Screening of Rice Resources against Rice Black-Streaked Dwarf Virus and Mapping of Resistant QTL

Abstract

Rice black-streaked dwarf virus (RBSDV) disease become epidemic in Jiangsu and Zhejiang provinces. To screen resistant germplasms and discover new resistance genes/QTLs against RBSDV, we evaluated the resistance to RBSDV in 311 japonica varieties in field test. The results showed that no variety was immune to RBSDV. The disease rate of 24 main japonica varieties grown in Jiangsu province at present was 10.0%-29.0% and that of 71.5% varieties in 287 japonica varieties popularized in Jiangsu before was between 10.0% and 30.0%. Furthermore, quantitative trait loci (QTL) analysis was conducted by using 162 recombinant inbred lines (RILs), which derived from a cross between Guichao 2, a susceptible indica variety, and Koshihikari, a japonica variety with resistance to RBSDV. RBSDV resistances were evaluated using natural infection methods by scoring the disease rating. One putative QTL (qRBSDV3) controlling RBSDV resistance was mapped between the marker RM7 and RM5748 on chromosome 3, which explained 17.1% of the total phenotypic variation with LOD score of 5.4. The positive resistant effect contributed from Koshihikari. Further analysis revealed that the lines harboring the alleles of qRBSDV3 exhibited significantly increased resistance to RBSDV. The results should be very useful for breeding new RBSDV-resistant cultivars by marker-assisted selection (MAS).

Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice (Oryza sativa L.)

“…Among the five RBSDVD resistance QTLs identified in the present study, four QTLs, including qRBSDV3 WR24 , qRBSDV6 WR24 , qRBSDV9 WR24 and qRBSDV11 WR24, were also located in the same “hot spot” on chromosome 3, 6, 9 and 11. For example, qRBSDV3 WR24 was located in the same chromosome interval with qRBSDV3 (Wang et al., ) and qRBSDV3 9194 (Sun et al., ). qRBSDV6 WR24 and qRBSDV11 WR24 shared the same flank SSR marker with qRBSDV6 and qRBSDV‐11 (Sun et al., ; Zhou et al., ).…”

“…qRBSDV3 WR24 , qRBSDV6 WR24 , qRBSDV7 WR24 , qRBSDV9 WR24 and qRBSDV11 WR24 , were detected. Previous studies found that the QTLs conferring RBSDVD resistance were frequently and repeatedly detected on the chromosome 3, 6, 7, 9 and 11 (Duan et al., ; Li et al., ; Sun et al., ; Wang et al., ; Zheng et al., ; Zhou et al., ). Among the five RBSDVD resistance QTLs identified in the present study, four QTLs, including qRBSDV3 WR24 , qRBSDV6 WR24 , qRBSDV9 WR24 and qRBSDV11 WR24, were also located in the same “hot spot” on chromosome 3, 6, 9 and 11.…”

Screening of rice (Oryza sativa) cultivars for resistance to rice black streaked dwarf virus using quantitative PCR and visual disease assessment

“…Among 311 japonica cultivars screened by Wang et al . () to evaluate the level of RBSDD resistance, the disease incidence on plants of 24 widely grown cultivars ranged from 10 to 29%, and no variety was immune. Because the specificity, stability and durability of resistance can change, controlling the virus disease is a challenging task (Lecoq et al ., ).…”

Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines

“…In recent years, damage caused by SBPH feeding has increased, and serious yield reductions have occurred as a consequence [1–3]. Rice viral diseases, such as rice stripe virus (RSV) and rice black-streaked dwarf virus (RBSDV), transmitted by SBPH, often cause major yield losses [4,5]. Rice stripe disease is one of the most serious rice diseases in China.…”

“…However, varieties with single resistance genes are always at risk of their resistance being overcome by new strains of virus. There were no reported sources of high resistance to RBSDV, until recently [5,19]. It is therefore necessary to find additional sources of resistance to RSV and other viruses vectored by SBPH.…”