Selective purification of protease from ginger and sodom apple by ultrasound assisted liquid-liquid microextraction using natural deep eutectic solvent

Balaraman, Harish Babu; Rathnasamy, Senthilkumar

doi:10.1016/j.microc.2019.104132

Cited by 31 publications

(20 citation statements)

References 46 publications

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“…Some compounds were eluted in longer times (26 and 45 minutes) and the ultrasound method allowed to observe these peaks with higher intensity, which indicates the possible extraction of components with greater hydrophobicity due to the longer retention times. Balaraman and Rathnasamy (2019), when evaluating the factors that most influenced the extraction yield of proteases obtained by ultrasound, found that the analysis time and temperature stood out. Many factors can alter the protein extraction process, that is why it is important to optimize the extraction conditions in order to improve the characteristics of the desired product.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

et al. 2021

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The search for origin plant-based proteases increases gradually due to their diversity and stability over a wide range of pH and temperature. Artichoke (Cynara scolymus) flowers are a proteolytic vegetable source already studied, but their leaves are scarce in this respect. Thus, the objective of this research was to obtain extracts of artichoke leaves with different buffers and extraction methods as an alternative proteolytic source and plant coagulant, as well as the separation and comparison of the protein profile of these extracts. The methodology used was based on extraction with sodium citrate buffer (pH 3), sodium acetate (pH 5) and Tris-HCl (pH 7) by mechanical stirrer (MS) and ultrasound (US); protein determination; proteolytic activity (PA) and specific activity (SA); milk clotting activity (MCA) and rennet substitution potential (RSP); high- performance liquid chromatography analysis (HPLC) with UV-Vis detector and principal component analysis (PCA). Extracts of Cynara scolymus leaves showed high results with Citrate-US for the parameters PA (14.38), SA (19.71), MCA (440) and RSP (30.60) compared to other treatments. The extracts with citrate and acetate presented a quick coagulation time (max 3 min). The HPLC analysis enabled the separation of the different protein compounds present in the extracts and most expressive peaks in the samples with Citrate-MS and Acetate-MS; and isolated peaks for Citrate-US. It was concluded that extracts of artichoke leaves with citrate and acetate buffer attributed satisfactory results to act as plant coagulant, as well as to carry out further studies for the purification of proteolytic enzymes and application in cheeses.

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Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

et al. 2021

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“…However, its application becomes trivial due to various disadvantages like the high cost of synthesis, toxic in nature and variable recovery. Deep eutectic solvents (DES) are the modern age solvents used for the extraction of commercially important therapeutic enzymes 16,17 , occurs as the homogenous mixture of hydrogen bond acceptors (HBA) and hydrogen bond donors (HBD) 18,19 . Various types of DES have been established based on the choice of HBA and HBD of which ampiphilic 20 , protic 21 and natural 16 deep eutectic solvents are commonly known types.…”

Section: One-pot Simultaneous Production and Sustainable Purificationmentioning

confidence: 99%

“…Deep eutectic solvents (DES) are the modern age solvents used for the extraction of commercially important therapeutic enzymes 16,17 , occurs as the homogenous mixture of hydrogen bond acceptors (HBA) and hydrogen bond donors (HBD) 18,19 . Various types of DES have been established based on the choice of HBA and HBD of which ampiphilic 20 , protic 21 and natural 16 deep eutectic solvents are commonly known types. Natural derivatives like carbohydrates 22 , naturally occurring secondary metabolites like betaine 23 , glycerol 24 and biologically obtained acids like citric acid 25 are used as HBA and HBD to form natural deep eutectic solvents (NADES) 17 .…”

Section: One-pot Simultaneous Production and Sustainable Purificationmentioning

confidence: 99%

“…NADES has been studied for the processing of biomass and improved saccharification low toxicity, easy recycling and biodegradability 26 . Extraction of various commercial and therapeutic compounds by ABS with NADES as primary solvent andNa 2 SO 4 for forming immiscible two-phase have been established for protease 16 and immunoglobulins 17 . NADES have been proven to be biocompatible extraction medium maintaining the activity, stability and structure of laccase 23 .…”

Section: One-pot Simultaneous Production and Sustainable Purificationmentioning

confidence: 99%

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One-pot simultaneous production and sustainable purification of fibrinolytic protease from Bacillus cereus using natural deep eutectic solvents

Rathnasamy

Durai

Vigneshkumar

et al. 2020

Sci Rep

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“…Beyond encasing several advantages of being super cial and low cost in synthesis, they are task-speci c and are facile for regeneration 24 . Earlier investigations have employed DES as a task-speci c medium to purify therapeutically important enzymes like protease 25 and immunoglobulins 26 . Therefore, these investigations have concluded the selective and task speci c nature of NADES making it a suitable medium for extractive fermentation of naringinase 27,28 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Production and Sustainable Eutectic Mixture Based Purification of Narringinase With Bacillus Amyloliquefaciens by Valorization of Tofu Wastewater

Balaraman

Purushotaman

Chandramouliswaran

et al. 2022

Preprint

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Sustainable one-pot production and purification of naringinase using natural deep eutectic solvent based extractive fermentation is being executed in the current investigation. Tofu wastewater was used as alternative low-cost media for sustainable production of naringinase. Five natural deep eutectic solvents were prepared and their physicochemical properties were determined as a function of temperature. Optimization of essential variables in extractive fermentation for determining effective concentration of NADES at (74.5% w/w), Na2SO4 (15% w/v) and tofu wastewater (1.5% w/w) to achieve optimal naringinase yield of (249.6 U/ml). Scale up of naringinase production with a 3 L custom made desktop bioreactor was accomplished and effective regeneration of NADES was established. Quantification of the resulting active enzyme was done by size exclusion chromatography (736.85 U/mg). Ultrapure enzyme fraction was obtained with anion exchange chromatography yielding maximum purity of (63.2) and specific enzyme activity of (3516 U/mg). The in-vitro debittering activity of resulting ultrapure naringinase was determined with grape juice.

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Selective purification of protease from ginger and sodom apple by ultrasound assisted liquid-liquid microextraction using natural deep eutectic solvent

Cited by 31 publications

References 46 publications

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

Artichoke leaf extracts: Proteolytic activity, coagulant and HPLC analysis

One-pot simultaneous production and sustainable purification of fibrinolytic protease from Bacillus cereus using natural deep eutectic solvents

Simultaneous Production and Sustainable Eutectic Mixture Based Purification of Narringinase With Bacillus Amyloliquefaciens by Valorization of Tofu Wastewater

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