Screening and identification of xylanase-producing bacteria from Thai soils

Kinegam, Saowapar; Tanasupawat, Somboon; Akaracharanya, Ancharida

doi:10.2323/jgam.53.57

Cited by 9 publications

(5 citation statements)

References 45 publications

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“…The locations of the homologues of MTS1_00475 of the 13 isolated strains used to create the phylogenetic tree were investigated. The following bacteria and associated locations were identified: three bacteria related to plants (root spheres of plants, potato leaves, and a symptomatic Veronica plant; Morohoshi et al, 2011 ; Corretto et al, 2016 ; Savory et al, 2017 ); two bacteria isolated from the aquatic environment (marine animals like Dictyopodium moseleyi and salt lakes; Kageyama et al, 2007 ; Mishra et al, 2018 ); five bacteria derived from soil ( Collins et al, 1983 ; Park et al, 2006 ; Kinegam et al, 2007 ; Zhang et al, 2010 ); one bacterium isolated from subsurface sediments ( Brown et al, 2012 ); one bacterium isolated from oil reservoirs ( Schippers et al, 2005 ); one bacterium isolated from Corn steep liquor ( Yokota et al, 1993 ). Microbacterium spp.…”

Section: Resultsmentioning

confidence: 99%

Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract

et al. 2022

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The radionuclide isotopes (134Cs and 137Cs) of Cesium (Cs), an alkali metal, are attracting attention as major causes of radioactive contamination. Although Cs+ is harmful to the growth of plants and bacteria, alkaliphilic bacterium Microbacterium sp. TS-1, isolated from a jumping spider, showed growth even in the presence of 1.2 M CsCl. The maximum concentration of Cs+ that microorganisms can withstand has been reported to be 700 mM till date, suggesting that the strain TS-1 is resistant to a high concentration of Cs ions. Multiple reports of cesium ion-resistant bacteria have been reported, but the detailed mechanism has not yet been elucidated. We obtained Cs ion-sensitive mutants and their revertant mutants from strain TS-1 and identified a Cs ion resistance-related gene, MTS1_00475, by performing SNP analysis of the whole-genome sequence data. When exposed to more than 200 mM Cs+ concentration, the intracellular Cs+ concentration was constantly lowered by MTS1_00475, which encodes the novel low-affinity Cs+/H+ antiporter. This study is the first to clarify the mechanism of cesium resistance in unexplained cesium-resistant microorganisms. By clarifying the new cesium resistance mechanism, it can be expected to be used as a bioremediation tool for treating radioactive Cs+ contaminated water.

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Section: Resultsmentioning

confidence: 99%

Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract

et al. 2022

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“…A study [60] reported xylanase production from Bacillus thermoleovorans strain K-3D and Bacillus flavothermus strain LB3A. Xylanase production capability was also reported in Bacillus, Cupriavidus, Microbacterium, Pseudoxanthomonas, and Rhodococcus [61]. Microbacterium species produced enzymes involved in cellulose and xylan degradation [62][63][64][65].…”

Section: Discussionmentioning

confidence: 98%

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Okeke

2010

Appl Biochem Biotechnol

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Diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. Inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. In an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, purified 19 monocultures and evaluated their cellulolytic-xylanolytic potential. Five selected isolates (DB1, DB2, DB7, DB8, and DB13) were used to compose a defined consortium and characterized by 16S ribosomal RNA gene sequence analysis. Nucleotide sequence blast analysis revealed that DB1, DB2, DB7, DB8, and DB13 were respectively similar to Pseudoxanthomonas byssovorax (99%), Microbacterium oxydans (99%), Bacillus sp. (99%), Ochrobactrum anthropi (98%), and Klebsiella trevisanii (99%). The isolates produced an array of cellulolytic-xylanolytic enzymes (filter paper cellulase, β-glucosidase, xylanase, and β-xylosidase), and significant activities were recorded in 30 min. Isolates DB1 and DB2 displayed the highest filter paper cellulase: 27.83 and 31.22 U mg⁻¹, respectively. The highest β-glucosidase activity (18.07 U mg⁻¹) was detected in the culture of isolate DB1. Isolate DB2 produced the highest xylanase activity (103.05 U mg⁻¹), while the highest β-xylosidase activity (7.72 U mg⁻¹) was observed with DB13. Use of microbial consortium in bioprocessing of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, adsorption, and requirement for high amounts of enzymes in direct use of enzymes.

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“…When compared to the data presented in literature, screening and isolation of xylanase producer from Zea mays (plant) was easier and quicker than that from soil (Panbangred et al 1983, Avcioglu et al 2005, Rawashadeh et al 2005, Xu et al 2005, Kinegam et al 2007. In this study, we isolated two xylanase producers B. pumilus isolates.…”

Section: Xylanolytic Microorganism Isolationmentioning

confidence: 76%

“…(Degrassie et al 2003, Avcioglu et al 2005 Xylanase producers were mostly isolated from soil. Soil based isolation needs screening of many soil samples and microorganisms (Panbangred et al 1983, Avcioglu et al 2005, Rawashdeh et al 2005, Kinegam et al 2007, culture enrichment steps (Sanchez et al 2005, Xu et al 2005) and serial dilutions (Rawashdeh et al 2005). Endopyhtes are defined as organisms inhabit inside of leaves, braches and roots without harming the plant (Hawkswoth et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Endopyhtic and Xylanolytic Bacillus pumilus Strains from Zea mays

YAŞINOK¹,

Ayşegül;ŞAHİN²,

İffet;HABERAL³

et al. 2008

Tarım Bilimleri Dergisi

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In this study, two endophytic xylanolytic bacteria, named M1 and M2, were isolated from surface sterilized Zea mays stern and leaf, respectively. Isolates were identified as Bacillus pumilus. Microorganisims showed different morphology on agar plates. Xylanase production level and profile varied between isolates, as well. Maximum xylanase production level of 188.0 ± 20.0 and 5.6 ± 1.1 U/ml were achieved by Bacillus pumilus M1 and Bacillus pumilus M2 in a liquid medium containing 3% corn cobs as a sole carbon source and inducer, respectively. Isolates produced very low level of cellulase in crude enzyme extract. B. pumilus M1 xylanase was partially characterized. Enzyme had a maximum activity at pH 8.0 and 65°C, seemed proper for pulp and paper industry, and required further characterization. In conclusion, this study indicated that inside part of Zea mays is a novel and good source for isolating xylanase producers. After detailed characterization, such enzymes could be used in various applications in paper and pulp, textile, food and feed industries.Key Words: Bacillus pumilus, endopyhte, plant, xylan, xylanase producers. Zea mays'dan Endofitik ve Ksilanolitik Bacillus pumilus Suş ları n ı n İzolasyonuÖz: Bu çal ışmada, yüzey sterilizasyonu yap ı lm ış Zea mays gövde ve yapraklar ı ndan, s ı ras ı ile M1 ve M2 olarak isimlendirilen iki tane endofitik ksilanolitik bakteri izole edilmi ştir. Izolatlar Bacillus pumilus olarak tan ı mlanm ıştı r. Mikroorganizmalar agar üzerinde farkl ı morfoloji sergilemi ştir. Izolatlar aras ı nda, ksilanaz üretim seviyesi ve profilinde de farkl ı l ı klar bulunmaktad ı r. İçinde %3 m ı s ı r koçan ı n ı tek karbon kaynağı olarak bulunduran s ı v ı besi yerinde, Bacillus pumilus Mi ve Bacillus pumilus M2 s ı ras ı ile maksimum ksilanaz aktivitesi olan 188.0 ± 20.0 ve 5.6 t 1.1 U/mi değ erlerine ula şabilmi ştir. İzolatlar, kaba fermentasyon özütünde çok dü ş ük miktarda selülaz üretmi ştir. B. pumilus M1 ksilanaz ı k ı smi karakterize edilmi ştir. Enzim pH 8.0 ve 65°C'de maksimum aktivite götermi ş , ka ğı t beyazlatma endüstrisine uygun görülmü ş ve ileri derecede karakterizasyona ihtiyaç duymu ştur. Sonuç olarak, bu çal ış ma Zea mays' ı n iç k ı sm ı n ı n, ksilanaz üreticilerinin izolasyonu için yeni ve iyi bir kaynak oldu ğ unu göstermi ştir. Detayl ı tan ı mlama ard ı ndan bu enzimler, ka ğı t, tekstil, g ı da ve yem endüstrisinde çe ş itli uygulamalarda değerlendirilebilmesi mümkündür.

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Screening and identification of xylanase-producing bacteria from Thai soils

Cited by 9 publications

References 45 publications

Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract

Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Isolation of Endopyhtic and Xylanolytic Bacillus pumilus Strains from Zea mays

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