Secretion of heterologous and native proteins, growth and morphology in batch cultures of
            <i>Aspergillus niger</i>
            B1‐D at varying agitation rates

Wongwicharn, Aporn; Harvey, Linda M.; McNeil, Brian

doi:10.1002/(sici)1097-4660(199908)74:8<821::aid-jctb114>3.0.co;2-#

Cited by 17 publications

(8 citation statements)

References 45 publications

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“…Product formation of filamentous fungi and pellet growth is influenced by pellet size and structure [6,13,25,28,33,34]. Figure 7 displays the dependency of the final product concentration after 80 h cultivation time on energy dissipation.…”

Section: Product Formationmentioning

confidence: 99%

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Agitation effects on submerged growth and product formation of Aspergillus niger

Kelly

Grimm

Hengstler

et al. 2004

Bioprocess Biosyst Eng

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Product formation of mycelial organisms, like Aspergillus niger, is intimately connected with their morphology. Pellet morphology is often requested for product formation. Therefore, it is important to reveal the influence of the hydrodynamic conditions on the morphological development. In the present study, pellet morphology and glucoamylase formation were studied under different agitation intensities of A. niger AB1.13. For pellet formation inside the bioreactor, without the use of precultures, it is necessary to work at low energy dissipation rates. Biomass growth and glucoamylase activity were correlated with energy dissipation. Furthermore, product yield was analysed in dependence of pellet size and concentration. The present work shows that simple equations based on Monod-kinetics can describe growth and product formation, in general, also in mycelian organisms. All measured morphological data, like pellet concentration, as well as glucoamylase formation, strongly depend on the hydrodynamic conditions.

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Section: Product Formationmentioning

confidence: 99%

“…citric acid production [28]. In addition, this strain is known for high secretion capability, resulting in high yields of homologous and heterologous proteins like glucoamylase or antibiotics [2,30,33]. Agitation has two main effects on the cultivation of filamentous fungi in stirred tank reactors.…”

Section: Introductionmentioning

confidence: 99%

Agitation effects on submerged growth and product formation of Aspergillus niger

Kelly

Grimm

Hengstler

et al. 2004

Bioprocess Biosyst Eng

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“…For example, Aspergillus niger is the prototypical fungus for the production of citric acid (Clark, 1962;Lal, 1980;Grewal and Kalra, 1995), homologous proteins (esp. enzymes) and heterologous proteins (Archer et al, 1994;Prasertsan et al, 1997;Radzio and Kuck, 1997;Wongwicharn et al, 1999;Xu et al, 2000). Moreover, investigation, Thanh and Simard (1973) ).…”

Section: Introductionmentioning

confidence: 99%

Utilization of fungi for biotreatment of raw wastewaters

Coulibaly¹,

Gourène²,

Agathos³

2003

Afr. J. Biotechnol.

121

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Fungal biomasses are capable of treating metal-contaminated effluents with efficiencies several orders of magnitude superior to activated carbon (F-400) or the industrial resin Dowex-50. Additionally, fungal biomasses are susceptible to engineering improvements and regeneration of their capabilities. With regard to organic pollutants, excessive nutrients and dyes, fungi can remove them from wastewaters, leading to a decrease in their toxicities. However, the detoxification rates seem to be dependent on media and culture conditions. The postreatement by anaerobic bioprocesses of effluents that have been pretreated with fungi can lead to higher biogas than the original effluents. In addition to the degradation of organic pollutants, fungi produce added-value products such as enzymes (LiP, MnP, Lacc, amylase, etc.) and single-cell protein (SCP). Most research on fungal capacities to purify polluted effluents has been performed on a laboratory scale, hence there is a need to extend such research to pilot scale and to apply it to industrial processes.

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“…In particular, power input or agitation intensity has been identified as an important variable influencing the fungal morphology and productivity in recombinant Aspergillus protein production processes [25,28,[37][38][39]. In general, increasing power input changes the growth morphology from pelleted to filamentous growth forms.…”

Section: Introductionmentioning

confidence: 99%

“…With respect to protein production, there are conflicting results on the effect of agitation intensity. For example, power input greatly influenced morphology and extracellular hen egg white lysozyme production by recombinant Aspergillus niger with highest productivities obtained at intermediate agitation speed in batch culture [37]. On the other hand, production of an extracellular cutinase by recombinant Aspergillus awamori was found to be independent on the agitation intensity in fed-batch cultures [25].…”

Section: Introductionmentioning

confidence: 99%

Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger

El‐Enshasy

Kleine

Rinas

2006

Process Biochemistry

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Recombinant Aspergillus niger genetically engineered to produce glucose oxidase using the constitutive gpdA promoter and the glucoamylase signal sequence for secretion was grown in batch cultures at agitation speeds of 200 -800 rpm covering the industrial relevant power input range of 0.1 -5 W kg -1 . The growth morphology ranged from large pellets with an average diameter of 1500 µm at low power input up to micropellets embedded in a filamentous network at high power input. A correlation of agitation intensity with growth morphology and glucose oxidase production revealed an increase of the protein production capability with the change of the growth morphology from pelleted to filamentous growth forms. However, the exposure to higher shear stress with increasing power input also resulted in lower biomass yields as well as increased transient formation of polyol (xylitol) and higher final concentrations of oxalic acid. The highest specific production rates were found in young filamentous growth forms at high power input. Although intermediate agitation intensity leading to small pellets became more favorable during prolonged cultivation. An acridine orange staining procedure discriminating between RNA rich (red) and RNA poor regions (green) of the fungal biomass proved that active protein production is restricted to filamentous growth forms and the outer layer of fungal pellets. A correlation between the RNA rich fraction of the biomass determined by image analysis and the productivity is shown.

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Secretion of heterologous and native proteins, growth and morphology in batch cultures of Aspergillus niger B1‐D at varying agitation rates

Cited by 17 publications

References 45 publications

Agitation effects on submerged growth and product formation of Aspergillus niger

Agitation effects on submerged growth and product formation of Aspergillus niger

Utilization of fungi for biotreatment of raw wastewaters

Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger

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