Screening Procedures for Chloramphenicol Residue Determination in Serum, Milk, and Feedstuff Using Immunochromatographic Assay

Hsieh, Kuan Huei; Lin, Chiao-Po; Cheng, Chu; Lai, Choon-Sheen; Sheu, Shi Yuan; Yao, Chun‐Hsu; Lei, Yi; Chang, Tong; Kuo, Ting‐Shen

doi:10.1080/08905436.2013.838784

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…Compared with chromatographic methods, immunoassays are simple, rapid and do not require expensive equipment and professional operator. In conclusion, the LFA has proven to be a suitable tool for onsite test to detect various types of analytes (Hsieh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang

Zheng

et al. 2017

Food and Agricultural Immunology

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A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L −1 , respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC-MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang

Zheng

et al. 2017

Food and Agricultural Immunology

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“…It can be observed that the ELISA method overestimated the low values (minimum detected level was 0.6 µg kg –1 ) giving rise to a number of ‘false-positives’ compared with LC-MS/MS where samples apparently free from CAP were present (LOD = 0.05 µg kg –1 ). This might be explained by cross-reactivity of trace amounts of structurally similar compounds, as is also sometimes observed when applying this test in animal feed testing (Hsieh et al 2013 ). Of the ‘validation’ set consisting of 26 samples, five were erroneously classified as having a CAP content of more than 1 µg kg –1 by ELISA analysis.…”

Section: Resultsmentioning

confidence: 76%

Occurrence of chloramphenicol in cereal straw in north-western Europe

Nordkvist

Zuidema

Herbes

et al. 2016

Food Additives & Contaminants: Part A

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Two surveys are presented of straw analysed for naturally occurring chloramphenicol (CAP), a drug banned for use in food-producing animals. In the first study, CAP was analysed by LC-MS/MS and detected in 37 out of 105 straw samples originating from the Netherlands, France, the UK, Germany and Denmark. The highest level found was 6.3 µg kg−1, the average 0.6 µg kg−1 and the median 0.2 µg kg−1. The second study included a method comparison between ELISA and LC-MS/MS and a survey of CAP in cereal straw sampled at farms in all areas of Sweden. A total of 215 samples were screened by ELISA and a subset of 26 samples was also analysed by LC-MS/MS. Fifty-four of the samples contained more than 1 µg kg−1 CAP and the highest level found was 32 µg kg−1 (confirmed by LC-MS/MS). The highest contents of CAP in this study were allocated to the Baltic sea coast in the south-eastern part of Sweden (the county of Skåne and the Baltic Sea isle of Gotland). These results indicate a high incidence of CAP in straw in north-west Europe and have a severe impact on the enforcement of European Union legislation.

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“…In recent years, immunoaffinity chromatography cleanup has been used for sample pretreatment in instrument analyses, which can save organic solvents and improve efficiency (Luo et al, 2010;Zhang et al, 2013a;Wang et al, 2014). Based on these antibodies, some immunochromatographic assays have also been exploited for the rapid detection of fenicol residues (Byzova et al, 2010;Berlina et al, 2013;Hsieh et al, 2013;Zhu et al, 2014). In contrast to chromatographic methods, immunological methods are simple, rapid and do not require professional training for the operator.…”

Section: Introductionmentioning

confidence: 99%

Comparsion of an immunochromatographic strip with ELISA for simultaneous detection of thiamphenicol, florfenicol and chloramphenicol in food samples

Guo

Song

Liu

et al. 2015

Biomedical Chromatography

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Rapid and sensitive indirect competitive enzyme-linked immunosorbent assays (ic-ELISA) and gold nanoparticle immunochromatographic strip tests were developed to detect thiamphenicol (TAP), florfenicol (FF) and chloramphenicol (CAP) in milk and honey samples. The generic monoclonal antibody for TAP, FF and CAP was prepared based on a hapten [D-threo-1-(4-aminophenyl)-2- dichloroacetylamino-1,3-propanediol], and the haptenwas linked to a carrier protein using the diazotization method. After the optimization of several parameters (coating, pH, sodium chloride content and methanol content), the ic-ELISA was established. The quantitative working range for TAP was 0.11-1.36 ng/mL, with an IC50 of 0.39 ng/mL. The optimized ELISA showed cross-reactivity to CAP (300%) and FF (15.6%), with IC50 values of 0.13 and 2.5 ng/mL, respectively. The analytical recovery of TAP, FF and CAP in milk and honey samples in the ic-ELISA ranged from 81.2 to 112.9%. Based on this monoclonal antibody, a rapid and sensitive immunochromatographic test strip was also developed. This strip had a detection limit of 1 ng/mL for TAP, FF and CAP in milk and honey samples. Moreover, the test was completed within 10 min. Our results showed that the proposed ic-ELISA and immunochromatographic test strip method are highly useful screening tools for TAP, FF and CAP detection in milk and honey samples.

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Screening Procedures for Chloramphenicol Residue Determination in Serum, Milk, and Feedstuff Using Immunochromatographic Assay

Cited by 8 publications

References 13 publications

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Occurrence of chloramphenicol in cereal straw in north-western Europe

Comparsion of an immunochromatographic strip with ELISA for simultaneous detection of thiamphenicol, florfenicol and chloramphenicol in food samples

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