“…Indeed, it has been demonstrated both theoretically and experimentally that a low ambient pressure is beneficial to the infiltration process since it minimizes the effects of gas trapping in the LCs and contributes to drastically reduce the air volume that remains eventually encapsulated at the hole bottom (Leonard et al, 2000;Schuller et al, 2003;van der Heijden et al, 2006a-b). The residual air content is finally degassed through the LC column (Nielsen et al, 2005;van der Heijden et al, 2006a-b). Infiltration times of several minutes up to few hours were adopted in order to guarantee a complete gas evacuation and thus a perfect hole infiltration.…”