Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents

Abstract

The antimicrobial properties of herbs from Papaveraceae have been used in medicine for centuries. Nevertheless, mutual relationships between the individual bioactive substances contained in these plants remain poorly elucidated. In this work, phytochemical composition of extracts from the aerial and underground parts of five Papaveraceae species (Chelidonium majus L., Corydalis cava (L.) Schweigg. and Körte, C. cheilanthifolia Hemsl., C. pumila (Host) Rchb., and Fumaria vaillantii Loisel.) were examined using LC-ESI-MS/MS with a triple quadrupole analyzer. Large differences in the quality and quantity of all analyzed compounds were observed between species of different genera and also within one genus. Two groups of metabolites predominated in the phytochemical profiles. These were isoquinoline alkaloids and, in smaller amounts, non-phenolic carboxylic acids and phenolic compounds. In aerial and underground parts, 22 and 20 compounds were detected, respectively. These included: seven isoquinoline alkaloids: protopine, allocryptopine, coptisine, berberine, chelidonine, sanguinarine, and chelerythrine; five of their derivatives as well as non-alkaloids: malic acid, trans-aconitic acid, quinic acid, salicylic acid, trans-caffeic acid, p-coumaric acid, chlorogenic acid, quercetin, and kaempferol; and vanillin. The aerial parts were much richer in phenolic compounds regardless of the plant species. Characterized extracts were studied for their antimicrobial potential against planktonic and biofilm-producing cells of S. aureus, P. aeruginosa, and C. albicans. The impact of the extracts on cellular metabolic activity and biofilm biomass production was evaluated. Moreover, the antimicrobial activity of the extracts introduced to the polymeric carrier made of bacterial cellulose was assessed. Extracts of C. cheilanthifolia were found to be the most effective against all tested human pathogens. Multiple regression tests indicated a high antimicrobial impact of quercetin in extracts of aerial parts against planktonic cells of S. aureus, P. aeruginosa, and C. albicans, and no direct correlation between the composition of other bioactive substances and the results of antimicrobial activity were found. Conclusively, further investigations are required to identify the relations between recognized and unrecognized compounds within extracts and their biological properties.

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

“…Finally, the cytotoxicity against stomach-and liver-derived cells may be associated with an inflammatory response manifested by cytokine expression and release. In our previous study on lipopolysaccharide-stimulated human neutrophils [57], we found that several alkaloids (sanguinarine, berberine, chelidonine, and chelerythrine) contained in the extracts from both tested plants [56], depending on the specific concentrations, had a dual anti-inflammatory and pro-inflammatory effect. Hence, the inflammatory response of stomach and liver cells and tissues upon treatment with the antibacterial extracts should be considered in future studies, including in vivo models.…”

“…However, as this potentially undesired effect was not actually studied, it opens an opportunity for further investigation into the mechanisms by which the pathogenic bacteria may cope with such combined therapeutics. Despite many reports on differences between microbial strains in sensitivity to plant extracts, no data were found in the literature on specific mechanisms that would confer the resistance against complex mixtures of natural products containing both alkaloids and polyphenols [56]. Certainly, this issue needs to be addressed in future research aiming at verification of whether H. pylori is able to develop resistance to these and other plant-derived antibacterials and what would be the potential mechanisms of it.…”

Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from Chelidonium majus Plant Cell Cultures

“…The advanced technology created features of BNC that enable to use it in medicine and pharmaceutical industry as biotechnology-based products or biopharmaceutics. Our proof of concept based on the biotechnological process of cells immobilization, both plant and bacterial, on the cellulose carrier let to expose the antimicrobial and anti-inflammatory properties of plant metabolites even stronger than when used separately [ 34 ]. The carrier ( Figure 1 ) showed differences in physical properties ( Table 1 ) correlated with the biosynthesis time (3, 5 or 7 days).…”

“…Our earlier studies have shown that C. majus cell cultures are rich in polyphenolic compounds and isoquinoline alkaloids with confirmed antimicrobial and anti-inflammatory properties [ 8 , 10 , 11 ]. The major metabolites secreted by BNC-immobilized C. majus cells were chlorogenic acid, trans-caffeic acid and quercetin; all these compounds are known for their antimicrobial activity and all of them were found in the aerial parts of naturally growing C. majus [ 34 ]. One of our main concerns, with regard to the applicability of our work, was potential inflammatory effect which could be displayed by NC-type BNC carriers, containing live K. xylinus cells.…”

Plant Natural Products as Antimicrobials for Control of Streptomyces scabies: A Causative Agent of the Common Scab Disease

“…For example, quercetin has strong antioxidant, antibacterial and antiparasite properties (Yang et al, 2020); it also binds specifically to GyrB, a subunit of Escherichia coli DNA gyrase, inhibiting its ATPase activity (Plaper et al, 2003). Quercetin also has strong antimicrobial potential against planktonic bacteria as well as disrupting biofilm formation of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans (Zielinska et al, 2021).…”