Screening Napier grass accessions for resistance to Napier grass stunt disease using the loop-mediated isothermal amplification of DNA (LAMP)

Wamalwa, N.I.E.; Midega, Charles A. O.; Ajanga, S.; Omukunda, N.E.; Muyekho, Francis N.; Asudi, George O.; Mulaa, M.

doi:10.1016/j.cropro.2017.02.005

Cited by 14 publications

(3 citation statements)

References 26 publications

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“…Many cultivars of Napier grass, and hybrids with pearl millet ( Cenchrus purpureus (Schumach.) Morrone × Cenchrus americanus (L.) Morrone), have been developed in different parts of the world for their high yield potential, broad adaptability, resistance to diseases and feed quality traits [ 14 ]. Despite the information on the adaptability of Napier grass to short-term drought stress, there have been no genotypes identified that can tolerate and produce biomass to support the availability of feed for livestock in the longer dry season conditions of tropical environments.…”

Section: Introductionmentioning

confidence: 99%

Productivity and Feed Quality Performance of Napier Grass (Cenchrus purpureus) Genotypes Growing under Different Soil Moisture Levels

Habte¹,

Gari²,

Muktar³

et al. 2022

Plants

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In the semi-arid and arid environments of Sub-Sharan Africa, forage availability throughout the year is insufficient and highly limited during the dry seasons due to limited precipitation. Thus, the identification of drought stress-tolerant forage cultivars is one of the main activities in forage development programs. In this study, Napier grass (Cenchrus purpureus), an important forage crop in Eastern and Central Africa that is broadly adapted to produce across tropical environments, was evaluated for its water use efficiency and production performance under field drought stress conditions. Eighty-four Napier grass genotypes were evaluated for their drought stress tolerance from 2018 to 2020 using agro-morphological and feed quality traits under two soil moisture stress regimes during the dry season, i.e., moderate (MWS) and severe (SWS) water stress conditions, and under rainfed conditions in the wet season (wet). Overall, the results indicated the existence of genotype variation for the traits studied. In general, the growth and productivity of the genotypes declined under SWS compared to MWS conditions. High biomass-yielding genotypes with enhanced WUE were consistently observed across harvests in each soil moisture stress regime. In addition, the top biomass-yielding genotypes produced the highest annual crude protein yield, indicating the possibility of developing high-feed-quality Napier grass genotypes for drought stress environments.

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Section: Introductionmentioning

confidence: 99%

Productivity and Feed Quality Performance of Napier Grass (Cenchrus purpureus) Genotypes Growing under Different Soil Moisture Levels

Habte¹,

Gari²,

Muktar³

et al. 2022

Plants

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“…Several studies have been directed towards the characterization and evaluation for forage yield, feed quality, and resistance to the major diseases affecting Napier grass [16]. From the ILRI genebank collection, some genotypes shown to be promising for high biomass yield and nutritional quality have been identified and utilized by national programs, including in Ethiopia and Kenya [17][18][19]. Due to the impacts of climate change in East and Central Africa (ECA) and the potential risks from the emergence and spread of pests and diseases [20], the selection and development of new forage varieties with desirable traits that fit with the climatic conditions and production systems of ECA needs continuous assessment of a large number of genotypes.…”

Section: Introductionmentioning

confidence: 99%

Forage Performance and Detection of Marker Trait Associations with Potential for Napier Grass (Cenchrus purpureus) Improvement

Habte¹,

Muktar²,

Abdena³

et al. 2020

Agronomy

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The evaluation of forage crops for adaptability and performance across production systems and environments is one of the main strategies used to improve forage production. To enhance the genetic resource base and identify traits responsible for increased feed potential of Napier grass, forty-five genotypes from Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Brazil, were evaluated for forage biomass yield and feed nutritional quality in a replicated trial under wet and dry season conditions in Ethiopia. The results revealed significant variation in forage yield and feed nutritional qualities among the genotypes and between the wet and dry seasons. Feed fiber components were lower in the dry season, while crude protein, in vitro organic matter digestibility, and metabolizable energy were higher. Based on the cumulative biomass and metabolizable energy yield, top performing genotypes were identified that are candidates for future forage improvement studies. Furthermore, the marker-trait association study identified diagnostic single nucleotide polymorphisms (SNP) and SilicoDArT markers and potential candidate genes that could differentiate high biomass yielding and high metabolizable energy genotypes in the collection.

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“…These were included with the aim of enhancing the genetic diversity and generating information useful for designing breeding strategies for Napier grass. Each collection represented a different region of the world; for example, the ILRI and KALRO collections were mostly from Africa 18 , 38 , while the EMBRAPA materials were mainly from Brazil 39 , 40 . Similarly, most of the genotypes from ICRISAT’s collection had an Asian origin 41 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of global Napier grass (Cenchrus purpureus) collections reveals high genetic diversity among genotypes with some redundancy between collections

Muktar,

Bizuneh,

Anderson

et al. 2023

Sci Rep

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Genetic diversity amongst genotypes of several Napier grass collections was analyzed and compared with the diversity in a set of open pollinated progeny plants. A total of 114,881 SNP and 46,293 SilicoDArT genome-wide markers were generated on 574 Napier grass genotypes. Of these, 86% of the SNP and 66% of the SilicoDArT markers were mapped onto the fourteen chromosomes of the Napier grass genome. For genetic diversity analysis, a subset of highly polymorphic and informative SNP markers was filtered using genomic position information, a maximum of 10% missing values, a minimum minor allele frequency of 5%, and a maximum linkage-disequilibrium value of 0.5. Extensive genetic variation, with an average Nei’s genetic distance value of 0.23, was identified in the material. The genotypes clustered into three major and eleven sub-clusters with high levels of genetic variation contained both within (54%) and between (46%) clusters. However, we found that there was low to moderate genetic differentiation among the collections and that some overlap and redundancy occurred between collections. The progeny plants were genetically diverse and divergent from the germplasm collections, with an average FST value of 0.08. We also reported QTL regions associated with forage biomass yield based on field phenotype data measured on a subset of the Napier grass collections. The findings of this study offer useful information for Napier grass breeding strategies, enhancement of genetic diversity, and provide a guide for the management and conservation of the collections.

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Screening Napier grass accessions for resistance to Napier grass stunt disease using the loop-mediated isothermal amplification of DNA (LAMP)

Cited by 14 publications

References 26 publications

Productivity and Feed Quality Performance of Napier Grass (Cenchrus purpureus) Genotypes Growing under Different Soil Moisture Levels

Productivity and Feed Quality Performance of Napier Grass (Cenchrus purpureus) Genotypes Growing under Different Soil Moisture Levels

Forage Performance and Detection of Marker Trait Associations with Potential for Napier Grass (Cenchrus purpureus) Improvement

Analysis of global Napier grass (Cenchrus purpureus) collections reveals high genetic diversity among genotypes with some redundancy between collections

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