Screening, recognition and quantitation of salbutamol residues in ham sausages by molecularly imprinted solid phase extraction coupled with high-performance liquid chromatography–ultraviolet detection

Yan, Hongyuan; Wang, Ruiling; Han, Yehong; Liu, Suting

doi:10.1016/j.jchromb.2012.05.021

Cited by 31 publications

(14 citation statements)

References 39 publications

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“…A number of sample pre‐treatment methods, based on novel adsorbents, have been developed for the analysis of SAL. C 18 ‐SPE (Fiori et al ., ), weak cation exchange columns (Meng et al ., ), molecularly imprinted polymers (Yan et al ., ), polymer microextraction (Huang et al ., ; Du et al ., ), immunoaffinity columns (Wang et al ., ), tert ‐butylmethylether extraction (Garrido et al ., 2013) and poly (MAA‐EGDMA) SPE columns (Wang et al ., ) have been used to extract SAL from the complex biological samples. However, most of these methods are nonspecific and may lead to nonspecific adsorption from the sample matrix.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

Fan

Wang

Wei

2015

Biomedical Chromatography

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Salbutamol (SAL) is the most widely used β2 -agonist drug for asthma and chronic obstructive pulmonary patients, but it is also often abused as feed additive. In recent years, the abuse of SAL has led to a large number of food safety incidents. Therefore, the monitoring of SAL residues in animal products is very important. A highly selective boronate affinity magnetic adsorbent was synthesized and developed for detection of trace levels of SAL residues in pig tissue samples. The obtained Fe3O4@SiO2@FPBA(4-formylphenylboronic acid) magnetic adsorbent showed good adsorption ability to catechol and SAL, and then it was successfully applied as special magnetic solid-phase phase extraction adsorbent coupled with high-performance liquid chromatography (HPLC) for simultaneous isolation and determination of cis-diol compounds. The binding capacity of catechol and SAL reached 96 and 50 µmol/g, respectively. The method was successfully established for the detection of trace levels of SAL in pig tissue samples. The linear range extended from 0.32 to 800 µg/kg (R(2) = 0.9994). The limit of detection of SAL was 0.19 µg/kg. The recoveries were satisfactory (89.5-108.0%) at three spiked levels with RSD between 2.1 and 11.3%. These results indicated that the method has potential for enrichment and detection of trace levels of SAL residual in animal food products.

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Section: Introductionmentioning

confidence: 99%

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

Fan

Wang

Wei

2015

Biomedical Chromatography

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“…Several analytical methods for the determination of salbutamol were developed and reported such as TLC (Dave et al, 2011), GC and GC-MS (Caban et al, 2011;Wang et al, 2010), cyclic voltammetry (Ganjali et al, 2005, adsorptive stripping voltammetry on a carbon paste electrode (Attaran et al, 2012), Capillary electrophoresis coupled with electrochemilumencesis (Bao et al, 2012), flow injection method (Al-Abachi and Subhi, 2013), HPLC and RP-HPLC (Mukesh and Ranjit, 2011;Pai et al, 2009 ;Ghulam et al, 2009 ;Li et al, 2010), HPLC-chemiluminescence (Zhang et al, 2011), solid phase extraction (SPE)-HPLC (Liu and Wang , 2011;Yan et al, 2012) , and fluorescence (Tang et al, 2010).…”

Section: Salbutamol Sulphatementioning

confidence: 99%

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

Othman¹,

Asaad²

2014

RJS

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A simple, rapid and sensitive spectrophotometic method for the estimation of trace amounts of salbutamol sulphate (SBS) in pharmaceutical preparations has been proposed .The method is based on the coupling reaction of the intended compound with diazotized 5-amino-2chlorobenzotrifluoride in alkaline medium and in the presence of Triton X-100 to form a yelloworange dye that shows maximum absorption at 467 nm. Beer's law is obeyed over the range 5-300 µg / 10 ml (i.e.,0.5-30 ppm) with a molar absorptivity of 4.20 × 10 4 l.mol-1 .cm-1 and Sandell's sensitivity index of 0.0139 µg.cm-2 , a relative error of +1.08 to +3.46 % and a relative standard deviation of ±0.76 to ±3.50 %, depending on the concentration. The method has been applied to estimate salbutamol sulphate in syrup, tablet and ventolin.

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“…To date, the reported methods for detecting SAL in breeding farms and meat-processing enterprises were mainly chromatographic methods, including liquid chromatographytandem mass spectrometry (LC-MS/MS) (González-Antuna et al 2014), high performance liquid chromatography (HPLC) (Yan et al 2012), gas chromatography-mass spectrometry (GC-MS) (Caban et al 2011). Although these chromatographic methods can provide sensitive detection and accurate identification, the drawbacks of expensive instruments, complex pretreatment process, and time-consuming, professional operation, etc.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive and Specific Detection of Salbutamol in Swine Feed, Meat, and Urine Samples by a Competitive Immunochromatographic Test Integrated with Surface-Enhanced Raman Scattering

et al. 2016

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Salbutamol (SAL), a kind of β-agonist which can enhance the lean meat-to-fat ratio, has been inhibited as an additive used in animal feeds for livestock production in many countries due to its harmful effect to the consumers. In this study, an ultrasensitive and specific competitive immunochromatographic test (ICT) integrated with surfaceenhanced Raman scattering (SERS) for the detection of SAL was described. The immunoprobe was prepared by immobilizing polyclonal antibody against SAL on the surface of Au@Ag nanoparticles in which the Raman reporter (4-mercaptobenzoic acid, MBA) had been sandwiched. After ICT procedures, the specific SERS signals generated from MBA on the test line of the ICT strip were measured for the quantitative determination of SAL. The assay was completed in 15 min. The IC 50 and the limit of detection (LOD) values of the assay for SAL were 0.028 ng mL −1 and 3.0 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with other three β-agonists (clenbuterol, phenylethanolamine A, and ractopamine), showing high specificity of the assay. Spiking experiments indicated that the average recoveries (n = 3) of SAL from swine feed, meat, and urine samples were in ranges of 98.4-105.2 % with the relative standard deviations (RSDs) of 1.7-7.8 %. The results demonstrated that the proposed ICT was a feasible method for ultrasensitive and specific detection SAL in swine feed, meat and urine samples, and could be extended for the detection of other target analytes.

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Screening, recognition and quantitation of salbutamol residues in ham sausages by molecularly imprinted solid phase extraction coupled with high-performance liquid chromatography–ultraviolet detection

Cited by 31 publications

References 39 publications

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

Synthesis and application of boronic acid‐functionalized magnetic adsorbent for sensitive analysis of salbutamol residues in pig tissues

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

Ultrasensitive and Specific Detection of Salbutamol in Swine Feed, Meat, and Urine Samples by a Competitive Immunochromatographic Test Integrated with Surface-Enhanced Raman Scattering

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