Secondary oxidants in human serum exposed to singlet oxygen: the influence of hemolysis

David, Olivier; Douillard, Samuel; Lhommeau, Isabelle; Bigot, Edith; Patrice, Thierry

doi:10.1039/b9pp00032a

Cited by 18 publications

(13 citation statements)

References 47 publications

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“…Profiles of DCF fluorescence evolution as well as the AUC values were similar to previous data [21]. From preliminary data, we noted that the evolution of DCF fluorescence after photosensitization of one given human serum vial by RB under similar conditions was found to be highly reproducible [21].…”

Section: Resultssupporting

confidence: 83%

“…From preliminary data, we noted that the evolution of DCF fluorescence after photosensitization of one given human serum vial by RB under similar conditions was found to be highly reproducible [21]. In the present study, the standard deviation (SD) was always less than 0.05 and rarely above 0.03 for a given compound added to a given serum.…”

Section: Resultssupporting

confidence: 54%

“…The principle of the measurement [21] is to produce a standardized amount of photodynamically-induced 1 O 2 using Rose Bengal (RB, (CAS N° 632-69-9), 5 µg/mL final concentration) as a source of 1 O 2 under light exposure (514 nm, 500 mW, 20 J/cm 2 ) in the serum to be assayed (25 µL in 450 µL water for injections) and to measure the speed of neutralization of secondary ROS and/or peroxides. Light was delivered from an argon ion laser by means of an optic fibre (400 µm core diameter).…”

Section: Methodsmentioning

confidence: 99%

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Influence of Vitamins on Secondary Reactive Oxygen Species Production in Sera of Patients with Resectable NSCLC

et al. 2016

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Background: Singlet oxygen (1O2) oxidizes targets through the production of secondary reactive oxygen species (SOS). Cancers induce oxidative stress changing with progression, the resulting antioxidant status differing from one patient to the other. The aim of this study was to determine the oxidative status of patients with resectable Non-Small cell lung cancers (NSCLC) and the potential influence of antioxidants, compared to sera from healthy donors. Materials and Methods: Serum samples from 10 women and 28 men, 19 adenocarcinomas (ADK), 15 patients N1 or M1 were submitted to a photoreaction producing 1O2. Then, samples were supplemented with vitamins (Vit C, Vit E), or glutathione (GSH). Results: Squamous cell carcinomas (SCC) and metastatic SCCs induced a lower SOS rate. While Vit C increased SOS in controls as in patients with metastases, Vit E or the combination of Vit E and C strongly reduced SOS. GSH alone lightly decreased SOS in controls but had no effect in patients either alone or combined with Vit C. Conclusion: In “early” lung cancers, SOS are comparable or lower than for healthy persons. The role of Vitamins varies with gender, cancer type, and metastases. This suggests that an eventual supplementation should be performed on a per-patient basis to evidence any effect.

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Section: Resultssupporting

confidence: 83%

Section: Resultssupporting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

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Influence of Vitamins on Secondary Reactive Oxygen Species Production in Sera of Patients with Resectable NSCLC

et al. 2016

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“…The production of O requires oxygen, but its overall effect also depends on the way it is deactivated into SOS. In addition, the very limited radius of action of O , (Moan and Berg 1991;Snyder and Skovsen et al 2006), due to its short lifespan, makes additional mechanistic pathways plausible to explain all described effects, increasing the sole O effects (Chakraborty and Held et al 2009;Olivier and Douillard et al 2009a). …”

Section: Discussionmentioning

confidence: 97%

Influence of Animal Species on Secondary Oxidant Activation in Serum Exposed to Singlet Oxygen

Lhommeau¹,

Douillard²,

Foursac³

et al. 2012

ILAR Journal

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Singlet oxygen ((1)O(2)) produced during inflammatory reactions and during photodynamic therapy deactivates by producing in tissues secondary reactive oxygen species and peroxides (SOS) as well as other degradation products. We investigated the influence of animal species on SOS production secondary to standardized (1)O(2) production by performing in vitro experiments with rose bengal as the (1)O(2) producer, human serum (HS) as a control, sera derived from various animal species, and dichloro-dihydro-fluorescein as a nonspecific marker that becomes fluorescent when oxidized. The overall SOS production in HS from a presumed healthy cohort of 53 donors (31 males and 22 females) gave a mean "normal" value of 0.91 compared with a previous pool of 75 male sera samples. SOS production after a photo-reaction was two or four times lower in HS than in fetal calf serum or mice sera, respectively. In mice, the "nude" characteristic increased even more than in the SOS production. In the Aves order, this production appeared to be distributed randomly according to the number of branches after the appearance of Amniotas. For primates, SOS production appeared to decrease linearly with the number of branches (R(2) = 0.98). Adding hemolysates from complete bloods to the corresponding sera induced an increase in SOS production in all species, proportional to the production in sera. These findings should be kept in mind when interpreting results from studies of secondary reactive oxygen species-induced pathways following (1)O(2) production, regardless of its origin.

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“…Still an emerging body of information points to singlet oxygen being a significant intracellular effector and regulator. In addition, there are indications that the deactivation of secondary oxidants in Human serum resulting from exposure to 1 O 2 is affected by the serum type and number of platelets [77].…”

Section: Platelet Regulatory Mediators In Pdtmentioning

confidence: 99%

Platelets, photosensitizers, and PDT

Senge

Radomski

2013

Photodiagnosis and Photodynamic Therapy

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SummaryPhotodynamic therapy (PDT) has both direct cell and indirect vascular effects. Both are well established and the latter has given rise to specifically target angiogenesis in PDT treatments. While the vascular effects are well understood, further advances in antiangiogenesis require a detailed understanding of PDT effects at the microvasculature level. Although some of the earliest investigations of PDT noted effects on platelet aggregation the importance of their interaction with endothelial cells and the crucial role of various signaling pathways is only now emerging. This review aims to give a general overview of PDT related studies on platelets, vascular effects, and their interaction with endothelial cells to indicate the potential for studies in this area.Abbreviations: ALA --aminolevulinic acid; AMD -age-related macular degeneration; ANET -anti-neovascular therapy; BGF -basic fibroblast growth factor; DHEdihaematoporphyrin ether; EDRF -endothelium-derived relaxing factor; epithelial growth factor -EGF; HIF-1 -hypoxia-inducible factor 1; HpD -haematoporphyrin derivative; IL -interleukin; PAF -platelet activating factor; PDT -photodynamic therapy; PS -photosensitizer; transforming growth factor 1 -TGF-1; TNF -tumor necrosis factor.

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Secondary oxidants in human serum exposed to singlet oxygen: the influence of hemolysis

Cited by 18 publications

References 47 publications

Influence of Vitamins on Secondary Reactive Oxygen Species Production in Sera of Patients with Resectable NSCLC

Influence of Vitamins on Secondary Reactive Oxygen Species Production in Sera of Patients with Resectable NSCLC

Influence of Animal Species on Secondary Oxidant Activation in Serum Exposed to Singlet Oxygen

Platelets, photosensitizers, and PDT

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