Screening and evaluation of antioxidant, antimicrobial, cytotoxic, thrombolytic and membrane stabilizing properties of the methanolic extract and solvent-solvent partitioning effect of Vitex negundo Bark

Khan, Shamsuddin Sultan; Syeed, Sharif Hossain; Uddin, Hanif; Akter, Lucky; Ullah, Asmat; Jahan, Suria; Rashid, Harunur

doi:10.1016/s2222-1808(13)60090-0

Cited by 19 publications

(11 citation statements)

References 9 publications

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“…Chowdhury et al (2010) showed antibacterial activity of two compounds isolated from leaves of V. negundo. The bark extract of V. negundo exhibited antimicrobial activity (Khan et al, 2013).…”

Section: Discussionmentioning

confidence: 98%

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Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens – A Comparative Study

Prashith¹,

Manasa²,

Poornima³

et al. 2013

Sci. Technol. Arts Res. J.

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Article InformationThe present study was carried out to estimate the content of total phenolics, flavonoids, negundoside and agnuside and to determine antibacterial, cytotoxic and antioxidant efficacy of methanol extract of two varieties of Vitex negundo namely V. negundo var. negundo (Vnvn) and V. negundo var. purpurascens (Vnvp). Total phenolic and flavonoid content of leaf extracts were estimated by Folin-Ciocalteau reagent and Aluminium chloride colorimetric estimation method respectively. Negundoside and agnuside content were estimated using HPLC. Antibacterial activity of both the extracts was tested by Agar well diffusion assay. Cytotoxicity of extract was evaluated by Brine shrimp lethality bioassay. Antioxidant activity of extracts was determined by DPPH free radical scavenging and Ferric reducing assay. The content of total phenolics and flavonoid were higher in extract of Vnvp. Negundoside and agnuside contents were high in Vnvn and Vnvp respectively. Leaf extract of Vnvp was shown to display marked antibacterial, cytotoxic and antioxidant activity when compared with extract of Vnvn. Both the varieties of V. negundo showed marked antibacterial, cytotoxic and antioxidant activity. The extract of Vnvp displayed marked bioactivities than that of extract of Vnvn. The presence of high phenolic and flavonoid content could be attributed to the marked bioactivities of Vnvp.

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Section: Discussionmentioning

confidence: 98%

“…In another study, Chowdhury et al (2010) showed cytotoxic activity in terms of brine shrimp mortality of two compounds isolated from leaves of V. negundo. Crude methanol extract and solvent fractions of bark of V. negundo were shown to exhibit marked cytotoxic effect in terms of mortality of brine shrimp larvae (Khan et al, 2013).…”

Section: Discussionmentioning

confidence: 98%

Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens – A Comparative Study

Prashith¹,

Manasa²,

Poornima³

et al. 2013

Sci. Technol. Arts Res. J.

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“…Natural products offer a promising alternative for treatment of infectious diseases. Plants, plant-based formulations, and purified compounds from plants have shown inhibitory activity against a variety of pathogenic bacteria including drug-resistant strains [9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Interest in natural antioxidants is triggered due to suspected negative effects of synthetic antioxidants. Botanicals are proven to be promising resources of agents with antioxidant potential [12,16,[18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Insecticidal, Antibacterial, and Antiradical Activity of Nicotiana Plumbaginifolia Viv. (Solanaceae)

Tr¹,

Mr²,

Hc³

2017

Asian J Pharm Clin Res

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Objectives: Nicotiana plumbaginifolia Viv. belongs to the family Solanaceae. The present study was carried out to determine insecticidal, antibacterial, and antiradical activity of aerial parts of N. plumbaginifolia.Methods: Extraction was carried out by maceration process using methanol. Insecticidal activity was assessed, in terms of larvicidal effect, against II and IV instar larvae of Aedes aegypti. Antibacterial activity was evaluated against a panel of 7 bacteria by agar well diffusion assay. Antiradical activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Results:The extract was found to cause dose-dependent mortality of larvae of A. aegypti. The lethal concentration 50 value of extract for II and IV instar larvae was found to be 0.41 and 0.99 mg/ml, respectively. Extract was effective in inhibiting all bacteria. Gram-positive bacteria displayed marked susceptibility when compared to Gram-negative bacteria. Bacillus cereus and Escherichia coli were susceptible to highest and least extent, respectively. Extract scavenged both DPPH radicals and ABTS radicals dose dependently. Extract scavenged ABTS radicals more efficiently (inhibitory concentration [IC 50 ] value 13.51 µg/ml) when compared to DPPH radicals (IC 50 value 17.43 µg/ml). Conclusions:The plant N. plumbaginifolia appears to be a promising resource for developing agents with insecticidal, antibacterial, and antiradical activity. The observed bioactivities could be attributed to the presence of phytochemicals which are to be isolated, characterized, and subjected for bioactivity determinations. The plant can be used to prevent arboviral diseases, infectious diseases, and oxidative damage.

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“…It is a region of non-specific immune reaction that happens in response to any kind of bodily damage, is a complicated biological response of vascular tissues to deleterious stimuli 10,11 . Membrane stabilising agents are helpful to scale back the hyperexcitability of nerves 12 .…”

Section: Introductionmentioning

confidence: 99%

THROMBOLYTIC AND MEMBRANE STABILIZING PROPERTIES OF METHANOLIC EXTRACTS OF AVICENNIA MARINA LEAVES & BARKS

Uddin¹,

Millat²,

Ira³

et al. 2016

Int. Res. J. Pharm.

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The present study was commenced to judge ethnomedicinal worth of the plant. We tried to analyze in vitro thrombolytic and membrane stabilising activities of methanolic extracts of Avicennia marina. Crude methanolic extracts of A. marina leaves & barks at numerous conc. were used for in vitro thrombolytic and membrane stabilising activities. Just in case of thrombolytic study, it absolutely was dose dependently accumulated, wherever 10 mg/ml concentration most importantly showed 13.22% lysis of clot (p<0.001) by in vitro clot lysis assay technique. Streptokinase was used as standard & water was treated as negative management. Crude methanolic extracts of A. marina dose dependently increased in membrane stabilising study, whereas 10 mg/ml concentration most importantly showed 10.11% & 22.23% inhibition of haemolysis severally by both hypotonic solution and heat induced haemolysis of RBC membrane. Acetyl salicylic acid was used as standard in membrane stabilising study.

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Screening and evaluation of antioxidant, antimicrobial, cytotoxic, thrombolytic and membrane stabilizing properties of the methanolic extract and solvent-solvent partitioning effect of Vitex negundo Bark

Cited by 19 publications

References 9 publications

Antibacterial, Cytotoxic and Antioxidant Potential of <i>Vitex Negundo Var. Negundo</i> and <i>Vitex Negundo</i> Var. <i>Purpurascens</i> – A Comparative Study

Antibacterial, Cytotoxic and Antioxidant Potential of <i>Vitex Negundo Var. Negundo</i> and <i>Vitex Negundo</i> Var. <i>Purpurascens</i> – A Comparative Study

Insecticidal, Antibacterial, and Antiradical Activity of Nicotiana Plumbaginifolia Viv. (Solanaceae)

THROMBOLYTIC AND MEMBRANE STABILIZING PROPERTIES OF METHANOLIC EXTRACTS OF AVICENNIA MARINA LEAVES & BARKS

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