Screening of N-acylneuraminic acids in serum and tissue specimens of mouse C57BI with Lewis' lung cancer by high-performance liquid chromatography

Makatsori, E.; Fermani, K; Aletras, Alexis J.; Karamanos, Nikos K.; Tsegenidis, T.

doi:10.1016/s0378-4347(98)00150-9

Cited by 23 publications

(13 citation statements)

References 24 publications

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“…Furthermore, Sia-containing glycoconjugates are common epitopes in tumors (Bhavanandan and Furukawa, 1995). Numerous studies have demonstrated the correlation between high levels of sialic acid with different types of cancer (Makatsori et al, 1998;Sillanauke et al, 1999;Narayanan, 1994;Zhang et al, 2002). Neu5Ac is present in the majority of sialic acid-containing tumor markers in humans, even though emerging data suggests that Neu5Gc is also correlated with certain human cancers (Kawai, 1991;Tzanakakis et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Expression and distribution of N‐acetyl and N‐glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Tzanakakis

Nikitovic

Katonis

et al. 2007

Biomedical Chromatography

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N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are the dominant sialic acids (Sia) in mammals usually found in the non-reducing terminal of oligosaccharide side chains in glycoproteins and glycolipids. Their expression and distribution pattern have been correlated both with the malignant phenotype and tumor grade of human cancers. The aim of the present study was to determine by reversed-phase HPLC method the amounts of Neu5Ac and Neu5Gc as well as their distribution among the culture media and cell surface of MG-63 and Saos-2 human osteosarcoma cell lines of high and low metastatic potential. It was determined that MG-63 cells produce up to 5-fold more total sialic acid as compared with the Saos 2 cells. Neu5Ac accounts for ca 60% of the total sialic acids secreted by MG-63 cells, whereas Neu5Gc is the predominant sialic acid present on the MG-63 cell membrane. Saos 2 cells secrete considerable amounts of Neu5Ac to culture media. The obtained data indicate that the human osteosarcoma cells express both forms of Sia-containing glycoconjugates; the differences in the amounts of each of the two major Sia types and their distribution may be related to their differences in morphology and/or metastatic potentials.

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Section: Introductionmentioning

confidence: 99%

Expression and distribution of N‐acetyl and N‐glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Tzanakakis

Nikitovic

Katonis

et al. 2007

Biomedical Chromatography

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“…Often HPLC or CE after derivatization with different reagents are employed, e.g. HPLC or CE after per-Obenzoylation [5,6], CE of 2-aminoacridone-derivates [7] or HPLC with DMB-derivatives [8,9]. Examples for analytical methods without derivatization are analyses by RP HPLC [10], RP ion-pair HPLC [11] and CE [12,13] each combined with UV detection; unfortunately these methods lack sensitivity and selectivity.…”

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confidence: 99%

Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry

Ortner

Buchberger

2008

Electrophoresis

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A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 microg/mL and 6 microg/mL, respectively.

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“…This alteration may result in deregulation of cell growth and proliferation. There is a correlation between high levels of Sia among different types of cancers [26][27][28][29][30] . Neu5Ac is present in the majority of Sia-containing tumours in humans, even though emerging data suggests that Neu5Gc is also correlated with certain human cancers 31,32 .…”

Section: Discussionmentioning

confidence: 99%

Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

Phitak¹,

Klangjorhor²,

Pothacharoen³

et al. 2017

ScienceAsia

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Sialic acids, which are members of a family of 9-carbon carboxylated sugars, usually occupy the terminal position of the glycan chains of glycoconjugates and play an important role in various biological processes. Nacetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are the dominant sialic acids in mammals, whereas deaminoneuraminic acids (KDN) can be detected in trace amounts. The increase of sialic acid levels has been reported in relation to the pathology and aggressiveness of cancer. Here we used a fluorometric-HPLC method to investigate the level and distribution of Neu5Ac, Neu5Gc, and KDN in osteosarcoma cells isolated from 7 patients and compare them with those in osteoblasts isolated from 3 patients with other bone diseases. Both cell types expressed all three sialic acids with abundances Neu5Ac > Neu5Gc > KDN. Total sialic acid levels in osteosarcoma cells were twice that in osteoblast cells. Only cell-associated Neu5Ac and Neu5Gc levels were higher, but not secreted forms. However, cell-associated and secreted KDN levels were not significantly different among those two groups. This suggests that the level and distribution of sialic acids in osteosarcoma cells were different from those in normal osteoblast cells. Thus their level, type, and distribution may be related to the pathology of osteosarcoma.

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Screening of N-acylneuraminic acids in serum and tissue specimens of mouse C57BI with Lewis' lung cancer by high-performance liquid chromatography

Cited by 23 publications

References 24 publications

Expression and distribution of N‐acetyl and N‐glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Expression and distribution of N‐acetyl and N‐glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry

Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

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