGG pathway analysis indicated that the enriched categories for the upregulated DEGs mainly involved the adenosine triphosphate (ATP) binding cassette transporters. However, the interleukin-17 signaling pathway, IFN signaling pathway, tumor necrosis factor signaling pathway, transforming growth factor beta (TGF-ꞵ) signaling pathway, nucleotide-binding and oligomerization domain (NOD)-like receptor signaling pathway, cytokine-cytokine receptor interaction, and chemokine signaling pathway were downregulated by the α-PDPN treatment.Conclusions: Numerous inflammation-related signaling pathways were regulated by the α-PDPN treatment in the OGD-R injured BV2 cells. This study provided further insights into the protective mechanism of α-PDPN treatment in ischemic stroke.

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Section: Discussionmentioning

confidence: 80%

Transcriptome sequencing analysis revealed the molecular mechanism of podoplanin neutralization inhibiting ischemia/reperfusion-induced microglial activation

Qian¹,

Qian

Yang³

et al. 2022

Ann Transl Med

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“…In CNS, IL-17RA is expressed on all kinds of resident cells, including microglia, astrocytes, neurons and endothelial cells [43,50,51]. IL-17RA signaling on microglia has been reported by many studies and became the target for drugs [43,52]. There was no intracellular enzymatic activity that has been found on IL-17RA and its complexes [53].…”

Section: Discussionmentioning

confidence: 99%

Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH

Liu

Deng

Ding

et al. 2023

Experimental Neurology

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“…IL-17 signalling pathway mediates apoptosis and autophagy, and is related to immune and inflammatory activities (Sun et al, 2018). IL-17 protein activates the IL-17 signalling pathway after binding to its receptor IL-17r, and further binding with adaptin Act1 to activate MAPK and NF-KB pathways to induce tissue damage (Lin et al, 2019). Eukaryotic ribosome synthesis pathway, RNA transport pathway and RNA degradation pathway are related to the expression of cellular genetic information, RNA synthesis and translation (Schilders, van Dijk, Raijmakers, & Pruijn, 2006) (Nazar, 2004;Suzuki, Izumi, & Ohno, 2010).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of deoxynivalenol‐induced apoptosis of sow ovarian granulosa cell

Yang

Wang

Zhang

et al. 2020

Reprod Domestic Animals

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Litter size is one of the most important economic traits in pig production. Recent studies identified that deoxynivalenol (DON), a widespread toxin in fodder, was associated with animal prolificacy. However, the underlying mechanisms have not yet been completely elucidated. Here, we used porcine ovary granulosa cells (pGCs) as a vector to establish DON concentration–time models and performed cell morphology and transcriptome analysis to identify and analyse the effects of DON on reproductive performance in swine. The results showed that DON can induce morphological changes and apoptosis of pGCs, while inhibiting cell proliferation. Moreover, these effects of DON on pGCs were dose‐dependent. After treatment of pGCs with different concentrations of DON, the percentage of cells in S phase and G2/M phase increased. RNA‐seq analyses revealed 5,937 differentially expressed genes, of which 1995 were down‐regulated and 3,942 were up‐regulated after DON treatment. KEGG enrichment analysis indicated important metabolic pathways such as IL‐17 signalling pathway, eukaryotic ribosome synthesis pathway, RNA transport pathway and RNA degradation. Based on our results, we speculate that the effects of DON are related to the DNA damage process. Our study provides novel insights and a foundation to further understand the effect of DON on swine prolificacy.

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RKIP mediates autoimmune inflammation by positively regulating IL ‐17R signaling

Cited by 5 publications

References 0 publications

Transcriptome sequencing analysis revealed the molecular mechanism of podoplanin neutralization inhibiting ischemia/reperfusion-induced microglial activation

Transcriptome sequencing analysis revealed the molecular mechanism of podoplanin neutralization inhibiting ischemia/reperfusion-induced microglial activation

Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH

Transcriptional analysis of deoxynivalenol‐induced apoptosis of sow ovarian granulosa cell

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