Selective collection and detection of leukemia cells on a magnet-quartz crystal microbalance system using aptamer-conjugated magnetic beads

A New Concept for Efficient Sensitivity Amplification of a QCM Based Immunosensor for TNF-α by Using Modified Magnetic Particles under Applied Magnetic Field

“…24,25 In the present study it was found that the magnitude of frequency shift due to the additional magnetic system was directly dependent on both the strength of magnetic field and the concentration of the target antigen, TNF-α, which was immunologically bound to the immobilized antibody. The detection limit of the system was found to be 25 ngmL −1 and the sensitivity of this QCM based biosensor system with MPs was improved 4.3 times compared with the results without the magnetic system.…”

“…Most combined studies of QCM coupled with magnetic beads were for selective collections or separations of biological materials such as proteins or cells. 24,25 A schematic diagram of the sensing principle of QCM measurement for TNF-α under the magnetic field is illustrated in Figure 2. As can be seen, significant signal amplification could be take place when the additional built-in residual stress incorporated with magnetic beads applied to the quartz crystal, compared with the results from conventional QCM based biosensors which uses only an antigen-antibody binding.…”

Ultrathin-film oscillator biosensors excited by ultrafast light pulses

“…Quartz crystal microbalance (QCM) detects adsorbed mass on the quartz oscillator, and it is widely used as a label-free biosensor (Muramatsu et al, 1987;Liu et al, 2003) for studying interactions among various biomolecules (Emir et al, 2009;Pan et al, 2010;Wan et al, 2010;Kunze et al, 2010). Its mass sensitivity, defined as the detectable amount of adsorbed mass per unit area per unit frequency change, is significantly improved by thinning the quartz crystal, corresponding to the increase in the fundamental resonance frequency.…”

Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer

“…Antibodies, functional peptides, such as arginine-glycine-aspartic acid-serine (RGDS) tetrapeptide, have been used to functionalize electrode for capturing cells via the specific binding between these molecules and cellular surface units [10][11][12][13]. Moreover, aptamer-based interface has also been used for enrichment and detection of multiple cancer cells [14]. Despite the extensive effort launched, a growing interest has been currently focused on the use of lectin-carbohydrate recognition specificities as an alternative way for designing biominic interface to anchor cells because lectin-carbohydrate interactions play a crucial role in many cellular processes including cell adhesion, trafficking, metastasis, and immune response [15][16][17].…”