2008
DOI: 10.1111/j.1365-2818.2008.02086.x
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Second‐harmonic microscopy of ex vivo porcine corneas

Abstract: SummaryThe ex vivo cornea of porcine eyes has been studied with second-harmonic microscopy with a laboratory-built system to examine the structure of collagen fibrils at different length scales, as well as the image dependence on polarization and wavelength of the illumination source. We found that collagen fibrils can effectively be visualized with secondharmonic microscopy, in agreement with previous findings, at different wavelengths of the illumination. The same laser source used for imaging may also be us… Show more

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Cited by 11 publications
(9 citation statements)
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“…This imaging modality has already been demonstrated to be able to evaluate the structural organization of the porcine cornea stroma. 50,51 As seen in Fig. 6, forward and backward detected SHG produce fairly distinct images [Figs.…”
Section: Discussionmentioning
confidence: 97%
“…This imaging modality has already been demonstrated to be able to evaluate the structural organization of the porcine cornea stroma. 50,51 As seen in Fig. 6, forward and backward detected SHG produce fairly distinct images [Figs.…”
Section: Discussionmentioning
confidence: 97%
“…Structural alterations of the cornea because of scars have been detected using multiphoton microscopy. 14 The effect of polarization and wavelength 15,16 on the forward and backward SHG signals from porcine corneas have also been evaluated. Moreover, the combination of SHG and third harmonic generation signal has been applied to resolve the porcine corneal layers.…”
mentioning
confidence: 99%
“…Als kritisch muss die noch lückenhafte Erklärung für die wesentlichen Unterschiede zwischen Vorwärts-und Rückwärts-SHG angesehen werden. Zusätzlich könnte hier eine gleichzeitige Beobachtung der Transparenz in Durchstrahlrichtung, wie sie von Vohnsen et al [31] durch Detektion der Grundwelle durchgeführt wurde, für eine bessere Abgrenzung zwischen an getrübter Hornhaut zurückgestreutem ursprünglichem Vorwärts-SHG-Signal und direkt an der Struktur rückwärtig erzeugtem SHG-Licht zu unterscheiden. Für die anatomische Interpretation der Strukturen wäre es ideal, wenn man von der Streifung im Vorwärtssignal mathematisch auf die genaue Stellung der Kollagen Fibrillen, ihre Dicke und Abstände schließen könnte.…”
Section: Methodische Kritik Und Verbesserungsmöglichkeitenunclassified