This study aimed to evaluate the influence of a post-machining thermal treatment on canal-centering ability and torque/force generation of reciprocating nickel-titanium instruments. Simulated J-shaped resin canals were prepared with reciprocating instruments sharing identical geometric architecture and with/without post-machining thermal treatment (Reciproc Blue/Reciproc, VDW, Munich, Germany). Using an original automated root canal instrumentation and torque/force analyzing device, files were operated in a combination of reciprocation and up-and-down motion, and torque/force values were monitored. Canal-centering ratios were measured after superimposition of pre-and post-instrumentation images. Compared with Reciproc, Reciproc Blue showed a significantly lower canal-centering ratio (i.e., less deviation; p<0.05) at 0-1 mm from the apex and generated a significantly smaller upward maximum vertical force (p<0.05). Under standardized conditions using the automated device, Reciproc Blue showed better canal-centering ability and reduced screw-in forces than Reciproc, indicating that the post-machining thermal treatment confers superior performance to reciprocating nickel-titanium instruments.