Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production

Li, Feng; Xie, Yingjie; Gao, Xiang; Shan, Mingxu; Sun, Changchao; Niu, Yan D.; Shan, Anshan

doi:10.1016/j.ejbt.2020.09.001

Cited by 43 publications

(37 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As per the results displayed in Figure 4, in acidic pH 4.0-5.5 conditions, the enzyme showed good stability, whereas its stability in highly alkaline (pH 8.0-10.0) conditions was limited. These results are consistent with other cellulolytic Bacillus spp., which showed the optimal pH for cellulase activity towards acidic conditions (Rawat and Tewari, 2012;Li et al, 2020).…”

Section: Temperature and Ph Effectmentioning

confidence: 98%

Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

Malik

Javed

2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Microbial cellulases have become the mainstream biocatalysts due to their complex nature and widespread industrial applications. The present study reports the partial purification and characterization of cellulase from Bacillus subtilis CD001 and its application in biomass saccharification. Out of four different substrates, carboxymethyl cellulose, when amended as fermentation substrate, induced the highest cellulase production from B. subtilis CD001. The optimum activity of CMCase, FPase, and amylase was 2.4 U/ml, 1.5 U/ml, and 1.45 U/ml, respectively. The enzyme was partially purified by (NH4)2SO4 precipitation and sequenced through LC-MS/MS. The cellulase was found to be approximately 55 kDa by SDS-PAGE and capable of hydrolyzing cellulose, as confirmed by zymogram analysis. The enzyme was assigned an accession number AOR98335.1 and displayed 46% sequence homology with 14 peptide-spectrum matches having 12 unique peptide sequences. Characterization of the enzyme revealed it to be an acidothermophilic cellulase, having an optimum activity at pH 5 and a temperature of 60°C. Kinetic analysis of partially purified enzyme showed the Km and Vmax values of 0.996 mM and 1.647 U/ml, respectively. The enzyme activity was accelerated by ZnSO4, MnSO4, and MgSO4, whereas inhibited significantly by EDTA and moderately by β-mercaptoethanol and urea. Further, characterization of the enzyme saccharified sugarcane bagasse, wheat straw, and filter paper by SEM, ATR-FTIR, and XRD revealed efficient hydrolysis and structural modifications of cellulosic materials, indicating the potential industrial application of the B. subtilis CD001 cellulase. The findings demonstrated the potential suitability of cellulase from B. subtilis CD001 for use in current mainstream biomass conversion into fuels and other industrial processes.

show abstract

Section: Temperature and Ph Effectmentioning

confidence: 98%

Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

Malik

Javed

2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The distillers grains are dried and crushed to a size below 60 mesh. Distillers grains were collected from a winery in Yibin, China; peptone, yeast extract, NaCl, (NH 4 ) 2 SO 4 , KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 ·H 2 O, and FeCl 3 were purchased from Kelong Chemical Co., Ltd.(Chengdu, China); Ferulic acid and ethyl4-hydroxy-3-methoxycinnamate were purchased from Yuanye Biotechnology Co. Ltd. (Shanghai, China); The strains used in this study were isolated from the intestines and feces of bamboo rat, Yibin, China, and 16S Rdna was used to classify and identify these bacteria: Z28 , Klebsiella pneumoniae JZE (JZE) , Bacillus mycoides JEF (JZF) , Bacillus cereus JZ3 (JZ3) , Bacillus velezensis G1 (G1 ) , Siccibacter colletis G2 ( G2 ), and Bacillus subtilis strain G6 (G6) [ 17 , 18 , 19 , 20 ]. Isolated strains were grown in LB medium, kept at 4 °C, and subcultured periodically for experiments.…”

Section: Methodsmentioning

confidence: 99%

The Combined Cultivation of Feruloyl Esterase-Producing Strains with CMCase and Xylanase-Producing Strains Increases the Release of Ferulic Acid

Zhang

Jiang

et al. 2022

Microorganisms

View full text Add to dashboard Cite

Feruloyl esterase (FAE)-producing micro-organisms to obtain ferulic acid (FA) from natural substrates have good industrial prospects, and the synergistic effect of multiple bacteria can better improve the yield of FA. In this study, on the premise of the synergistic effect of FAE, hemicellulose, and cellulase, the key strain Klebsiella oxytoca Z28 with FAE was combined with CMCase and Xylanase-producing strains to produce FA. The combination of strains with higher FA production are Klebsiella oxytoca Z28, Klebsiella pneumoniae JZE, Bacillus velezensis G1, and their FA production can reach 109.67 μg/g, which is 15% higher than that of single bacteria. To explore the effects of temperature, Ph, inoculum amount, distillers grains concentration and fermentation time on the FAE activity of the combination of strains in the fermentation process, and determined that temperature, Ph, and fermentation time were the main influencing factors and optimized through orthogonal design. The optimized fermentation conditions are 34 °C, Ph 8.0, and fermentation days for 6 days, the FAE activity can reach 270.78 U/L, and the FA yield of the combined strain is 324.50 μg/g, which is 200% higher than that of single-strain fermentation.

show abstract

“…[36] Trichoderma reesei RUT C30 [37] Talaromyces pinophilus EMU [38] Trichoderma reesei [39] Pestalotiopsis microspora TKBRR [40] Talaromyces emersonii [41] Trichoderma orientalis EU7-22 [42] Trichoderma reesei [43] Aspergillus niger [44] Trichoderma harzianum EM0925 [45] Humicola grisea var. thermoidea [46] Bacteria Bacillus pseudomycoides [47] Bacillus velezensis [48] Bacillus amyloliquefaciens FW2 [49] Lactobacillus plantarum RI 11 [50] Burkholderia sp. [51] In the literature, many research articles have been published, which identify and emphasize many fungal species as best producers of cellulases and hemicellulases.…”

Section: Fungimentioning

confidence: 99%

“…After pretreatment, a centrifugation step reveals the recovery of remaining DDGS, which can be sold as the byproduct of the process (Figure 3). After the initial upstream processing steps, fungal fermentation can be completed with the optimized media and fermentation conditions [48,50]. For example, in the study conducted by the authors with one of the selected fungal strains, the addition of nitrogen sources (11 g/L yeast extract, 5 g/L peptone and 2 g/L ammonium sulfate) increased the enzyme production by many folds [72].…”

Section: Integration Of 1g and 2g Bioethanol Refineriesmentioning

confidence: 99%

Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

2022

View full text Add to dashboard Cite

First-generation (1G) bioethanol is one of the most used liquid biofuels in the transport industry. It is generated by using sugar- or starch-based feedstocks, while second-generation (2G) bioethanol is generated by using lignocellulosic feedstocks. Distillers’ dried grains with solubles (DDGS) is a byproduct of first-generation bioethanol production with a current annual production of 22.6 million tons in the USA. DDGS is rich in fiber and valuable nutrients contents, which can be used to produce lignocellulolytic enzymes such as cellulases and hemicellulases for 2G bioethanol production. However, DDGS needs a pretreatment method such as dilute acid, ammonia soaking, or steam hydrolysis to release monosaccharides and short-length oligosaccharides as fermentable sugars for use in microbial media. These fermentable sugars can then induce microbial growth and enzyme production compared to only glucose or xylose in the media. In addition, selection of one or more suitable microbial strains, which work best with the DDGS for enzyme production, is also needed. Media optimization and fermentation process optimization strategies can then be applied to find the optimum conditions for the production of cellulases and hemicellulases needed for 2G bioethanol production. Therefore, in this review, a summary of all such techniques is compiled with a special focus on recent findings obtained in previous pieces of research conducted by the authors and by others in the literature. Furthermore, a comparison of such techniques applied to other feedstocks and process improvement strategies is also provided. Overall, dilute acid pretreatment is proven to be better than other pretreatment methods, and fermentation optimization strategies can enhance enzyme production by considerable folds with a suitable feedstock such as DDGS. Future studies can be further enhanced by the technoeconomic viability of DDGS as the on-site enzyme feedstock for the manufacture of second-generation bioethanol (2G) in first-generation (1G) ethanol plants, thus bridging the two processes for the efficient production of bioethanol using corn or other starch-based lignocellulosic plants.

show abstract

Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production

Cited by 43 publications

References 25 publications

Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

The Combined Cultivation of Feruloyl Esterase-Producing Strains with CMCase and Xylanase-Producing Strains Increases the Release of Ferulic Acid

Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

Contact Info

Product

Resources

About