Screening of the most relevant parameters for method development in ultra-high performance hydrophilic interaction chromatography

Periat, Aurélie Claudine; Debrus, Benjamin; Rudaz, Serge; Guillarme, Davy

doi:10.1016/j.chroma.2013.01.037

Cited by 73 publications

(54 citation statements)

References 27 publications

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“…For this purpose, it is required to find out the most orthogonal conditions and the parameters which have the highest impact on retention and selectivity when using HILIC conditions. It has been demonstrated that the mobile phase pH and nature of the stationary phase were the most important variables in HILIC [206,207]. Regarding pH, two initial conditions are sufficient for the screening procedure, namely pH 3 and 6.…”

Section: Current Trends In Hilicmentioning

confidence: 99%

“…However, such pH conditions are only compatible with a restricted number of stationary phases such as hybrid silica, and cannot be easily employed on any HILIC phases. In terms of stationary phases, a lot of studies have been recently published to find out the most different HILIC columns [206,[208][209][210][211][212]. Based on multivariate data analysis and using large set of diverse compounds, it appears that a limited number of stationary phases, namely 4 to 5 different chemistries are sufficient for the initial screening procedure.…”

Section: Current Trends In Hilicmentioning

confidence: 99%

“…Among them, the most promising ones are: (i) unmodified bare or hybrid silica, despite a possible strong adsorption of various types of polar compounds, (ii) amide bonded phase, because of its high versatility for high performance separation of polar drugs, (iii) amino phase thanks to the sufficient retention of negatively charged compounds such as acids, and (iv) zwitterionic sulfoalkylbetaine or poly(succinimide) bonded phases which also offer suitable retention for a wide range of analytes in spite of a lower kinetic performance compared to the other supports. As example, Periat et al [206] proposed a screening strategy involving exclusively short columns (50 x 2.1 mm) packed with sub-2 m particles, including unmodified bare silica, bare hybrid silica, silica bonded with zwitterionic group and hybrid silica bonded with amide. When testing these four columns at pH 3 and 6, and a generic gradient from 95 to 65% v/v ACN, all the screening procedure was performed in less than 1 hour, reequilibration included.…”

Section: Current Trends In Hilicmentioning

confidence: 99%

See 2 more Smart Citations

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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Section: Current Trends In Hilicmentioning

confidence: 99%

Section: Current Trends In Hilicmentioning

confidence: 99%

Section: Current Trends In Hilicmentioning

confidence: 99%

See 1 more Smart Citation

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

Self Cite

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“…However, the choice of organic modifier, column temperature and ionic strength of buffer appear to have less impact on these important variables 3 .…”

Section: Hydrophilicmentioning

confidence: 99%

“…Interaction Chromatography (HILIC): To fully utilise the low back pressures and decreased viscosities generated using the HILIC operating mode, it is advantageous to develop HILIC methods using <2µm stationary phases 3 . This can be done using classical HPLC hardware, rather than any requirement to use UPLC equipment-provided that the extra column volumes and dwell volumes are aligned with the column dimensions 43,44 .…”

Section: Hydrophilicmentioning

confidence: 99%

Untitled

2017

IJPSR

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Impurities will be present in all drug substances and drug products, i.e. nothing is 100% pure if one looks in enough depth. The current regulatory guidance on impurities accepts this, and for drug products with a dose of less than 2 g/day identification of impurities is set at 0.1% levels and above (ICH Q3B (R2), 2006). For some impurities, this is a simple undertaking as generally available analytical techniques can address the prevailing analytical challenges; whereas, for others this may be much more challenging requiring more sophisticated analytical approaches. The present review provides an insight into current development of analytical techniques to investigate and quantify impurities in drug substances and drug products providing discussion of progress particular within the field of chromatography to ensure separation of and quantification of those related impurities. Further, a section is devoted to the identification of classical impurities, but in addition, inorganic (metal residues) and solid state impurities are also discussed. Risk control strategies for pharmaceutical impurities aligned with several of the ICH guidelines, are also discussed.

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Hydrophilic Interaction Liquid Chromatography of Small Molecules

Gama

Bottoli

2018

Encyclopedia of Analytical Chemistry

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Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to separation of mixtures of polar and hydrophilic compounds that are poorly resolved by reversed‐phase liquid chromatography. This separation mode involves polar stationary phases and an aqueous mobile phase, usually rich in an organic solvent such as acetonitrile (ACN). In addition to the commonly accepted mechanism of analyte liquid–liquid partitioning between the mobile phase and the water‐enriched solvent layer, which is partially immobilized onto the surface of the stationary phase, hydrogen bonding, hydrophobic interaction, and ion‐exchange interactions may also be involved. HILIC applications for the analysis of small polar molecules can be found in pharmaceutical, medicinal, food, and agricultural chemistry. Carbohydrates, amino acids, nucleotides, polar drugs, metabolites, toxins, and other small polar or ionizable compounds are typical HILIC analytes.

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Screening of the most relevant parameters for method development in ultra-high performance hydrophilic interaction chromatography

Cited by 73 publications

References 27 publications

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Untitled

Hydrophilic Interaction Liquid Chromatography of Small Molecules

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