Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Руденко, П. А.; Vatnikov, Y.A.; Sachivkina, Nadezhda; Rudenko, Andrei; Kulikov, E.V.; Lutsay, Vladimir; Notina, Elena; Bykova, Irina; Petrov, A.Ya.; Drukovsky, Stanislav Gennadievich; Olabode, Ifarajimi Rapheal

doi:10.3390/pathogens10060667

Cited by 31 publications

(38 citation statements)

References 43 publications

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“…Infectious diseases can produce complications due to the formation of microbial biofilms in the body. Many chronic fungal and bacterial diseases are associated with biofilms, since the biofilms adhere strongly to the cells of the human or animal body, and are less susceptible to antibiotic therapy than individual cells [ 16 - 21 ].…”

Section: Discussionmentioning

confidence: 99%

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Sachivkina

Podoprigora

2021

Vet World

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Background and Aim: Different Candida species isolated in humans and animals have different types of parasite activity. The most pathogenic species is Candida albicans followed by Candida tropicalis. However, the effects of the morphology of Candida krusei, Candida guilliermondii, and Candida glabrata biofilms on the pathogenicity of these species have not been fully characterized. To the best of our knowledge, there is no literature on the effect of farnesol on rare Candida species. This study aimed to check the effect of different farnesol concentrations on the species C. krusei, C. guilliermondii, and C. glabrata compared with the strain C. albicans ATCC 10231, which has been widely studied, and is a strong producer of biofilms. Materials and Methods: We studied the morphological and densitometric parameters of biofilms produced by Candida species under the influence of the drug farnesol (Sigma-Aldrich, St. Louis, MO). We used a heart brain broth with the addition of 2% bovine blood serum in 96-well plates. To each well, we added 100 μL of C. albicans, C. krusei, C. guilliermondii, or C. glabrata culture, and 0.2-400 μM farnesol. The microliter plates were cultured with the lid closed at 37°C for 48 h. Then, the liquid was removed, and the wells were washed 3 times with 200 μL phosphate buffer solution (pH 7.3). Biofilm fixation was performed using 150 μL of 96% ethanol for 15 min. Then, the microliter plates were dried for 20 min at 37°C, a 0.5% solution of crystalline violet was added, and the plates were placed in an incubator at 37°C. After 5 min, the contents of the wells were removed, washed 3 times with 200 μL of phosphate buffer solution (pH 7.2), and dried. The dye was extracted by washing with 200 μL of 96% ethanol for 30 min. The results were obtained using a photometric analyzer of enzyme immunoassay reactions at an optical density (OD) wavelength of 450 nm. Results: All of Candida spp. strains tested were susceptible to farnesol at concentrations ranging from 0.8 to 400 μM for C. albicans, C. krusei, and C. guilliermondii, and 12.5 to 400 μM for C. glabrata. Conclusion: This study provides new insights into the use of farnesol against biofilms produced by Candida species, but further studies in vivo are necessary to evaluate the effectiveness of the reduction of OD. To the best of our knowledge, the antimicrobial activity of farnesol against C. krusei, C. guilliermondii, and C. glabrata has not been reported previously, although studies have confirmed the inhibitory effect of farnesol on the growth of different microorganisms.

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Section: Discussionmentioning

confidence: 99%

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Sachivkina

Podoprigora

2021

Vet World

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“…A number of recent studies have revealed the features of the intestinal microbiome in cats in normal conditions, as well as in various pathological conditions [11,14,18,33]. However, these studies did not analyze the fecal gut microbiota in cats with dysbiosis of varying severity.…”

Section: Discussionmentioning

confidence: 99%

“…A number of undeniable advantages are inherent in small animals: they improve our mood, reduce emotional and physical stress, help their owners cope with depression, create a feeling of exaltation, as well as a warm and cozy atmosphere in the hearth. In addition, dogs and cats contribute to the psychological development of children with autism, cerebral palsy, and developmental delays and improve the quality of life for lonely, elderly people and patients with cardiovascular pathologies [8][9][10][11]. However, despite the rapid development of veterinary medicine, many issues of diseases in small domestic animals, which are caused by associations of opportunistic and saprophytic microbiota, as well as the improvement of diagnostic approaches, prevention, and therapy, remain poorly understood [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…The relationship between microbiota and their host does not take place in isolation with each individual but associatively as part of the microbiocenosis, which is an open, unstable, constantly changing group of evolutionarily ecologically related microorganisms [15,[17][18][19]. Under the influence of environmental factors, unsatisfactory nutrition, systematic violations of elementary veterinary and sanitary rules of keeping, failure to fully implement measures to prevent infectious diseases, and irrational antibiotic therapy, the body's immune system may suffer, and as a result, various immunodeficiency states arise in the animal [11,13,20]. As a result, not only conditionally pathogenic microflora are activated but even saprophytic microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…The study of the intestinal microbiota's role has convincingly shown that they are the most important component of the protective intestinal barrier, which controls the interaction of the macroorganism and its environment [11,[24][25][26]. The gastrointestinal microbiome is a multicomponent consortium of bacteria, archaea, fungi, protozoa, and viruses that inhabit the intestines of all mammals.…”

Section: Introductionmentioning

confidence: 99%

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Fecal Microbiota Analysis in Cats with Intestinal Dysbiosis of Varying Severity

et al. 2022

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Recent studies have shown that the gut microbiota plays an important role in the pathogenesis of gastrointestinal diseases in various animal species. There are only limited data on the microbiome in cats with varying grades of dysbiosis. The purpose of the study was a detailed analysis of the quantitative and qualitative fecal microbiota spectrum in cats with intestinal dysbiosis of varying severity. The data obtained indicate that, depending on the dysbiosis severity in cats, the intestinal microbiome landscape changes significantly. It has been established that, depending on the dysbiosis severity, there is a shift in the balance between the Gram-positive and Gram-negative bacterial pools and in the nature of the isolation of specific bacteria forms, in the amount of obligate microbiota isolation, as well as individual facultative strains. When analyzing the serotyping of E. coli cultures isolated at various grades of intestinal dysbiosis severity, differences were found both in the isolation amount of various serotypes from one animal and in the prevalence of certain serotypes for each disease severity. A retrospective analysis of the fecal microbiota sensitivity in cats with dysbiosis to antibacterial drugs showed that, depending on the disease severity, the number of isolates sensitive to antibiotics increases significantly.

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Influence of Protozoosis of Small Intestine Cystoisospora Sp. And Giardia Sp. On Hematological Indicators in Dogs

Lykhina

Кузнецов

Telezhenkova

et al. 2023

Lecture Notes in Networks and Systems

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Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Cited by 31 publications

References 43 publications

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Fecal Microbiota Analysis in Cats with Intestinal Dysbiosis of Varying Severity

Influence of Protozoosis of Small Intestine Cystoisospora Sp. And Giardia Sp. On Hematological Indicators in Dogs

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