Screening Autoantibody Profiles in Systemic Rheumatic Disease with a Diagnostic Protein Microarray That Uses a Filtration-Assisted Nanodot Array Luminometric Immunoassay (NALIA)

McBride, Jeffrey D.; Gabriel, Francis G.; Fordham, J. L. A.; Kolind, Torsten; Barcenas-Morales, Gabriela; Isenberg, David; Swana, M.; Delves, Peter J.; Lund, Torben; Cree, Ian A.; Roitt, Ivan M.

doi:10.1373/clinchem.2007.098418

Cited by 18 publications

(9 citation statements)

References 20 publications

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“…The use of these tests requires knowledge of their fundamental aspects and also of the clinical classification criteria of each disorder in order to contribute to an appropriate diagnosis [5] , [6] .…”

Section: Introductionmentioning

confidence: 99%

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Soto

Hernández-Becerril

Perez-Chiney

et al. 2015

Results in Immunology

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Introduction: Determination of antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) is usually the initial test for the diagnosis of systemic rheumatic diseases (SRD). Assigning predictive values to positive and negative results of the test is vital because lack of knowledge about ANAs and their usefulness in classification criteria of SRD leads to inappropriate use. Methods: Retrospective study, ANA tests requested by different specialties, correlation to patients' final diagnosis. Results: The prevalence of autoimmune disease was relatively low in our population yielding a low PPV and a high NPV for the ANA test. 40% of the patients had no clinical criteria applied prior to test. Coexistence of two or more autoimmune disorders affects prevalence and predictive values. Conclusion: Application of the test after careful evaluation for clinical criteria remarkably improves the positive likelihood ratio for the diagnosis.

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Section: Introductionmentioning

confidence: 99%

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Soto

Hernández-Becerril

Perez-Chiney

et al. 2015

Results in Immunology

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“…The distinctive feature of the plate is the immobilisation of probes on the backside of the membrane, and not inside the well as usually done (Perrin et al, 2003). To our knowledge, this non-conventional design was only mentioned once in the literature for chemiluminescent immunoassays (McBride et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Multipurpose high-throughput filtering microarrays (HiFi) for DNA and protein assays

Goff

Desmet

Brès

et al. 2010

Biosensors and Bioelectronics

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“…To our knowledge, that spotting approach has been mentioned only once in the literature for chemiluminescent immunoassays. 29 The association of HiFi plate innovative technological developments with the sample preparation multiplexing leads to a performing platform for high-throughput genotyping.…”

Section: Resultsmentioning

confidence: 99%

Robust, High-Throughput Solution for Blood Group Genotyping

et al. 2010

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With the concomitant increase of blood transfusions and safety rules, there is a growing need to integrate high-throughput and multiparametric assays within blood qualification centers. Using a robust and automated solution, we describe a new method for extended blood group genotyping (HiFi-Blood 96) bringing together the throughput possibilities of complete automation and the microarray multiplexed analysis potential. Our approach provides a useful resource for upgrading blood qualification center facilities. A set of six single-nucleotide polymorphisms (SNPs) associated with clinically important blood group antigens (Kell, Kidd, Duffy, and MNS systems) were selected and the corresponding genotyping assays developed. A panel of 293 blood samples was used to validate the approach. The resulting genotypes were compared to phenotypes previously determined by standard serologic techniques, and excellent correlations were found for five SNPs out of six. For the Kell, Kidd, Duffy, and MNS3/MNS4 systems, high matching percentages of 100%, 98.9%, 97.7%, and 97.4% were obtained, respectively, whereas a concordance percentage of 83.3% only was attained for the MNS1/MNS2 polymorphism.

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Screening Autoantibody Profiles in Systemic Rheumatic Disease with a Diagnostic Protein Microarray That Uses a Filtration-Assisted Nanodot Array Luminometric Immunoassay (NALIA)

Abstract: BACKGROUND:We developed a cost-efficient modular system for multiplex analysis of the multiple autoantibodies that characterize systemic rheumatoid diseases.

Cited by 18 publications

References 20 publications

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

Multipurpose high-throughput filtering microarrays (HiFi) for DNA and protein assays

Robust, High-Throughput Solution for Blood Group Genotyping

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