Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases

Hong-yan, Yang; Wu, Hao; Wang, Xiaofen; Cui, Zongjun; Li, Yuhua

doi:10.1016/j.biortech.2010.09.009

Cited by 46 publications

(28 citation statements)

References 26 publications

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“…This is likely a result of the lack of feedback regulation and metabolite repression that commonly occurs in single strain fermentation (Haruta et al, 2002). In fact, several studies have directly demonstrated the efficiency of constructed microbial consortia in the hydrolysis of lignocellulose (Guo et al, 2011;Haruta et al, 2002;Wongwilaiwalin et al, 2010;Yang et al, 2011). However, to our knowledge such microbial consortia have not been directly used in the pretreatment of Napier grass.…”

Section: Methane Yield Of Treated and Untreated Napier Grassmentioning

confidence: 95%

“…In fact, it has been approved that the complete decomposition of lignocellulose requires the combined actions of a diverse range of microorganisms (Slater and Lovatt, 1984). Several studies have demonstrated the efficiency of constructed microbial consortia for the rapid degradation of lignocellulose (Haruta et al, 2002;Wongwilaiwalin et al, 2010;Yang et al, 2011). To our knowledge, such microbial consortia have not been directly used in the pretreatment of Napier grass.…”

Section: Introductionmentioning

confidence: 95%

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Comparison and evaluation of concurrent saccharification and anaerobic digestion of Napier grass after pretreatment by three microbial consortia

Wen

Yuan

et al. 2015

Bioresource Technology

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Section: Methane Yield Of Treated and Untreated Napier Grassmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 95%

Comparison and evaluation of concurrent saccharification and anaerobic digestion of Napier grass after pretreatment by three microbial consortia

Wen

Yuan

et al. 2015

Bioresource Technology

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“…In previous studies, several microbial communities have been developed to convert lignocellulose effectively to biofuels (30,35,36). However, the communities were so complex that it was difficult to study how the strains collaborated with one another.…”

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confidence: 99%

Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose

Zhang

Chen

Schwarz

et al. 2014

Appl Environ Microbiol

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c Two cellulolytic thermophilic bacterial strains, CS-3-2 and CS-4-4, were isolated from decayed cornstalk by the addition of growth-supporting factors to the medium. According to 16S rRNA gene-sequencing results, these strains belonged to the genus Clostridium and showed 98.87% and 98.86% identity with Clostridium stercorarium subsp. leptospartum ATCC 35414 T and Clostridium cellulosi AS 1.1777 T , respectively. The endoglucanase and exoglucanase activities of strain CS-4-4 were approximately 3 to 5 times those of strain CS-3-2, whereas the ␤-glucosidase activity of strain CS-3-2 was 18 times higher than that of strain CS-4-4. The xylanase activity of strain CS-3-2 was 9 times that of strain CS-4-4, whereas the ␤-xylosidase activity of strain CS-4-4 was 27 times that of strain CS-3-2. The enzyme activities in spent cultures following cocultivation of the two strains with cornstalk as the substrate were much greater than those in pure cultures or an artificial mixture of samples, indicating synergism of glycoside hydrolase secretomes between the two strains. Quantitative measurement of the two strains in the cocultivation system indicated that strain CS-3-2 grew robustly during the initial stages, whereas strain CS-4-4 dominated the system in the late-exponential phase. Liquid chromatography-tandem mass spectrometry analysis of protein bands appearing in the native zymograms showed that ORF3880 and ORF3883 from strain CS-4-4 played key roles in the lignocellulose degradation process. Both these open reading frames (ORFs) exhibited endoglucanase and xylanase activities, but ORF3880 showed tighter adhesion to insoluble substrates at 4, 25, and 60°C owing to its five carbohydrate-binding modules (CBMs).

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“…is commonly isolated from the human gastrointestinal tract as a causative pathogen of various infections (Park et al 2012), some Achromobacter species are also confirmed to exist in the cellulose-degrading bacterial community by means of 16S rDNA comparison or fatty acid analysis (Dumova and Kruglov 2009). However, there was no single colony to be isolated from the cellulolytic coenosis so far, as well as the function of those strains was not clarified (Dumova and Kruglov 2009;Yang et al 2011;Talia et al 2012). Recently an Achromobacter species was found to be always associated with the cellulolytic Cytophagales in our searching for cellulose-degrading microbes.…”

Section: Discussionmentioning

confidence: 95%

Isolation and characterization of Achromobacter sp. CX2 from symbiotic Cytophagales, a non-cellulolytic bacterium showing synergism with cellulolytic microbes by producing β-glucosidase

et al. 2014

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A Gram-negative, obligately aerobic, noncellulolytic bacterium was isolated from the cellulolytic association of Cytophagales. It exhibits biochemical properties that are consistent with its classification in the genus Achromobacter. Phylogenetic analysis together with the phenotypic characteristics suggest that the isolate could be a novel species of the genus Achromobacter and designated as CX2 (= CGMCC 1.12675=CICC 23807). The strain CX2 is the symbiotic microbe of Cytophagales and produces β-glucosidase. The results showed that the non-cellulolytic Achromobacter sp. CX2 has synergistic activity with cellulolytic microbes by producing β-glucosidase. To our knowledge, this is the first report on the synergetic effect of the combination of noncellulolytic and cellulolytic microbes, which is significant to help understand the cellulolytic mechanism of cellulosedigesting Cytophagales.

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Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases

Cited by 46 publications

References 26 publications

Comparison and evaluation of concurrent saccharification and anaerobic digestion of Napier grass after pretreatment by three microbial consortia

Comparison and evaluation of concurrent saccharification and anaerobic digestion of Napier grass after pretreatment by three microbial consortia

Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose

Isolation and characterization of Achromobacter sp. CX2 from symbiotic Cytophagales, a non-cellulolytic bacterium showing synergism with cellulolytic microbes by producing β-glucosidase

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