The results of studies on the possibility of applying the biological product Bacillus subtilis for the protection of vineyards from powdery mildew are presented. Meteorological conditions in 2016 and 2018 contributed to the epiphytotic prevalence of powdery mildew in the agrocenosis of Rostov region. In 2015, 2017 and 2019 its prevalence was moderate. The use of biological preparation against powdery mildew showed the prospects of its application. With a moderate degree of disease prevalence, the use of a biological preparation instead of chemical fungicides is possible in all phases of a growing season. It inhibits the pathogen’s development, contributes to conservation of plants’ productivity and reducing the pesticide load. In years with epiphytotic prevalence of powdery mildew it is desirable to apply fungicides during pre-veraison, and biopreparation – up to the phase of technical maturity. Such application will contribute to obtaining a quantitative and qualitative yield, and will also reduce the toxicological effect of chemical fungicides used in the first half of a growing season.