Secretome of Cultured Human Endothelial Cells in Simulated Microgravity

Каширина, Д. Н.; Кононихин, А. С.; Ларина, И. М.; Буравкова, Л. Б.

doi:10.1007/s10517-019-04454-8

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Via complex formation, the SD of BCAR1 influences various small GTPases [54,71]. In recent years, a number of studies have been published, which show that microgravityinduced changes of small GTPase activity play a role in the adjustment of various cells to altered gravity [122], e.g., of endothelial cells [123], glioma cells [124], stem cells [125] and cancer cells [126]. The changes of small GTPase activities accompany alterations of adhesion, migration and cytoskeletal rearrangements [124,[126][127][128] as well as cell differentiation and morphogenesis [125,129].…”

Section: Discussionmentioning

confidence: 99%

Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity

Bauer¹,

Gombocz²,

Schulz

et al. 2021

Computation

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When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.

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Section: Discussionmentioning

confidence: 99%

Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity

Bauer¹,

Gombocz²,

Schulz

et al. 2021

Computation

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show abstract

“…Significant changes in proteins involved in the hemostasis system and post-translational changes, particularly in phosphorylation, have been demonstrated. The same scientific team studied the effects of microgravity on an endothelial cell culture [ 92 ] and found changes in certain elements of the cytoskeletal structure by a complex regulatory system involving Rho proteins. All these results were obtained after submitting cell extracts with CPLL to enrich low-abundance proteins.…”

Section: Other Medical Involvementsmentioning

confidence: 99%

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Boschetti¹,

Righetti

2023

IJMS

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The discovery of low- and very low-abundance proteins in medical applications is considered a key success factor in various important domains. To reach this category of proteins, it is essential to adopt procedures consisting of the selective enrichment of species that are present at extremely low concentrations. In the past few years pathways towards this objective have been proposed. In this review, a general landscape of the enrichment technology situation is made first with the presentation and the use of combinatorial peptide libraries. Then, a description of this peculiar technology for the identification of early-stage biomarkers for well-known pathologies with concrete examples is given. In another field of medical applications, the determination of host cell protein traces potentially present in recombinant therapeutic proteins, such as antibodies, is discussed along with their potentially deleterious effects on the health of patients on the one hand, and on the stability of these biodrugs on the other hand. Various additional applications of medical interest are disclosed for biological fluids investigations where the target proteins are present at very low concentrations (e.g., protein allergens).

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“…These data are important to try preparing the parade for future long trips. The same team made another investigation around the secretome of endothelial cells cultured in vitro under microgravity [97]. After the enhancement of minor proteins by CPLL, it was found that certain elements of the cellular structure, such as microtubules and associated regulation proteins, have been affected.…”

Section: Acceptance Of Cpll Technology Over Time and Major Driversmentioning

confidence: 99%

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Boschetti¹,

Zilberstein

Righetti

2021

Electrophoresis

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After a decade of experimental applications, it is the objective of this review to make a point on combinatorial peptide ligand libraries dedicated to low‐abundance proteins from animals to plants and to microorganism proteomics. It is, thus, at the light of the recent technical developments and applications that we will examine the state of the art, its usage within the scientific community, and its openness to unexplored fields. The improvements of the methodology and its implementation in connection with analytical determinations of combinatorial peptide ligand library (CPLL)‐treated samples are extensively reviewed and commented upon. Relevant examples covering few critical aspects describe the performance of the technology. Finally, a reflection on the technological future is attempted in particular by involving new concepts adapted to the limited availability of certain biological samples.

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Secretome of Cultured Human Endothelial Cells in Simulated Microgravity

Cited by 6 publications

References 14 publications

Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity

Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Combinatorial peptides: A library that continuously probes low‐abundance proteins

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