Selection and characterization of DNA aptamers for constructing colorimetric biosensor for detection of PBP2a

Fan, Yaofang; Cui, Mengyu; Liu, Yanming; Jin, Minli; Zhao, Huimin

doi:10.1016/j.saa.2019.117735

Cited by 21 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infections. Colorimetric biosensors are sensitive, selective, and low-cost detection tools capable of detecting analytes based on color changes that can be easily recognized by naked eyes or simple portable optical detectors [157][158][159]. Special physicochemical properties of NPs have led to emergence of new colorimetric diagnostic systems [160].…”

Section: Nanotechnology In Colorimetric Detection Of Coronavirusmentioning

confidence: 99%

Recent Advances on Nanotechnology-Based Strategies for Prevention, Diagnosis, and Treatment of Coronavirus Infections

Alimardani

Abolmaali

Tamaddon

2021

Journal of Nanomaterials

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is exponentially spreading across the world, leading to an outbreak of serious viral pneumonia. Antiviral therapies using chloroquine, hydroxychloroquine, and favipiravir have been approved by several countries to increase the quality of life of SARS-CoV-2-infected patients. Currently, several companies are intensively working on the production of coronavirus (CoV) vaccines, resulting in some specific vaccines that have been approved for CoV infections in humans. Nevertheless, efficient and specific prevention, treatment, and diagnosis are urgently required to combat the biological diversity and rapid mutation in CoV infections. Recently, significant attention has devoted to nanoformulation or nanoparticles (NPs) due to their specific features like high surface-to-volume ratio, drug encapsulation abilities, and specific optical properties to remove the complications of applied conventional therapeutic and diagnosis options. In this regard, NPs are increasingly used as new anti-CoV agents, vaccine carriers or adjuvants, and nanoscale biorecognition elements. The present review article provides a comprehensive discussion on the recent updates regarding the prevention, diagnosis, and treatment of different CoV infections with an emphasis on the application of NPs in vaccination, treatment, and diagnosis of CoV infections.

show abstract

Section: Nanotechnology In Colorimetric Detection Of Coronavirusmentioning

confidence: 99%

Recent Advances on Nanotechnology-Based Strategies for Prevention, Diagnosis, and Treatment of Coronavirus Infections

Alimardani

Abolmaali

Tamaddon

2021

Journal of Nanomaterials

View full text Add to dashboard Cite

show abstract

“…The effective diagnosis of pathogenic bacteria is of great significance in the process of disease prevention, control, and diagnosis, in order to meet the needs of rapid diagnosis of methicillin-resistant Staphylococcus aureus (MRSA). A research group coupled the MRSA marker protein penicillin binding protein (PBP2a) aptamer to graphene oxide-loaded gold nanoparticles (GO/Au) [163]. Preliminary experiments proved that GO/Au has H 2 O 2 enzymatic activity, which can catalyze the conversion of TMB to make the solution blue and significantly increase the catalytic activity after binding to ssDNA.…”

Section: Functional Nucleic-acid Biosensors Design For Pathogen Detectionmentioning

confidence: 99%

“…Therefore, when the aptamer is not bound to the target, the spontaneous combination of the aptamer and GO/Au increases the H 2 O 2 enzyme activity and makes the solution blue due to the π-π stacking effect. The color of the system changes according to the concentration of the target [163]. A sensor prepared in this way can detect at least 20 nM of PBP2a.…”

Section: Functional Nucleic-acid Biosensors Design For Pathogen Detectionmentioning

confidence: 99%

Recent Advances on Functional Nucleic-Acid Biosensors

Zhang

Guo

et al. 2021

Sensors

View full text Add to dashboard Cite

In the past few decades, biosensors have been gradually developed for the rapid detection and monitoring of human diseases. Recently, functional nucleic-acid (FNA) biosensors have attracted the attention of scholars due to a series of advantages such as high stability and strong specificity, as well as the significant progress they have made in terms of biomedical applications. However, there are few reports that systematically and comprehensively summarize its working principles, classification and application. In this review, we primarily introduce functional modes of biosensors that combine functional nucleic acids with different signal output modes. In addition, the mechanisms of action of several media of the FNA biosensor are introduced. Finally, the practical application and existing problems of FNA sensors are discussed, and the future development directions and application prospects of functional nucleic acid sensors are prospected.

show abstract

“…Presence of PBP2a DNA in test sample induces change in peroxidase activity of GO/Au due to release of PBP2a -specific aptamer from AuNP-GO surface. This ultimately leads to variation in the UV-visible absorption intensity [ 81 ]. The recovery of fluorescence intensity is used to quantify rifampicin-resistant Mycobacterium tuberculosis .…”

Section: Detection Of Gmo Mutated and Resistant Organismsmentioning

confidence: 99%

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Kumar

Guleria

2020

Curr Pollution Rep

View full text Add to dashboard Cite

Selection and characterization of DNA aptamers for constructing colorimetric biosensor for detection of PBP2a

Cited by 21 publications

References 35 publications

Recent Advances on Nanotechnology-Based Strategies for Prevention, Diagnosis, and Treatment of Coronavirus Infections

Recent Advances on Nanotechnology-Based Strategies for Prevention, Diagnosis, and Treatment of Coronavirus Infections

Recent Advances on Functional Nucleic-Acid Biosensors

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Contact Info

Product

Resources

About