Seed Germination Enhancement in High Altitude Medicinal Plants of Garhwal Himalaya by Some Pre-sowing Treatments

Prakash, Vinay; Bisht, Hemlata; Nautiyal, M. C.

doi:10.3923/rjss.2011.199.205

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…The addition of GA 3 was found more effective to increase seeds germination compare to MS with lack of GA 3 (MS 0). This result is similar to other studies on several species that the application of GA 3 is an effective method to promote germination and to increase the germination capability (Perez-Garcia and Duran 1990, Upreti and Dhar 1996, Khan and Ungar 1998, Hernandez-Verdugo et al, 2001, Miransari and Smith 2009, Prakash et al, 2011. Furthermore, Akhtar et al, (2008) reported that the addition of GA 3 were able to increase the potential embryo growth and endosperm weakening, cell elongation, cell division, and to promote growth and development of many plant species.…”

Section: Resultssupporting

confidence: 90%

In vitro plant regeneration from hypocotyl of Arben (Rubus fraxinifolius Poir.)

Ismaini¹,

Surya²,

Destri³

2017

Aust J Crop Sci

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Rubus fraxinifolius, a group of wild raspberry, was improved as a fresh fruit in Indonesia. The purpose of this study was to develop a protocol for micropropagation of R. fraxinifolius. Cultures were subcultured from hypocotyls (1 cm) which is initiated from seeds. The experiments were focused on the effect of plant growth regulators concentrations and combinations. The highest germination by in vitro was obtained using MS medium supplemented with 10 mg/L of GA 3. Furthermore, based on five parameters, i.e. callus height, callus diameter, number of leaves, plantlet height and number of roots, medium 3 (0.5 mg/L GA 3 , 2.5 mg/L 2-iP and 0.1 mg/L NAA) shown the best results during the development explants of R. fraxinifolius. Effectivity of R. fraxinifolius regeneration through in vitro was affected by 2-iP and NAA concentration. If both of plant growth regulators were high (medium 8) or low (medium 1), it made less growth.

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Section: Resultssupporting

confidence: 90%

In vitro plant regeneration from hypocotyl of Arben (Rubus fraxinifolius Poir.)

Ismaini¹,

Surya²,

Destri³

2017

Aust J Crop Sci

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“…Similar kind of results was obtained by Yousef and Mahmood (2014) on Bunium persicum an important medicinal aromatic plant species of Himalaya. Seeds of alpine plant species mostly hydrated ones commonly germinate when exposed to low temperature (Prakash et al 2011). The overexploitation of important plant species is the basic reason for their extinction.…”

Section: Resultsmentioning

confidence: 99%

Efficient Propagation Methods and Management Implication of Aconitum chasmanthum Stapf ex Holmes: A Critically Endangered and Endemic Medicinal Herb of Kashmir Himalaya

Mohi-Ud-Din¹,

Nawchoo²,

Wafai³

2022

IJARe

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Background: Knowledge about propagation of rare and endangered plant species is very important for their conservation and sustainable utilization. Therefore, it is imperative to understand different reproductive aspects of such species for their conservation and management. It is in this backdrop different propagation method of Aconitum chasmanthum- a Critically Endangered and Endemic Medicinal plant species has been undertaken. Methods: Seed germination was carried out using different media. For each treatment mean germination time (MGT) was also calculated. Different agro-techniques were also developed in different soil textural classes. The fresh underground tubers of the species were collected from natural population and were split longitudinally into 2, 3 and 4 pieces depending upon the size of the parental tuber containing a portion of shoot apex. The split cuttings were treated with 100 ppm IAA, IBA and GA3 for 48 hours and were sown in sandy loam soils under pots at Herbal Garden. The pots were kept in diffused light to monitor the leafy shoot generation, survival and comparison with the control (untreated cuttings). Result: The present study revealed that seeds of the species remain dormant for a pretty long period and do not germinate in darker conditions. Among various treatments the species showed 90.0±8.66% germination when chilled for 20 days with mean germination time (MGT) of 13.678±1.874 days as against 10.66±1.154% germination of the control. Although the plants grew nicely on various soils, maximum plant survival and vigorous growth was obtained in loamy textured soils. The plants responded to various nutrient treatments and in loamy soil the maximum dry biomass was shown by the plants treated with nitrogen (300 mg/kg soil) and NPK (500 mg/kg soil). The plants treated with NPK (500 mg/kg soil) produced 14.46±2.50 gm dry biomass as against the control of 5.23±0.750 gm per individual. The experimental manipulations also reveal that the species has a potential to propagate through tuber cuttings in sandy loam soils. The cuttings treated with IAA, IBA and GA3 showed 68.75% to 75.0% survival rate as against the 62.5% in control.

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“…They achieved 76.76% seed germination on MS basal medium when seeds were stratified at 5±1°C for 21 days and 100 ppm GA3 treatment for 24 hours. Prakash et al (2011) observed enhancement in seed germination of P. verticillatum by giving chilling treatment (at -20°C for 15 to 60 days and exogenous application of 100 ppm GA3) before sowing in soil. Toma and Rasheed (2012) achieved highest in vitro seed germination of Asparagus Densiflorus L. by giving the seeds stratification prior to culture.…”

Section: A In Vitro Seed Germinationmentioning

confidence: 87%

“…1b). For in vitro studies, seeds were given pre culture treatment as adopted by Prakash et al 2011 with some modifications. For in vitro seed germination, seeds were stratified at different temperatures (4ºC, -20ºC, -80 ºC) for different time periods (10, 20 and 30 days) along with 1mg/l gibberellic acid (GA3) solution, but seeds stratified at -20ºC for 30 days along with GA3 (1mg/l) solution were used because this was the only pre culture treatment which responded to in vitro growth conditions.…”

Section: A Plant Materialsmentioning

confidence: 99%

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

Qadir¹,

Singh²,

Kour³

et al. 2020

JSR

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To conserve and overcome the limitations of traditional propagation, a need was felt to develop an in vitro propagation protocol for Polygonatum verticillatum (L.) All. by using seeds as explant. The present study was undertaken to evaluate the most suitable concentration of plant growth regulators on callus induction and subsequent rhizome and shoot regeneration. Murashige and Skoog (MS) medium fortified with Kn (8mg/l) in combination with IAA (2mg/l) proved most effective in producing maximum amount of callus from in vitro sprouted seed explants which were pre-treated with GA3 (1 mg/l) and kept at-20 º C for 30 days. Subculturing of callus on MS medium fortified with BAP (4mg/l) and NAA (1mg/l) in combination regenerated rhizomes. Shoots regenerated (13.25±2.32) from rhizomes on MS medium fortified with Kn (2mg/l) in combination with BAP (2mg/l). Roots (1.93±0.34 cm) regenerated from shoots inoculated on full strength MS medium fortified with IBA at a concentration of (2.0 mg/l) within 90 days. These in vitro raised shoots with well developed roots were successfully acclimatized in vermicompost with 80% survival rate. In addition to in vitro shooting, ex vitro regeneration of shoots from in vitro raised rhizomes was also achieved in vermicompost within 49 days.

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Seed Germination Enhancement in High Altitude Medicinal Plants of Garhwal Himalaya by Some Pre-sowing Treatments

Cited by 8 publications

References 14 publications

In vitro plant regeneration from hypocotyl of Arben (Rubus fraxinifolius Poir.)

In vitro plant regeneration from hypocotyl of Arben (Rubus fraxinifolius Poir.)

Efficient Propagation Methods and Management Implication of Aconitum chasmanthum Stapf ex Holmes: A Critically Endangered and Endemic Medicinal Herb of Kashmir Himalaya

In vitro Propagation of Polygonatum verticillatum All. A Threatened Medicinal Herb through Seed Explant

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