Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 pathotype Globodera rostochiensis in selected F&lt;sub&gt;1&lt;/sub&gt; progeny

Skupinová, S.; Vejl, P.; Sedlák, Petr; Domkářová, J.

doi:10.17221/4399-pse

Cited by 8 publications

(11 citation statements)

References 16 publications

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“…In other studies, marker CP113 showed either weak (Niewöhner et al, 1995) or good (Skupinová et al, 2002) diagnostic suitability. The markers U14 and X02 linked to the gene GroV1, marker 77R linked to Gpa2, marker TG432 associated with QTL conferring resistance to G. pallida and the SNP marker HC were unsuitable for diagnostic Markers 77R and HC are not presented because they were not detected in the parental forms of these tested clones purposes.…”

Section: Discussionmentioning

confidence: 76%

“…Nowadays the most widely used are CAPS and SCAR markers (Barone 2004). The following markers are recommended as a good tool for selection genotypes resistant to PCN: U14 and X02 (Jacobs et al 1996), CP113 (Niewöhner et al 1995, Skupinová et al 2002, TG689 (Sagredo et al 2004;Biryukova et al 2008), Gro 1,4 (Paal et al 2004;Gebhardt et al 2006), 77R (van der Voort et al 1999, SPUD1636 (Bryan et al 2002) and SNP marker named HC (Sattarzadeh et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

2011

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Globodera rostochiensis and Globodera pallida are parasitic root cyst nematodes of potato (PCN), which significantly reduce yield and quality. The genetics and available molecular markers should make resistance to nematodes an excellent candidate for marker assisted selection. The study presents results of testing the suitability of known molecular markers for detection of resistance in a set of cultivars. We revealed some inconsistencies in genealogical data of the cultivars and showed inconsistent usefulness in detecting resistant cultivars. The marker TG689 was identified in almost all cultivars resistant to G. rostochiensis and together with other markers was used for verifying the resistance in a group of breeding lines. The marker TG689 was the most effective. However, the efficiency of tested markers in selecting resistant individuals might be inadequate. In the case of quantitative resistance to G. pallida, marker SPUD1636 was found in resistant cultivars, and not in susceptible cultivars, but turned out to be inefficient in selecting resistant breeding lines.Resumen Globodera rostochiensis y Globodera pallida son nematodos de quiste parásitos de la raíz de la papa (PCN) que reducen significativamente el rendimiento y la calidad. La genética y los marcadores moleculares disponibles, deberían de hacer de la resistencia a nematodos un excelente candidato para selección asistida con marcadores. El estudio presenta resultados de probar lo deseable de los marcadores moleculares conocidos para la detección de resistencia en un grupo de variedades. Revelamos algunas inconsistencias en datos genealógicos de las variedades y mostramos uso inconsistente en la detección de variedades resistentes. El marcador TG689 se identificó en casi todas las variedades resistentes a G. rostochiensis y junto con otros marcadores se usó para verificar la resistencia en un grupo de líneas de mejoramiento. El marcador TG689 fue el más efectivo. No obstante, podría ser inadecuada la eficiencia de los marcadores probados en la selección de individuos resistentes. En el caso de resistencia cuantitativa a G. pallida, se encontró el marcador SPUD1636 en variedades resistentes y no en los susceptibles, pero resultó ser ineficiente en la selección de líneas de mejoramiento resistentes.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

2011

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“…Penelitian yang dilakukan oleh Colton et al (2006) menunjukkan bahwa dari total progeni kentang yang dievaluasi ketahanannya terhadap penyakit hawar daun, menghasilkan 25-50% progeni yang positif mengandung gen RB, yang berarti menunjukkan rasio segregasi dari 1 : 3 sampai 1 : 1. Segregasi marka DNA H1 pada tanaman kentang untuk ketahanan terhadap patotipe Ro1 Globodera rostochiensis pada progeni F 1 Tabor x Mira menunjukkan 50,22% mengandung gen H1 dan 49,78% tidak mengandung gen H1, sedangkan persilangan Ornella x Mira menghasilkan 56,16% progeni F 1 dengan gen H1 dan 43,84% tidak membawa gen H1 (Skupinova et al 2002).…”

Section: Analisis Pcr Gen Rbunclassified

Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik

Ambarwati¹,

Purwito²,

Herman³

et al. 2016

J. AgroBiogen

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Integration and Segregation Analysis of Late Blight Resistance Gene in F 1 Progenies of Transgenic and Non Transgenic Potato Crosses. A. Dinar Ambarwati, Agus Purwito, M. Herman, S.M. Sumaraow, and HajrialAswidinnoor. Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 and SP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F 1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%) berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F 1 progenies were analyzed for the presence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F 1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, and Granola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio. Gambar 2. Hasil amplifikasi PCR (N-term end) untuk deteksi integrasi gen RB pada progeni F1 Atlantic x transgenik Katahdin SP904. M = 1 Kb DNA ladder, 1-19 = progeni F1 Atlantic x Katahdin SP904, 20 = tetua tahan (Katahdin SP904), 21 = tetua peka (Atlantic), 22 = H2O. Gambar 3. Hasil amplifikasi PCR (N-term end) untuk deteksi integrasi gen RB pada progeni F1 Atlantic x transgenik Katahdin SP951. M = 1 Kb DNA ladder, 1-19 = progeni F1 Atlantic x Katahdin SP951, 20 = tetua tahan (Katahdin SP951), 21 = tetua peka (Atlantic), 22 = H2O. Gambar 4. Hasil amplifikasi PCR (N-term end) untuk deteksi integrasi gen RB pada progeni F1 Granola x transgenik Katahdin SP904. M = 1 Kb DNA ladder, 1-19 = progeni F1 Granola x Katahdin SP904, 20 = tetua tahan (Katahdin SP904), 21 = tetua peka (Granola), 22 = H2O. 619 bp M 1 2 3 4 5

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“…One of the earliest markers to be developed for the H1 resistance gene, CP113, (Gebhardt et al. 1993) produced variable results, providing an effective predictor of PCN resistance within some germplasm sources (Skupinova et al. 2002, Dale et al.…”

mentioning

confidence: 99%

“…Unfortunately, to date, all markers identified as linked to the H1 resistance gene have displayed incomplete association of diagnostic alleles. One of the earliest markers to be developed for the H1 resistance gene, CP113, (Gebhardt et al 1993) produced variable results, providing an effective predictor of PCN resistance within some germplasm sources (Skupinova et al 2002, Dale et al 2006), but not others (Niewohner et al 1995, Hutchinson 1997, Milczarek et al 2011.…”

mentioning

confidence: 99%

Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)

et al. 2012

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Potato cyst nematode (PCN) is a major pest of potato crops. Markerassisted selection of PCN-resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker of choice for the prediction of PCN resistance. Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCNresistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452-bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. To enable routine implementation, the 57R marker was converted into a high-throughput, fluorochrome detection-based assay and screened across a broader collection of parental cultivars and advanced breeding selections for the prediction of PCN resistance.

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Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 pathotype Globodera rostochiensis in selected F<sub>1</sub> progeny

Cited by 8 publications

References 16 publications

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik

Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)

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