Selection of Pineapple Cultivars Resistant to Fusariose

Cabral, J. R. S.; Matos, A. P. de; Cunha, G. A. P. da

doi:10.17660/actahortic.1993.334.5

Cited by 5 publications

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“…An initial screening of plants is carried out during this cycle, and plants are selected on the basis of such factors as presence of leaf spines, leaf color, growth vigor, fruit shape, size and internal quality. The selected plants are cloned using their slips, hapas and suckers and go through new growing cycles for further selection and confirmation of their genetic characters (CABRAL et al, 1993).…”

Section: Sexual Propagation For Breeding Purposesmentioning

confidence: 99%

Advances in pineapple plant propagation

et al. 2018

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Pineapple is one of the most important fruits, with large production in tropical and subtropical regions and great appreciation by consumers all over the world. The pineapple plant has many specific morphological, anatomical and physiological characteristics that determine crucial aspects of pineapple crop management, such as flower induction, water use and vegetative methods of propagation. The use of sexual reproduction of pineapple is restricted to breeding purposes carried out by research institutes looking for new hybrids with improved agronomic characteristics. Seeds are only produced if cross pollination among varieties occurs. Commercially pineapple has to be propagated by vegetative material, an asexual reproduction, without new combinations of genes. Some types of propagules are naturally produced by the plants and called conventional planting material. Its availability and quality depend on many factors, especially cultivar and environment. Management techniques of this material have been continuously developed and will be addressed. In addition to the conventional planting material, which in many situations is not sufficient to assure expansion or at least maintenance of the cultivated area, several other methods of vegetative propagation of pineapple have been studied and made available along the last decades and will also be discussed, involving techniques of stem sectioning, apical growing point gouging and chemical treatment for transformation of flowers into plantlets. Stem sectioning has been especially interesting, as it is mostly done using plant residues available at low cost, and is a rather simple method suited for multiplication and production of disease-free planting material in nurseries. Gouging and chemical treatment are less practiced, but can be applied in ratoon crops, thereby avoiding the loss of the first cycle fruit. Chemical treatment usually results in rather small plantlets, that must be further grown in nurseries before planting them in the field. And finally micropropagation will also be focused, as in vitro production of plantlets is a very important method of multiplication of new pineapple varieties, but this method yet has not been transformed into a common commercial way of pineapple propagation due to the final high cost and to the still high risks of incidence of somaclonal variations among the plantlets produced.

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Section: Sexual Propagation For Breeding Purposesmentioning

confidence: 99%

Advances in pineapple plant propagation

et al. 2018

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“…Within the elongation medium, the shoots exhibited approximately 8.5 cm of height independently of the explant class size ( Means with the same letter within a same row (capital letter) or a same column (minuscule letter) are not significantly different according to the Student-Newman-Keuls mean separation test (* significant at P < 0.05, ** significant at P < 0.01). 1 Data transformed for analysis using log(x + 2).…”

Section: Shoot Multiplication and Elongationmentioning

confidence: 99%

“…The fusariosis, or gomosis, (Fusarium subglutinans WR) is disseminated through the cutting type slips and suckers, and causes losses of more than 30% of the Brazilian production. The efficient ways to control this disease are the use of plantlets free of the pathogen or resistant cultivars [1][2][3]. Thus, micropropagation techniques are valuable tools for mass clonal propagation of superior clones or varieties.…”

Section: Introductionmentioning

confidence: 99%

Improving pineapple micropropagation protocol through explant size and medium composition manipulation

Vesco¹,

Pinto²,

Zaffari³

et al. 2001

Fruits

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Improving pineapple micropropagation protocol through explant size and medium composition manipulation.Abstract -Introduction. Although several pineapple micropropagation protocols have already been published, significant improvement could be achieved if the stages of the in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Materials and methods. Axillary buds were inoculated on an MS liquid culture medium added with NAA (2 µM) and BAP (4 µM). Regenerated shoots, divided into three classes of different sizes, were then used in further experiments. First, these shoots were inoculated in flasks containing the same MS culture medium with or without growth regulators. Then, four basic media, containing different salts and free from growth regulators were tested. In a third assay, the MS culture medium was compared with a half-diluted MS culture medium for studying the plantlet elongation and rooting stage. Results. In the MS culture medium supplemented with NAA and BAP, the highest multiplication rate (13.5 shoots) was obtained with the smallest shoots inoculated, while in the MS culture medium free of growth regulators, the highest plantlets (7.7 cm) were the result of the highest shoots inoculated and showed no vitrification. The normal MS culture medium, in comparison with the half-diluted one and the three other salt formulations, revealed a significant increase in the plantlet elongation and best general features. For acclimatization, the highest values of the survival rate (93.8%) and fresh and dry weights were obtained with the transference of higher than 7.0 cm in vitro plantlets. Conclusion. Using the protocol described in this work, it is possible to obtain 1 million in vitro plantlets after 9 months from a single bud, with a 45 day subculture interval and an average multiplication rate of 10 shoots per bud.Brazil / Ananas comosus / plant propagation / micropropagation / in vitro experimentation / culture media / transplanting / plant establishment Amélioration du protocole de micropropagation de l'ananas par modification de la taille de l'explant et de la composition du milieu de culture.Résumé -Introduction. Bien que plusieurs protocoles de micropropagation d'ananas aient déjà été publiés, une amélioration significative pourrait être réalisée si les étapes de la culture in vitro étaient mieux définies. Nos travaux ont porté sur plusieurs expérimentations visant la fabrication en série de plantules de haute qualité. Matériel et méthodes. Des bourgeons axillaires ont été inoculés sur un milieu de culture MS liquide additionné d'ANA (2 µM) et de BAP (4 µM). Les pousses régénérées, réparties en trois classes de tailles différentes, ont alors été utilisées pour d'autres expérimentations. Elles ont d'abord été mises en culture dans des flacons contenant le même milieu de culture MS avec ou sans régulateurs de croissance. Puis quatre milieux de base, contenant des sels différents et exempts de régulateurs de croissance, ont été tes...

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“…One of the main objectives was to develop pest and disease resistance in Smooth Cayenne [2]. Many other countries have also started hybridization programmes to develop high-yielding varieties with specific adaptation to their own environments, for instance, in Taiwan [3,4], Malaysia [5][6][7], The Philippines, Cote d'Ivoire, Puerto Rico, Cuba and Australia [8][9][10][11][12][13][14][15]. The varieties obtained recently by hybridization programs in Brazil (Ajubá, Imperial), Australia (Aus-Carnival and Aus-Jubilee), USA (Honey Gold and MD2), Martinique (FLHORAN41) and Malaysia (Josapine) [16].…”

Section: Introductionmentioning

confidence: 99%

New Pineapple Somaclonal Variants: P3R5 and Dwarf

Pérez¹,

Yanez²,

Mbogholi³

et al. 2012

AJPS

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The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We obtained two new pineapple somaclonal variants derived from in vitro culture of cv. Red Spanish Pinar: P3R5 and Dwarf. The AFLP analysis revealed an existing genetic distance. So far 44 phenotype indicators selected due to their relation to a wide range of important agricultural, morphological and physiological processes have been evaluated. P3R5 differed from the donor in 19 variables (19/44; 43.18%), while Dwarf varied in 31 indicators (31/44; 70.45%). The number of shoots was significantly different among the three plant materials. Dwarf showed two shoots per plant while P3R5 and the donor did not form any shoots. We also observed that water use efficiency, chlorophyll b concentration, total chlorophyll concentration, transpiration rate, chlorophyll a concentration, thickness of leaf photosynthetic parenchyma, fruit mass with crown, content of free phenolics and superoxide dismutase specific activity were also very different among the three plant materials. The Euclidean distances of each somaclonal variant to the donor plant material taking into consideration the genotype (AFLP) and the phenotype evaluations were also calculated. Regarding the genotype information, P3R5 is separated from cv. Red Spanish Pinar by 2.83 units of Euclidean distance, and Dwarf by 3.00 units. However, the phenotype indicators revealed higher differences: 3.74 in P3R5 and 4.71 in Dwarf. To our knowledge, this is the first report of a comprehensive analysis of pineapple somaclonal variants

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Selection of Pineapple Cultivars Resistant to Fusariose

Cited by 5 publications

References 0 publications

Advances in pineapple plant propagation

Advances in pineapple plant propagation

Improving pineapple micropropagation protocol through explant size and medium composition manipulation

New Pineapple Somaclonal Variants: P3R5 and Dwarf

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