Secretion of Activin by Interstitial Cells in the Testis

Lee, Will; Mason, Anthony J.; Schwall, Ralph; Szonyi, Eva; Mather, Jennie P.

doi:10.1126/science.2492117

Cited by 98 publications

(31 citation statements)

References 19 publications

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“…These secretory granules are also associated with a specific secretory mechanism called the regulatory pathway (55). It is now clear that peptide hormones may be secreted by steroid producing cells (50), of which the well known peptide secretions of the placenta is the best example (56 (27). It Our results show that in a tissue which normally expresses the POMC gene predominantly as a short, truncated RNA, activation of a pituitary promoter may induce a dramatic rise in the production of POMC peptides.…”

Section: Resultsmentioning

confidence: 99%

“…Altered modes of POMC processing have been described in human ACTH-producing nonpituitary tumors, where it can either be absent (41,45) Recently, various peptide hormones, and in some cases their mRNA, have been identified in cell types which were thought (up to now) to produce only steroid hormones; inhibin in the granulosa cells (46) and prorenin in the theca cells of the human ovary (47) are two examples. In the Leydig cell itself, renin (48), enkephalin (49), activin (50), and AVP (51) are other recent examples. The presence of AVP, as fully characterized by HPLC, is a further demonstration that polypeptide precursor processing can occur in the testis, also stressing the fact that processing enzymes may have a wide tissue distribution, although they are not present in all cell types (52).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors.

Keyzer

Lenne²,

Massias³

et al. 1990

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors.

Keyzer

Lenne²,

Massias³

et al. 1990

J. Clin. Invest.

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“…It also enhanced LH-mediated androgen production in Leydig cells [11,15]. Although it has been generally accepted that inhibin is produced in Sertoli cells [2,6,20,24,26] and activin in Leydig cells [14], there was a recent evidence for the localization of inhibin and inhibin-like peptides in Leydig cells of primates [1,4,31,34], rats [17,24,26] and sheep [32]. Furthermore, inhibin α-subunit (Ih-α) messenger RNA (mRNA) was localized in Leydig cells of rats [8,23,24].…”

Section: Animals and Tissue Preparationsmentioning

confidence: 99%

Expression of Inhibin a-Subunit in Horse Testis.

Fujimura

Hondo

Kobayashi

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Inhibin is believed to play roles in the pituitary secretion of FSH and in the paracrine regulation of testicular function. Although it has been generally accepted that inhibin is produced in Sertoli cells, there was a recent evidence for the localization of inhibin in Leydig cells of primates, rat and sheep. However, there is no report on the expression of inhibin in the adult horse testis. Therefore, using immunohistochemistry, western blotting and in situ hybridization techniques, the present study examined inhibin α-subunit (Ih-α) expression in the adult horse testis. For the detection of Ih-α protein, we used anti-porcine Ih-α antibody in immunohistochemistry and western blotting. Furthermore, digoxigenin-labeled complementary RNA probes were prepared to detect intracellular messenger RNA (mRNA) of Ih-α. Immunostainings for Ih-α were found not only in Leydig cells but also in Sertoli cells. The intensity in Leydig cells was stronger than in Sertoli cells. Immunoreactivities for Ih-α were found at approximately 46 kDa, 56 kDa and 90 kDa in the homogenates from testicular interstitial tissues. The bands at 56 kDa and 90 kDa agree with previous report, but not at 46 kDa. Signals for mRNA of Ih-α by in situ hybridization were detected in Leydig cells and in the basal region of seminiferous epithelium including Sertoli cells. These results suggest that Ih-α is expressed in Leydig cells and Sertoli cells of horse testis, and the expression level should be higher in Leydig cells than Sertoli cells. -KEY WORDS: horse testis, inhibin α-subunit, in situ hybridization, Leydig cell, Sertoli cell.

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“…Heterodimers of a distinct -subunit with one of the two -subunits produce either inhibin A ( A ) or inhibin B ( B ). Activin A is produced by Sertoli cells, peritubular cells and Leydig cells from immature testes (Lee et al 1989, de Winter et al 1993, and Sertoli cells appear to produce some activin B (de Winter et al 1993). The Sertoli cells are also the principal source of inhibin B, which is the predominant inhibin isoform in the male .…”

Section: Introductionmentioning

confidence: 99%

Reciprocal regulation of activin A and inhibin B by interleukin-1 (IL-1) and follicle-stimulating hormone (FSH) in rat Sertoli cells in vitro

Okuma

Saito

O’Connor

et al. 2005

Journal of Endocrinology

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In several biological systems, the inhibin A homodimer activin A is stimulated by, and in turn, inhibits the action of interleukin (IL)-1 (both IL-1 and IL-1 ) and IL-6. The possibility that a similar regulatory relationship operates within the testis was investigated. Sertoli cells from immature (20-day-old) rats were cultured with human IL-1 or IL-1 , human IL-6 and/or ovine FSH or dibutyryl cAMP. Activin A and the inhibin dimers, inhibin A and inhibin B, were measured by specific ELISA. Immunoreactive inhibin (ir-inhibin) was measured by RIA. Activin/inhibin subunit mRNA expression was measured by quantitative real-time PCR. Both IL-1 isoforms, but not IL-6, stimulated activin A secretion through increased synthesis of A -subunit mRNA. IL-1 also stimulated activin A secretion by testicular peritubular cells. In contrast to the effect on activin A, IL-1 suppressed inhibin B -subunit and, to a lesser extent, -subunit mRNA expression, thereby reducing basal and FSHstimulated inhibin B secretion by the Sertoli cells. Conversely, FSH inhibited basal activin A secretion and antagonised the stimulatory effects of IL-1. Dibutyryl cAMP partially inhibited the action of IL-1 on activin A secretion, but had no significant effect on basal activin A secretion. Secretion of inhibin A was low in all treatment groups. These data demonstrate that IL-1 and FSH/cAMP exert a reciprocal regulation of activin A and inhibin B synthesis and release by the Sertoli cell, and suggest a role for activin A as a potential feedback regulator of IL-1 and IL-6 activity in the testis during normal spermatogenesis and in inflammation.

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Secretion of Activin by Interstitial Cells in the Testis

Cited by 98 publications

References 19 publications

Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors.

Pituitary-like proopiomelanocortin transcripts in human Leydig cell tumors.

Expression of Inhibin a-Subunit in Horse Testis.

Reciprocal regulation of activin A and inhibin B by interleukin-1 (IL-1) and follicle-stimulating hormone (FSH) in rat Sertoli cells in vitro

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