Screening for Lactobacillus plantarum Strains That Possess Organophosphorus Pesticide-Degrading Activity and Metabolomic Analysis of Phorate Degradation

Abstract

This work performed a large scale assessment for organophosphorus pesticides (OPPs) degradation activity of 121 Lactobacillus (L.) plantarum strains. Six L. plantarum strains (P9, IMAU80110, IMAU40100, IMAU10585, IMAU10209, and IMAU80070) were found to possess high capacity of degrading three commonly used OPPs, namely dimethoate, phorate, and omethoate; and they were selected for more detailed characterization. Moreover, the three OPPs were mainly detected in the culture supernatants but not in the cell extracts, further confirming that the OPPs were degraded rather than absorbed by the cells. Among the six selected strains, P9 was most tolerant to gastrointestinal juices and bile. We thus used ultra-high performance liquid chromatography electron spray ionization coupled with time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) to generate the metabolomic profiles of the strain P9 growing in MRS medium with and without containing phorate. By using orthogonal partial least squares discriminant analysis, we identified some potential phorate-derived degradative products. This work has identified novel lactic acid bacteria resources for application in pesticide degradation. Our results also shed light on the phorate degradation mechanism by L. plantarum P9.

Fermentation Characteristics of Lactococcus lactis subsp. lactis Isolated From Naturally Fermented Dairy Products and Screening of Potential Starter Isolates

“…The bacteria were collected by centrifugation at 3500 r/min for 15 min, washed twice in phosphate-buffered saline (PBS), and then diluted to concentrations of 10 7 and 10 8 colony forming units (CFUs) per mL by PBS. After a further 48 h at 37 • C the cells were counted using the pouring method and suspensions placed at 4 • C prior to use in fermentation runs (Li et al, 2018).…”

Identification of Genome Sequences of Polyphosphate-Accumulating Organisms by Machine Learning

“…L. plantarum JBE245 is a member of L. plantarum which is isolated from malolactic fermentation of apple juice (Heo and Uhm, 2017 ). Some work reported that L. plantarum strains possess organophosphorus pesticide-degrading activity (Li et al, 2018 ). Therefore, L. plantarum JBE245 was probably an undiscovered PAOs.…”

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

“…to mitigate the harmful effects of pesticides and pathogens. The basis for this is several-fold, but the most discernible benefit of LAB supplementation is that it can reduce pesticide absorption via degradation (Islam et al, 2010;Lénárt et al, 2013;Zhang et al, 2016;Li et al, 2018) or by sequestering ingested pesticides, thereby allowing them to pass through the digestive tract rather than be absorbed (Trinder et al, 2016). In other model organisms, LAB have been shown to reduce toxicity and have a protective effect to the host (Bouhafs et al, 2015;Bagherpour Shamloo et al, 2016), thus establishing a basis for future studies to investigate this potential in honey bees.…”