Selective Determination of Adenine-Containing Compounds by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Tseng, Hua-Ming; Dadoo, Rajeev; Zare, Richard N.

doi:10.1006/abio.1994.1453

Cited by 54 publications

(26 citation statements)

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“…Several methods have been reported to measure adenine nucleotides (ATP, ADP, and AMP) in biological samples, including capillary electrophoresis (CE) [9][10][11], 31 P nuclear magnetic resonance spectroscopy (NMR) [12,13], gas chromatography (GC) [14,15], ion-pair reversed-phase HPLC [16][17][18], anion-exchange chromatography [19], and nanotechnology [20,21]. Only Tseng et al used capillary electrophoresis with laser-induced fluorescence detection to accomplish the simultaneous analysis of ATP, ADP, AMP, and cAMP [22].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

Zhu¹,

Wang²,

Wang³

et al. 2016

Acta Chromatographica

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Summary. In order to assess the contribution of adenosine triphosphate and its metabolites to the cellular metabolism process in Saccharomyces cerevisiae, it is very important to simultaneously determine the relative concentrations of ATP and its metabolites. In this study, a fast, simple reversed-phase high-performance liquid chromatography with high selectivity was developed to simultaneously measure adenosine triphosphate and its metabolites (adenosine diphosphate, adenosine monophosphate, and cyclic adenosine monophosphate) in yeast. The method was performed under the gradient grogram, and the detection was monitored at 254 nm. Analysis was achieved within 25 min. The four components can be detected with linear response over the concentration range from 1 to 100 mg L −1 with excellent correlation coefficients (r 2 ) > 0.999. The recovery of the four analytes was 92.9%, 90.4%, 99.1%, and 105.1%, respectively. To demonstrate the good analysis of yeast samples, changes in the four adenine nucleotides levels caused by caloric restriction in yeast were determined. It is expected that the current method may contribute to further metabolomics and system biology investigations of yeast.

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Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

Zhu¹,

Wang²,

Wang³

et al. 2016

Acta Chromatographica

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“…It is important to separate the analytes from the interfering background for accurate quantitation. Capillary electrophoresis (CE), whose separation is based on the different mobility of charged analytes in the presence of an external electric field, is often a suitable approach for separating the highly charged compounds such as nucleotides [16][17][18]. A number of volatile buffers such as ammonium acetate and acetic acid have been shown to provide adequate resolution for nucleotide separation [19][20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

Cai

Fung²,

Sinhababu

2003

Electrophoresis

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A capillary electrophoresis-ion trap mass spectrometry method with a time-segment program was developed to simultaneously analyze Ziagen and its phosphorylated metabolites such as carbovir monophosphate, carbovir diphosphate, and carbovir triphosphate. By using the time-segment program, the positively charged nucleoside analog and negatively charged nucleotides were separated and detected in a single electrophoretic run. The limits of detection were less than 2 micro M for all of the analytes. Calibration curves of the compounds showed excellent linearity over the range of 2-100 micro M. The capability of the method was demonstrated by analyzing Ziagen and its phosphorylated metabolites that were spiked in cellular extracts of human peripheral blood mononuclear cells at 20 micro M levels. Some endogenous nucleotides such as adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate, were also detected in the cellular extracts.

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“…Hence, the analysis of these bases has great significance to the bioscience and clinical diagnosis [26]. Many methods, such as the spectroscopic methods coupled with chromatography or electrophoresis [27,28] and electrophoresis with electrochemical detection [29,30], have been developed for the detection and quantification of purine bases in nucleic acids. Compared to other methods, the electrochemical technique is attractive owing to its high sensitivity, inherent simplicity, miniaturization and low cost.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Application of a Novel Modified Electrode Based on Multiwalled Nanotubes/Cerium(III) 12‐Tungstophosphoric Acid Nanocomposite

et al. 2008

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A novel multiwalled nanotubes (MWNTs)/Cerium(III) 12-tungstophosphoric acid (CePW) nanocomposite film glassy carbon electrode was prepared in this paper. Electrochemical behaviors of the CePW/MWNTs modified electrode were investigated by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). This modified electrode brought new capabilities for electrochemical devices by combining the advantages of carbon nanotubes, rare-earth, and heteropoly-acids. The results demonstrated that the CePW/MWNTs modified electrode exhibited enhanced electrocatalytic behavior and good stability for the detection of guanine and adenine in 0.1 M PBS (pH 7.0). The experimental parameters were optimized and a direct electrochemical method for the simultaneous determination of guanine and adenine was proposed. The detection limit (S/N ¼ 3) for guanine and adenine was 2.0 Â 10 À8 M and 3.0 Â 10 À8 M, respectively. Further, the acid-denatured calf thymus DNA was also detected and the result was satisfied.

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Selective Determination of Adenine-Containing Compounds by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Cited by 54 publications

References 0 publications

Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

Capillary electrophoresis‐ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

Fabrication and Application of a Novel Modified Electrode Based on Multiwalled Nanotubes/Cerium(III) 12‐Tungstophosphoric Acid Nanocomposite

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