Screening antagonistic yeasts against citrus green mold and the possible biocontrol mechanisms of <i>Pichia galeiformis</i> (<scp>BAF03</scp>)

Chen, Ou; Yi, Lanhua; Deng, Lili; Ruan, Changqing; Zeng, Kaifang

doi:10.1002/jsfa.10407

Cited by 48 publications

(23 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…Previously, it has been repeatedly shown that P. kudriavzevii (and other representatives of this genus) has a strong antagonistic effect on both mold and phytopathogenic fungi. [33][34][35] It has been suggested that this species could be utilized for practical purposes in the protection of agricultural products from mold. The compost itself (even BSFL-free, after removal the larvae) could possibly have an anti-fungal effect, providing a positive effect on plant growth and health.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dramatic effect of black soldier fly larvae on fungal community in a compost

et al. 2021

View full text Add to dashboard Cite

BACKGROUND Black soldier fly larvae (BSFL) offer very good prospects for the production of cheap and high‐quality dietary protein. This insect is able to consume low‐quality substrates, including food waste. The properties and safety of the compost obtained are largely determined by its microbiome. However, while the bacterial component of the BSFL‐compost microbiome has been much studied, little is known about its fungal component. In plant‐based rearing substrates both the biomass and the metabolic activity of fungi often exceed the biomass and the activity of prokaryotes. So, the purpose of this study was to investigate the fungal community of the compost produced by BSF larvae reared on a food waste substrate. RESULTS Community structure was determined by metabarcoding of the ITS (internal transcribed spacer) region. Species composition and abundance were determined using the plating technique and subsequent identification of the isolated pure cultures. It was found that the primary mycobiome of the food waste substrate that was used consisted of 19 families, represented mainly by phytopathogenic and endophytic genera. Larva incubation led to the complete elimination of all mycelial fungi from the resulting compost. The final mycobiome consisted of only two yeast species, Pichia kudriavzevii and Diutina rugosa, with a total abundance of 1.2 × 107 CFU g−1. CONCLUSIONS The rearing of BSFL led to the complete elimination of mycelial fungi from its feed substrate. The final compost lacked harmful fungi, including molds. This information may be crucial for BSF compost utilization. The phenomenon was also an interesting aspect of zoomicrobial interactions in nature and agriculture. © 2021 Society of Chemical Industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dramatic effect of black soldier fly larvae on fungal community in a compost

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Pichia galeiformis has been reported with efficient control of postharvest green mold in citrus through competition for space and nutrients as well as production of volatile organic compounds . However, the effect of P.…”

Section: Introductionmentioning

confidence: 99%

Pichia galeiformis Induces Resistance in Postharvest Citrus by Activating the Phenylpropanoid Biosynthesis Pathway

Chen

Deng

Ruan

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

This study aimed to investigate the effect of Pichia galeiformis on disease resistance and elucidate the changes in phenylpropane biosynthesis treated by P. galeiformis in postharvest citrus. The results showed that P. galeiformis reduced the disease incidence and lesion diameters without direct contact with the pathogen Penicillium digitatum. Transcriptome analysis revealed that phenylpropanoid biosynthesis was triggered by P. galeiformis. Genes encoding phenylpropanoid biosynthesis were upregulated, including phenylalanine ammonia-lyase (PAL), 4-coumaroyl-CoA ligase (4CL), cinnamate-4-hydroxylase (C4H), peroxidase (POD), cinnamyl alcohol dehydrogenase (CAD), O-methyltransferase, and hydroxyl cinnamoyl transferase. Moreover, P. galeiformis increased the activity of PAL, 4CL, C4H, POD, polyphenol oxidase, and CAD in citrus pericarp. In addition, P. galeiformis treated citrus displayed higher levels of total phenolic compounds, flavonoid, and lignin and higher amounts of ferulic and sinapic acid. In conclusion, these results suggested that P. galeiformis could induce resistance through modulating the pathway of phenylpropanoid biosynthesis in postharvest citrus.

show abstract

“…The VOC 2-phenylethanol is reported as a common inhibitory compound produced by several biocontrol yeasts including Debaryomyces nepalensis , Wickerhamomyces anomalus , Metschnikowia pulcherrima , Saccharomyces cerevisiae , and Pichia manshurica (formerly, Pichia galeiformis ) ( Zhou et al, 2018 ; Contarino et al, 2019 ; Chen et al, 2020 ). In this study, we found a strong inhibitory effect of 2-phenylethanol on germination and mycelium growth of C. inversicolor and P. roqueforti at 10 –3 mol/L.…”

Section: Discussionmentioning

confidence: 99%

“…This indicates that efficient mold inhibition relied on additive or synergistic effects of the yeast inhibition factors such as competition for nutrients and space as well as production of soluble compounds or VOCs ( Núñez et al, 2015 ). Similarly, P. manshurica has been shown to colonize and amplify quickly in citrus wounds and further to produce eight VOCs, including acetic acid and 2-phenylethanol during growth, which inhibited growth of Penicillium digitatum , causing citrus green mold ( Chen et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Debaryomyces hansenii Strains Isolated From Danish Cheese Brines Act as Biocontrol Agents to Inhibit Germination and Growth of Contaminating Molds

et al. 2021

View full text Add to dashboard Cite

The antagonistic activities of native Debaryomyces hansenii strains isolated from Danish cheese brines were evaluated against contaminating molds in the dairy industry. Determination of chromosome polymorphism by use of pulsed-field gel electrophoresis (PFGE) revealed a huge genetic heterogeneity among the D. hansenii strains, which was reflected in intra-species variation at the phenotypic level. 11 D. hansenii strains were tested for their ability to inhibit germination and growth of contaminating molds, frequently occurring at Danish dairies, i.e., Cladosporium inversicolor, Cladosporium sinuosum, Fusarium avenaceum, Mucor racemosus, and Penicillium roqueforti. Especially the germination of C. inversicolor and P. roqueforti was significantly inhibited by cell-free supernatants of all D. hansenii strains. The underlying factors behind the inhibitory effects of the D. hansenii cell-free supernatants were investigated. Based on dynamic headspace sampling followed by gas chromatography-mass spectrometry (DHS-GC-MS), 71 volatile compounds (VOCs) produced by the D. hansenii strains were identified, including 6 acids, 22 alcohols, 15 aldehydes, 3 benzene derivatives, 8 esters, 3 heterocyclic compounds, 12 ketones, and 2 phenols. Among the 71 identified VOCs, inhibition of germination of C. inversicolor correlated strongly with three VOCs, i.e., 3-methylbutanoic acid, 2-pentanone as well as acetic acid. For P. roqueforti, two VOCs correlated with inhibition of germination, i.e., acetone and 2-phenylethanol, of which the latter also correlated strongly with inhibition of mycelium growth. Low half-maximal inhibitory concentrations (IC50) were especially observed for 3-methylbutanoic acid, i.e., 6.32–9.53 × 10–5 and 2.00–2.67 × 10–4 mol/L for C. inversicolor and P. roqueforti, respectively. For 2-phenylethanol, a well-known quorum sensing molecule, the IC50 was 1.99–7.49 × 10–3 and 1.73–3.45 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. For acetic acid, the IC50 was 1.35–2.47 × 10–3 and 1.19–2.80 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. Finally, relative weak inhibition was observed for 2-pentanone and acetone. The current study shows that native strains of D. hansenii isolated from Danish brines have antagonistic effects against specific contaminating molds and points to the development of D. hansenii strains as bioprotective cultures, targeting cheese brines and cheese surfaces.

show abstract

Screening antagonistic yeasts against citrus green mold and the possible biocontrol mechanisms of Pichia galeiformis (BAF03)

Cited by 48 publications

References 53 publications

Dramatic effect of black soldier fly larvae on fungal community in a compost

Dramatic effect of black soldier fly larvae on fungal community in a compost

Pichia galeiformis Induces Resistance in Postharvest Citrus by Activating the Phenylpropanoid Biosynthesis Pathway

Debaryomyces hansenii Strains Isolated From Danish Cheese Brines Act as Biocontrol Agents to Inhibit Germination and Growth of Contaminating Molds

Contact Info

Product

Resources

About