Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer

Agustien, Anthoni; Santoso, Putra; Sari, Nespy Permata; Fathya, Annisa; Nasir, Nasril; Rilda, Yetria; Djamaan, Akmal

doi:10.20546/ijcmas.2017.612.457

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Koloni yang tumbuh dengan bentuk berbeda dengan koloni lainnya dianggap sebagai isolat berbeda. Koloni bakteri dimurnikan sampai diperoleh isolat murni pada media NA yang baru [7].…”

Section: Isolasi Bakteri Endofitunclassified

Uji Aktivitas Antibakteri Dari Ekstrak Bakteri Endofit Yang Diisolasi Dari Kulit Jeruk Nipis

Ruslan

Ismed

Nabila³

2022

J Sains Farm Klin

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Kulit Jeruk nipis (Citrus aurantifolia Swingle) merupakan bagian tanaman yang banyak digunakan untuk pengobatan dengan berbagai aktivitas farmakologis. Namun masih sedikit diketahui kajian tentang bakteri endofit yang terkait dengan kulit buah tanaman ini. Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari kulit buah tanaman ini serta menguji aktivitas dalam mengambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Methicilin-Resistant Staphylococcus aureus (MRSA). Sebanyak enam Isolat bakteri berhasil diisolasi lalu difermentasi dalam media Nutrient Broth (NB). Hasil fermentasi kemudian diekstraksi menggunakan pelarut etil asetat. Ekstrak kental masing-masing isolat diujikan aktivitas antibakterinya menggunakan metode difusi Kirby-Bauer. Berdasarkan hasil pengukuran zona hambat menunjukkan bahwa ekstrak dari isolat 3 dan isolat 5 memiliki diameter hambat kuat >10 mm. Hasil identifikasi fitokimia secara kualitatif menunjukkan bahwa ekstrak dari isolat 3 menujukkan adanya kandungan alkaloid dan flavonoid sedangkan isolat 5 teridentifikasi senyawa alkaloid, flavonoid dan polifenol. Hasil identifikasi penamaan bakteri endofit menunjukkan bahwa isolat 3 merupakan Bacillus velezensis strain JS25R dan isolat 5 merupakan Staphylococcus sp. Ekstrak dari bakteri endofit ini terbukti memiliki aktivitas terhadap bakteri patogen dan potensial untuk dilakukan isolasi dan pengembangan terhadap senyawa metabolit sekunder yang memiliki aktivitas tersebut.

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“…Koloni yang tumbuh dengan bentuk berbeda dengan koloni lainnya dianggap sebagai isolat berbeda. Koloni bakteri dimurnikan sampai diperoleh isolat murni pada media NA yang baru [7].…”

Section: Isolasi Bakteri Endofitunclassified

Uji Aktivitas Antibakteri Dari Ekstrak Bakteri Endofit Yang Diisolasi Dari Kulit Jeruk Nipis

Ruslan

Ismed

Nabila³

2022

J Sains Farm Klin

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“…Sampling: A sampling of leaves from the three types of betel plants studied, (green betel, red betel and forest betel) was conducted by purposive random sampling method, samples were taken from the location Jambi City Forest Park Area. Leaf samples were taken that looked healthy, not young leaves or old leaves, each leaf sample was taken with as many as ten leaves from each clump 4 .…”

Section: Methodsmentioning

confidence: 99%

“…The manufacture of antibiotics in Indonesia is highly dependent on imports of medicinal raw materials, now the import value is still high (90%). In efforts to reduce imports, the government has determined that the use of the countryʼs biodiversity is gradually used as raw materials for the manufacture of antibiotics, the production of which is through fermentation 4,5 . Resource search endophytic microbes get a lot of attention because there are still many that have not been characterized, so their potential has not been maximized, especially in our country, Indonesia 6 .…”

Section: Introductionmentioning

confidence: 99%

Exploration of Antibiotics-Producing Endophytic Bacteria Isolates from Betel Leaves in Jambi City Forest Park

Arzita¹,

Fitriani²,

Fathia³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: Sources of antibiotics that are cheap and easy to obtain are by exploiting endophytic microbes from betel plants because betel plant extracts are known to produce anti-microbial compounds. This research aimed to obtain isolates of antibiotic producer endophilic bacteria from betel leaves in Jambi city forest park. Materials and Methods: This research consists of several stages, sterilization of the collected betel leaf surface, isolation and purification of endophytic bacterial isolates, screening of antibiotic-producing bacteria and characterization of endophytic bacteria. Results: There were 3 types of betel plants found in the area, namely: Piper betle L. (green betel), Piper crocatum Ruiz and Pav (red betel) and Piper aduncum L. (forest betel). In red betel leaves, 9 types of endophytic bacterial isolates were obtained, while only 1 isolate was found in each green betel and forest betel leaf. Of these 11 isolates, 9 of them produced antibiotics. However, only 6 isolates were potential since they had a halo zone bigger than 10 mm for E. coli (gram-negative) and S. aureus (gram-positive). Among these 6 isolates, 5 isolates were from red leaves with isolate codes of BESKJ-m2, BESHKJ-m3, BESHKJ-m4, BESHKJ-m5, BESHKJ-m6 and 1 isolate from forest betel leaves with code of BESHKJ-s1. Conclusion: From the control positive test using chloramphenicol, it was known that the endophilic bacteria had a broad spectrum. The bacterial colonies were white and had an elevation on the surface of the media with flat to irregular margins. The catalase and Gram stain tests showed that the bacteria were Gram-positive.

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“…The ability of endophytic microbes to produce secondary metabolites according to their host plants is a very large and reliable opportunity to produce secondary metabolites from endophytic microbes isolated from their host plants 3 . endophytic bacteria BES-5 was isolated from Piper betle in the HPPB forest area have the ability to produce secondary metabolites in the form of antibiotics that are narrow spectrum and include moderate strong category antibiotics 4 . Increasing the ability of bacteria to produce antibiotics with higher inhibition of microorganisms can be done by optimizing the fermentation process so that optimum conditions for antibiotic production include bacterial growth curve profile and antibiotic activity, type and concentration from inducers to antibiotic activities, effects of temperature, pH and agitation on the production media on the antibiotic activity of the concentration effect of the inoculum on antibiotic activity, the effect of type and concentration of carbon and nitrogen sources and trace element to antibiotic activity 3 .…”

Section: Introductionmentioning

confidence: 99%

Improving Antibiotic Activities From Endophytic Isolate Bes-5 Gram Positive Bacteria

Agustien¹,

Nasir²,

Alamsjah³

et al. 2019

Int. Res. J. Pharm.

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Antibiotics are metabolites of microorganisms that can kill or inhibit the growth of other microorganisms. This study aims to increase the inhibition of antibiotics produced from endophytic BES-5 positive Gram bacteria. The increasing of antibiotic activity is carried out by optimizing the production of antibiotics from bacteria. Antibiotic activity was determined by the Kirby method with E. coli as test bacteria. The results showed to obtain antibiotics with very strong category characteristics, with optimum conditions for incubation temperature of 33 0 C; pH of production media 7.5; agitation of 150 rpm; 5% inoculum; 3% soaking corn water as inducer, 5% inoculum, 0.5% glucose as carbon source. The optimum condition besides increasing the inhibition also accelerates the production of antibiotics.

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Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer

Cited by 4 publications

References 8 publications

Uji Aktivitas Antibakteri Dari Ekstrak Bakteri Endofit Yang Diisolasi Dari Kulit Jeruk Nipis

Uji Aktivitas Antibakteri Dari Ekstrak Bakteri Endofit Yang Diisolasi Dari Kulit Jeruk Nipis

Exploration of Antibiotics-Producing Endophytic Bacteria Isolates from Betel Leaves in Jambi City Forest Park

Improving Antibiotic Activities From Endophytic Isolate Bes-5 Gram Positive Bacteria

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